药物研究
HUANG Yanming;LIU Hengming;LUO Yan
2005, 24(2): 106-0108.
To investigate the absorption characteristics and toxicity of hematoporphyrin monomethyl ether （HMME） in cultured pterygium fibroblasts so as to provide experimental basis for the photokinetic treatment of pterygium.Methods Cultured fibroblasts from incipient and recurrent pterygium were inoculated into 24 well plates and separately incubated for 0,5,10,30,45,60,120 and 180 min with serum free media containing HMME in concentrations of 0,20,40,80,100,120,140 and 160μg·mL1 ,respectively. The content of intracellular photosensitizer was determined with fluorescence analysis. The concentrationcontent and timecontent relation curves of the absorption of the photosensitizer by the pterygium fibroblasts were drawn .The cells were then inoculated into 96 well plates and separately incubated for 0,5,10,20,30,45,60,120 min with media containing HMME in concentrations of 0,2.5,5,10,20,40,80,160 μg·mL1 ,respectively. The absorbance (A) of each well at a wavelength of 550 nm was determined with MTT colorimetry .The inhibition rate of the proliferation of every set of cells was calculated. ResultsThe amount of HMME absorbed by fibroblasts from incipient and recurrent pterygium was increased along with the increase in the concentration of the photosensitizer and prolongation of the incubation time .The absorption of HMME by the cells was relatively fast 10 min after the addition of the photosensitizer, reaching soon afterwards a plateau phase. 45 min later the absorption rate was once more increased, but gradually. Fibroblasts from incipient and recurrent pterygium were shown to be inhibited after 1 h of incubation in media containing HMME at concentrations higher than 20 and 40 μg.mL1,respectively .Conclusion HMME was shown to be readily absorbed by fibroblasts of pterygium . The amount of HMME absorbed by the cells correlated with the concentration of the photosensitizer and the time of incubation.
