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药物研究

Changes in and Regulation of PKC Activity in Murine Peritoneal Macrophages Following Severe Scald

WANG Yong;PENG Daizhi;HUANG Wenhua;ZHOU Xin

2003, 22(6): 367-369.

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To study the changes in the activity of protein kinase C(PKC)in murine peritoneal macrophages (PMФs) at different time intervals after severe scalding and to assay the activity of the nuclear factor κB (NFκB) of PMФs under the action of H7, a specific PKC inhibitor. These experiments were designed in an attempt to clarify the mechanisms underlying the functional disorders of macrophages from the angle of signal transduction.Methods:The experimental model was set up with mice which had been subjected to fullthickness grade Ⅲ scalding of 15% of the TBSA(total body surface area). From these animals, PMФs were collected. Both membrane and cytoplasmic PKC activities were assayed with the isotope(γ32PATP) incorporation method, while the NFκB activity was detected with the electrophoretic mobility shift assay (EMSA). Results:Both membrane and cytoplasmic PKC activities were upregulated after scalding and the former showed a positive correlation with the changes in TNFα mRNA. The NFκB activity decreased under the action of H7. Conclusions: Activity of both PKC and NFκB was shown to be markedly increased following severe scalding. The PKCNFκB signal pathway seemed to be involved in the regulation of TNFα expression in the macrophages.

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药物研究

Effect of Thyroxin on the Gene Expression of Uncoupling Protein2 in White Adipose Tissue of the Rat

YI Yingchun;LIN Hanhua

2003, 22(6): 369-371.

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To investigate the effect of thyroxin on the gene expression of uncoupling protein2(UCP2) in white adipose tissue(WAT) of the rat.Methods：30 normal male Wistar rats were randomly divided into three groups: control group, hyperthyroid group and hypothyroid group, with 10 rats per group. The hyperthyroid or hypothyroid status of the rats was made by administration of Lthyroxine or methimazole. The serum total T3, T4 concentrations were measured by radioimmunoassay(RIA). The mRNA levels in UCP2 in WAT were detected by semiquantitative reverse transcription polymerase chain reaction(RTPCR). Results：Compared with the normal control group, a 1.4 fold increase and a 1.2 fold decrease in UCP2 mRNA were detected in hyperthyroid and hypothyroid groups respectively. The differences were both highly significant.Conclusion：Gene expression of UCP2 in rat WAT was upregulated by thyroxin.

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药物研究

A Study of Methods for Extraction of Lentinan and Amino Acids From the Foot Body of Lentinus Edodes

MA Changqing;XIA Rong;PENG Yan;HE Xiuping

2003, 22(6): 372-374.

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To study the methods for extraction of lentinan and amino acids from the foot body of lentinus edodes. Methods:Lentinan and amino acids were extracted from the foot body and cap of lentinus edodes with cellulase and warm water separately. The contents of these active principles were determined by spectrophotometry.Results:The contents of lentinan in extracts of the foot body and cap of the mushroom that had been subjected to enzymatic hydrolysis were increased by 54% and 44%, respectively, as compared with those in extracts of the plant without enzymatic hydrolysis. However, with the same enzymatic hydrolysis method, the contents of amino acids in extracts of the food body and cap of lentinus edodes were increased only by 10% and 15%, respectively. Conclusion:It seems advisable to use the cellulase hydrolysis method for the extraction of lentinan and warm water hydrolysis method for that of amino acids from the foot body of lentinus edodes, respectively.

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药物研究

A Study of the Conditions for Enzymehydrolysis of the Conjugates of Diazepam Metabolites in the Urine

GUO Shengcai;ZHANG Yurong;GUO Youmei;ZOU Xiaohua;ZHENG Lixing;WANG Rong

2003, 22(6): 374-375.

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To study the conditions for enzymehydrolysis of the conjugates of diazepam metabolites catalyzed by βglucuroridase in the unine. Methods:An orthogonal design on the basis of table L9(34)was used in the experiment. An internal standard and appropriate amount of βglucuronidase were added to 2 mL of urine sample, which was then incubated at different temperatures and in different time periods for hydrolysis. The products were extracted with 5 mL of nhexane: dichloromethane(5∶3) and the extract was dried by volatilization. The residue was dissolved with 50 μL of methanol, and 10 μL of which were added onto the HPLC system.Results:It was shown by analyses of variance of the ratios of peak areas of hydrolyzates to those of internal standards that the optimal conditions for enzymehydrolysis of urine glucuronide conjugates of nordiazepan, temazepam and oxazepam were 55℃, 5 h, 5 000 units of βglucuronidase, 55℃, 2 h, 1,000 U of the enzyme, and 55℃, 2 h, 5,000 U of the enzyme, respectively. Conclusion:The optimal condition for enzymehydrolysis of the conjugates of diazepam metabolites in 2 mL of urine was shown to be 55℃, 5 h, and 5,000 units of βglucuronidase.

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皮肤病性病用药专栏

Cuoyanling No.1 in the Treatment of 103 Patients with Acne Vulgaris

LI Shenqiu

2003, 22(6): 378-379.

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To evaluate the therapeutic effectiveness and safety rate of cuoyanling No.1 in the treatment of acne vulgaris.Methods:103 patients with acne vulgaris served as the subjects of the study. The affected areas were painted with cuoyanling No.1 prepared by ourselves once every morning and evening after facewash. The course of treatment lasted 4 weeks. Changes in lesions including comedones, inflammatory papules, pustules and nodules as well as adverse reactions were kept under careful observation.Results:The clinical response was excellent in 28 patients(27.2%), good in 61(59.2%) and favorable in 11(10.7%). The overall effective rate was 86.4%.Conclusion:Cuoyanling No.1 was shown to be safe and effective in the treatment of acne vulgaris

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药物与临床

Treatment of 57 Cases of Hypertrophic Scars and Keloids with Contractubex Gel

LI Wengang;XU Chengrong;CHEN Xingping;CHEN Yingling

2003, 22(6): 380-382.

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To test the clinical effectiveness of contractubex gel in the treatment of small area hypertrophic scars and keloids. Methods:Hypertrophic scars and keloids in 57 patients were painted with contractubex gel four times a day in a period of 6 consecutive months. After each painting, the local areas were rubbed gently for 5－10 min to promote absorption of the drug. Changes in the size, hardness, and color of the scars as well as the patients’ symptoms including itch and pain were scored at the termination of the course of treatment. Results:The overall effective rate of contractubex gel was 61.4% in all of the patients studied. In patients with a duration of illness within a year, the effective rate was 76.7%, while that in those with a course longer than a year was 44.4%. Conclusion:Contractubex gel was shown to be a new and effective drug for the treatment of hypertrophic scars and keloids, in particular for those patients with a relatively short course of disease.

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皮肤病性病用药专栏

Treatment of 117 Cases of Herpetic Dermatosis with Rhubarb Injection

SHENG Wanxiang;FENG Yi;SONG Jiquan;LIAO Fucui;SONG Qin

2003, 22(6): 382-383.

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To survey the clinical therapeutic effectiveness of rhubarb injection in the treatment of herpetic dermatosis.Methods:157 patients with herpetic dermatosis including 59 cases of herpes zoster and 98 of herpes simplex served as the subjects of the study and were randomly divided into 2 groups. 117 patients of the treatment group were given each 2 mL of rhubarb injection I.M., q.d. or b.i.d., supplemented locally with wet dressings with rhubard injection over the skin lesions. 40 patients of the control group were given each 0.1g of acyclovir PO, q.i.d., while skin lesions were treated with dressings soaked with 0.1% ethacridine or local smearing of acyclovir ointment. The course of treatment in both groups lasted 5 days. A second course was ordered if necessary.Results:The effective rates of rhubarb injection in the treatment of herpes zoster and herpes simplex were 82.1%and 98.7%, respectively, while those for herpes zoster and herpes simplex in patients of the control group were 85.0% and 100.0%, respectively. The differences between effective rates of the two groups were not significant (P＞0.05). However, the effects with respect to pain relief as well as crust formation and repair of skin lesions were far much better in patients of the treatment group than in those of the control group(P＜0.05). Conclusion:Rhubarb injection was shown to have good clinical therapeutic effects in the treatment of herpetic dermatosis. The course of treatment was relatively short, patients’ symptoms faded rapidly and no toxic and side effects were encountered.

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皮肤病性病用药专栏

Treatment of 71 Cases of Nongonococcal Prostatitis with Azithromycin

LIU Zhenfu;LI Wen;LIU Zehu

2003, 22(6): 384-385.

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Objective:To evaluate the therapeutic effectiveness of azithromycin in the treatment of nongonococcal prostatitis caused by chlamydia trachomatis (Ct) and ureaplasma urealyticum (Uu).Methods:71 patients with nongonococcal prostatitis caused by Ct and Uu were given each 1 g of azithromycin PO as an initial dose followed by 0.5 g PO q.d. for 14 consecutive days. Results:The overall clinical effective rate was 94.37%. Pathogens were eliminated in 73.24% of the patients. There were no major adverse reactions. Conclusion:Azithromycin was shown to have a fairly good therapeutic effect in the treatment of nongonococcal prostatitis, with rapid relief of clinical symptoms and rare adverse reactions.

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皮肤病性病用药专栏

A Clinical Study of the Effect of Shengfaling Tincture in the Treatment of 280 Cases of Alopecia Areata

MA Pingbo

2003, 22(6): 385-386.

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Objective:To survey the therapeutic effectiveness of shengfaling tincture in the treatment of alopecia areata induced by disorder of autoimmune function. Methods:400 cases of alopecia areata were randomly divided into two groups, with 280 patients in the treatment group and 120 in the control group. The study was carried out with a single blind method. The affected areas in patients of the treatment group were painted t.i.d. with shengfaling tiucture followed by gentle rubbing until a slight local reddening appeared. Skin lesions in patients of the control group were painted t.i.d. with 2% minoxidil lotion. The course of treatment in both groups lasted 7 days. The overall therapeutic effectiveness, serum level of TNFα and autoantibodies were kept under observation. Results:The overall effective rate in patients of the treatment group was 93%, with 171 cases basically cured, 92 improved and only 17 unaffected. In contrast, the overall effective rate in patients of the control group was 75.8%, with 58 cases basically cured, 33 improved and 29 unaffected. The differences between the 2 groups were significant (P＜0.05). The fall of serum TNFα and autoantibody levels in patients of the treatment group was far more striking than that of the control group(P＜0.01). Conclusion:Shengfaling tincture was shown to be effective in the treatment of alopecia areata induced by disorder of autoimmune function.

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药物与临床

The Effect of Essential Phospholipids in the Treatment of Alcoholic Hepatopathy

GAN Xueting;BAI Xianguang;LUO Hong

2003, 22(6): 392-393.

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Objective:To study the therapeutic effectiveness and safety rate of essential phospholipids (EPL) in the treatment of alcoholic hepatopathy. Methods:45 patients with alcoholic hepatopathy were given each 500 mg of EPL injection in 250 mL of 5% or 10% glucose solution administered by slow IV instillation q.d., supplemented with routine supportive measures. The course of treatment lasted 8 weeks. All patients were subjected to routine physical examination, assay of liver function and liver fibrosis indices including hyaluronic acid and PⅢP before and after the termination of treatment. Results:At the termination of the treatment, the rate of excellent clinical response was 57.8%(26/45), that of moderate improvement 28.9%(13/45) and only 6 patients (13.3%) were unaffected. Conclusion:EPL was shown to be a safe and effective drug in the treatment of alcoholic hepatopathy.

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药物与临床

Treatment of 102 Cases of Functional Dyspepsia with Mosapride

CHEN Ken;LIANG Jian;MAI Haiyan;WANG Wanmei;ZHU Bin

2003, 22(6): 394-396.

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Objective:To observe the therapeutic effectiveness and safety rate of mosapride in the treatment of functional dyspepsia (FD). Methods:168 patients with FD were randomly divided into 2 groups. 102 patients of the treatment group were given each 5 mg of mosapride, PO, t.i.d.. 66 patients of the control group were given each 10 mg of domperidone, PO, t.i.d.. The course of treatment in both groups lasted 4 weeks. Results:The total effective rate in patients of the treatment group was 89.2%, which was significantly higher than that(58.1%) of the control group(P＜0.01). 2 weeks after the beginning of treatment, the chief clinical symptoms in patients of the treatment were found to remit strikingly. After 4 weeks of medication, remission of symptoms in patients of the treatment group was obviously better than that at the second week of treatment (P＜0.01). Remission of all clinical symptoms except vomiting was more prominent in patients of the treatment group than that of the control group (P＜0.05 or 0.01).Conclusion:Mosapride was shown to be a safe and effective drug in the treatment of FD.

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药物与临床

Pinaverium Bromide in Combination with Bifidobacteria in the Treatment of Irritable Bowel Syndrome

SHEN Minning;CHEN Zhitan

2003, 22(6): 396-397.

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Objective:To compare the curative effect of pinaverium bromide in combination with Bifid. Triple viable power with that of pinaverium bromide alone in the treatment of irritable bowel syndrome(IBS). Methods:84 patients with IBS were randomly divided into two groups. 44 patients of the treatment group were given each 50 mg of pinaverium bromide, PO, t.i.d. and two pills of Bifid. Triple viable power, PO, t.i.d.. 40 patients of the control group were given 50 mg of pinaverium bromide alone, PO, t.i.d.. The course of treatment in both groups lasted 4 weeks. Results:The curative effect in patients of the treatment groups was 97.7%, while that of the control group was only 80.0%(P＜0.05). The effects of remitting abdominal pain, abdominal distension, diarrhea, constipation and mucous stool in patients of the treatment group were far much better than those of the control group.Conclusion:Pinaverium bromide in combination with bifid triple viable power was shown to be effective in the treatment of IBS.

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药物与临床

Supplementation of Ofloxacin or Amikacin to the Therapeutic Plan for the Retreatment of Pulmonasy Tuberculosis: An Analysis of the Curative Effects

GU Hongsheng;FENG Qian;SHEN Jian

2003, 22(6): 398-399.

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Objective:To study the method for retreatment of patients with multipledrug resistant pulmonary tuberculosis (MDRTB), taking account of the curative effect, prognosis and prospect of patients’ condition. Methods:49 patients with refractory pulmonary tuberculosis served as subjects of the study. Before admission to our hospital, the sputa of these patients had been positive for tubercle bacilli over a long period of time and they had been subjected to more than two courses of treatment with the 25 HRZ/4HR plan without curative effect. According to the history of medication and the results of drug sensitive tests, 29 patients resistant to HS or RS were submitted to the OFL (ofloxacin) plan: 3HZOE/6HOE, while 20 patients resistant to HRS, to the OFL+AK(amikacin) plan:2HZOAE/7HOE.(H＝Isoniazid, Z＝pyrazinamide, E＝Ethambutol, A＝Amiracin, O＝Ofloxacin). The period of followup survey lasted 12 months. Results:The time period for sputa to turn negative for tubercle bacilli in patients submitted to the OFL plan was longer than that in patients submitted to the OFL+AK plan. At the termination of the course of treatment, the rate of sputa turning negative for tubercle bacilli was somewhat lower in patients submitted to the OFL plan than that in patients submitted to the OFL+AK plan. The recurrence rate in patients submitted to the OFL plan was 9.0% while that of patients submitted to the OFL＋AK plan, 0%. Conclusion:The OFL＋AK plan was shown to be better than the OFL plan in the retreatment of MDRTB.

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药物与临床

Treatment of 43 Cases of Depression with Qingnaofushenye

TANG Huiming

2003, 22(6): 400-401.

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Objective:To evaluate the therapeutic effectiveness of qingnaoyefushen in the treatment of depression.Methods:86 patients with depression were randomly divided into two equal groups. Patients of the treatment group were given each 40 mL of qingnaofushenye PO, b.i.d.. Patients of the control group were treated with amitriptyline (AT), they were given each on the first day 25mg of AT PO, b.i.d.. The dose of AT was increased by 25mg PO b.i.d every other day until a dosage of 100 mg PO, b.i.d. was reached. The Zung depression scale was used to assess the therapeutic effectiveness before the treatment as well as two, four, and eight weeks after the beginning of the medication.Results:The effective rates in patients of the treatment group 2,4 and 8 weeks after the beginning of treatment were 83.7%, 86.0%, and 88.4%, respectively, while those in patients of the control group were 86.0%, 88.4% and 88.4%, respectively. The differences between the two groups were insignificant(P＞0.05). Conclusions:As a traditional Chinese medicine, qingnaofushenye may be used as an antidepression drug in the clinic.

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药物制剂

Optimization of the Extraction Technology for Water Soluble Components in Wusan Granules with an Orthogonal Test

XIN Zhiwei;ZANG Zhihe;ZHU Weidong;YOU Hongtao;CHEN Haihong;SHI Jinping;GAO Liyuan

2003, 22(6): 412-413.

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Objective:To optimize the extraction technology for water soluble components in wusan granules.Methods:An orthogonal test with the use of an L9(3)4 orthogonal table was adopted for the optimization. Recovery rate of the dry extract and the content of emodin served as the indices. The 4 factors influencing the decoction extraction of water soluble components of the drug——time of soaking of the drug, amount of water added, time of decoction and times of decoction were kept under observation. Results:The optimized extraction technology was as follows: 10 volumes of water were added to 1 volume of the crude drug, the drug was soaked for 60 min and decocted for 3 times, each decoction lasting 60 min. Conclusion:The method was shown to be suitable for the decoction extraction of water soluble componeuts in wusan granules.

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药品质量控制

Content Determination of Baicaline in Fufangyuxingcao (Composite Houttuynia) Tablets with ReversedPhase HPLC

ZHANG Hangshan;WANG Hongxing;CUI Ping;LI Bin

2003, 22(6): 419-420.

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Objective:To determine the content of baicaline in composite houttuynia tablets with reversedphase HPLC so as to control the quality of the preparation.Methods:Baicaline was separated with a C18 chemical bonding silica gel column. A mixture of methanolwateracetic acid (40∶60∶1) served as the mobile phase. UV detection wavelength was set at 274 nm.Results:The resolution degree of baicaline peak and peaks of other components was 2.1; the number of theoretical plates calculaled for baicaline peak was 15,590; the average rate of recovery of the added sample was 98.8%(n＝5). The RSD obtained from 5 independent determinations was 0.92%. There was a good linear relationship between the quantity of baicaline sample and the absorption area value within a range of 0.25～2.5 μg of baicaline. Conclusion:The method was shown to be highly accurate and reproducible in the content determination of baicaline in fufangyuxingcao tablets.

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