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  • 01 April 2015 Volume 34 Issue 4
      
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  • Study of Inhibition Fibrosis of MicroRNA-25 on Diabetic Nephropathy by Regulating Expression of MAP2K4
    WANG Xiaoli, LIU Jieting, ZHANG Chunlei, WANG Chaonan, FENG Biao, CHU Yanhui, ZHANG Tao
    2015, 34(4): 425-431. https://doi.org/10.3870/yydb.2015.04.001
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    Objective To determine the expression level of microRNA-25 in animal models of diabetic nephropathy and human renal tubular epithelial cells (HK-2) cultured in different conditions, and to explore its regulating effect on the fibrosis in diabetic nephropathy. Methods The expression of microRNA-25 was detected by real-time PCR.The downstream target protein of microRNA-25 was verified by bioinformatic prediction, transient transfection of cells and Western blotting. Results MicroRNA-25 was down-regulated in animal models of diabetic nephropathy and HK-2 cells which were cultured in high-glucose medium (P＜0.01).MAP2K4 might be the downstream target protein of microRNA-25.Overexpression of microRNA-25 reduced the protein expression of MAP2K4 and α-SMA (P＜0.01). Conclusion MicroRNA-25 inhibits the fibrosis in diabetic nephropathy by regulating the expression of MAP2K4.
  • Study of Clotrimazole on Cell Apoptosis in Rat Liver After Ischemia-reperfusion Injury
    XU Jing,CHEN Jie,LUO Ziling,GUAN Biqiong,HE Binhong,SUN Pingping,YUAN Fang
    2015, 34(4): 432-434. https://doi.org/10.3870/yydb.2015.04.002
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    Objective To investigate the effect of clotrimazole on apoptosis of hepatic cells after ischemia-reperfusion injury and its mechanism. Methods Hepatic ischemia-reperfusion rat model was established.Thirty-two male Sprague-Dawley rats were randomly allocated into sham-operated group,model control group,low dose clotrimazole group and high dose clotrimazole group.Apoptosis in hepatic tissue was assessed by TUNEL method.Protein expression levels of CYP3A1,Bcl-2,Bax and PARP were measured by Western blotting. Results As compared with model control group,the apoptosis rate,tissue injury,activity of plasma enzymes and the Bax/Bcl-2 expression ratio were reduced in low and high dose clotrimazole groups.The apoptotic index in both clotrimazole-treated groups was lower than that of model control group with statistically significant difference.CYP3A1 expression was significantly induced by clotrimazole compared to the sham-operated group. Conclusion Clotrimazole may inhibit apoptosis of hepatic cells by up-regulating Bcl-2 and down-regulating Bax,thus produce a protective effect on hepatic ischemia-reperfusion injury and it is also related to the inhibition of PARP shear.
  • Effect of Propofol on Expression of Phosphorylated Tau Protein and Beta-amyloid (1-42) Protein in Hippocampus of Offspring Rat
    SUN Qing, ZHANG Liang, CHEN Baicheng, CHEN Xian, ZHANG Guoqin
    2015, 34(4): 435-439. https://doi.org/10.3870/yydb.2015.04.003
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    Objective To study the effects of propofol exposure during pregnancy on space cognitive and exploration abilities and expression of phosphorylated tau protein (P-tau) and beta-amyloid protein[Aβ(1-42)] in hippocampus of the offspring. Methods Sprague-Dawley female (n＝24) and male rats (n＝8) of three months old were mated at the same cage at the ratio of 3:1.Pregnant rats were randomly divided into early group (group E), medium group (group M), late group (group L) and control group (group C), with 6 rats in each group.Groups E, M and L received propofol 80 mg·kg－1·d－1 for 7 consecutive days.Propofol was replaced with equal volume of physiological saline in group C.Learning and memory of the 30-day offspring rats were assessed by using Morris water maze test.Then offspring rats were sacrificed to determine the expression of P-tau and Aβ(1-42) in the hippocampus by ELISA and immunohistochemistry. Results The learning and memory abilities were declined significantly in group E (51.20±8.11) s, group M (36.00±6.44) s and group L (47.20±12.30) s, as compared with group C (65.60±7.23) s (all P＜0.05).The result of immunohistochemistry and ELISA showed that expression of Aβ(1-42) and P-tau in hippocampus was significantly higher in group M than in groups E, L and C[(immunohistochemistry: Aβ(1-42), (27.38±5.90) vs.(12.65±2.08), (13.79±3.37), and (65.60±7.23); P-tau, (26.35±5.83) vs.(13.65±3.46), (14.56±3.82), and (8.49±1.20); ELISA: Aβ(1-42), (88.6±7.43) vs. (71.60±6.79), (13.79±3.37), and (65.80±6.28); P-tau, (230.13±8.22) vs.(210.42±2.20), (210.95±1.75), and (200.65±1.57)] (all P＜0.05). Conclusion Multiple propofol injections may impair rat offspring’s space cognitive abilities and exploration abilities, and the impairment is especially obvious in second trimester of pregnancy, which may be associated with over-expression of P-tau and Aβ(1-42).
  • Effects of Nuciferine on Mice with Hyperlipidemia and Its Mechanisms
    ZHANG Lijing,AI Yaowei,WANG Zhengqiang
    2015, 34(4): 440-443. https://doi.org/10.3870/yydb.2015.04.004
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    Objective To investigate the effects of nuciferine on hyperlipidemia in mice and to clarify the molecular mechanism. Methods Mice were divided into three groups according to the diet: normal control group (n＝10), model control group (n＝10), and the intervention group (n＝10).The normal control group was treated with common diet (ANI-76A feed: 12.4% fat, 68.8% carbohydrate, 18.8% protein).The model control group was induced with high fat diet (37.1% fat, 42.4% carbohydrate, 20.5% protein).The intervention group was supplemented with 0.5% nuciferine based on high fat diet.The mice were allowed free access to food and water for a total of 10 weeks.Several indices were analyzed in the 3 groups, including the body weight, serum lipid, lipid metabolism key enzyme, oxidative stress and metabolic pathway. Results Our results suggested that the high-fat diet-induced animal models developed obesity and dyslipidemia (P＜0.05).The body weight[(33.97±3.46) g vs.(27.62±2.87) g] and the serum lipid[(2.73±0.26) g vs.(1.91±0.21) g] were significantly decreased in the intervention group compared to the model control group (P＜0.05), except for the hypertriglyceridemia (P＞0.05).The activity of hepatic lipid metabolism key enzymes[(4.15±1.26) U·mL－1 vs.(9.01±1.34) U·mL－1] and the activity of hepatic lipase and lipoprotein lipase[(8.12±3.07) U·mL－1 vs.(13.48±3.75) U·mL－1] were elevated.Oxidative stress was also affected by nuciferine (P＜0.05).Mechanism study suggested that lipid synthesis genes (like SREBP-1c, FAS, SCD-1 and PPAR gamma mRNA) were up-regulated by high fat diet (P＜0.05), and the lipid oxidation metabolism genes, PPARα and CPT-1a mRNA, were down-regulated (P＜0.05), while the intervention group treatment reversed these changes (P＜0.05). Conclusion Nuciferine can improve hyperlipidemia, which might be related to the regulation of enzyme activity, oxidative stress and the changes of lipid synthesis and oxidative metabolism.
  • Targeting Effect of Curcumin Gelatin Microspheres in Rats in vivo
    DENG Jungang, CHEN Wei, HUANG Guihong, LI Jiang, DENG Hang
    2015, 34(4): 445-447. https://doi.org/10.3870/yydb.2015.04.006
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    Objective To observe the targeting effect of curcumin gelatin microsphere in rats in vivo. Methods Injections of curcumin gelatin microsphere and curcumin were injected via tail vein, respectively.HPLC was used to determine the content of curcumin in different organs.The pharmacokinetic parameters were calculated on the basis of compartment models by using DAS 2.0 program.Targeting efficiency was used to evaluate tissue distribution of curcumin. Results Targeting efficiency of curcumin gelatin microsphere in heart, liver, spleen, lung and kidney was 0.875, 0.121, 1.182, 5.834 and 0.896, respectively. Conclusion Curcumine gelatin microspheres can improve lung-targeting efficiency, and benefit for study on lung targeting therapeutic effect.
  • Effect of Cinobufotalin Freeze-dry Powder on Heart Rate and Electrocardiograph of Anesthesia Rats
    FENG Guosheng, LUO Pan, CHEN Jiangwei, ZHU Li,GAN Lu
    2015, 34(4): 448-451. https://doi.org/10.3870/yydb.2015.04.007
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    Objective To observe the effect of cinobufotalin freeze-dry powder on heart rate (HR) and electrocardiogram (ECG) of SD rats and to provide experimental basis for monitoring its adverse effect on heart in clinical application. Methods The drug was administered into external jugular vein at constant speed throughout the whole experiment; standard-Ⅱ limb lead monitored the HR and ECG, and then the changes in HR and ECG before and after administration of cinobufotalin were compared. Results Thirty minutes after administration of cinobufotalin injection and cinobufotalin freeze-dry powder at middle dose and high dose, HR of the rats was significantly increased as compared with blank control group[(469±40) bpm, (466±29) bpm and (484±40) bpm vs. (411±17) bpm] (P＜0.05), but soon afterwards, it returned to normal.A small number of rats also developed arrhythmia.In addition, administration of cinobufotalin significantly shortened the P-R interval[blank control group: (46.90±3.90) ms, cinobufotalin injection administration group: (39.70±2.54) ms; middle dose of cnobufotalin freeze-dry powder administration group: (37.70±3.77) ms; high dose of cinobufotalin freeze-dry powder administration group: (39.30±7.12) ms] and Q-T interval[blank control group: (61.29±9.46) ms; middle dose of cinobufotalin freeze-dry powder administration group: (55.13±4.67) ms; high dose of cinobufotalin freeze-dry powder administration group: (51.75±11.53) ms] (P＜0.05), but QRS and S-T were unchanged (P＞0.05). Conclusion Cinobufotalin freeze-dry powder has some side effects on rat heart and can increase HR, even lead to arrhythmia.
  • Three-Dimensional Conformal Radiation Therapy Combined with Tegafur for 32 Cases of Locally Recurrent Rectal Cancer
    SHU Chengrong, CAO Ailing, ZHANG Yong, WANG Hanjiao, ZHOU Jian, HE Ling
    2015, 34(4): 452-454. https://doi.org/10.3870/yydb.2015.04.008
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    Objective To evaluate the safety and feasibility of three-dimensional conformal radiation therapy combined with tegafur in treating locally recurrent rectal cancer. Methods A total of 32 patients with locally recurrent rectal cancer were treated with chemoradiotherapy (CRT). Radiotherapy was delivered to a total of 45 Gy in 25 fractions followed by a boost of 18 Gy in 10 fractions using three dimensional radiotherapy planning.Tegafur was given orally[80 mg·(m2)－1·d－1] on days 1－5, 8－12, 15－19, 22－26, 29－33, 36－40, and 43－47 during radiotherapy. Results Most of the adverse effects were mild.Grade 3－4 toxic effects occurred in 12.5% of patients.Thirty-one patients completed full course of CRT, while one patient discontinued chemotherapy due to Grade 4 thrombocytopenia.There were 3 cases (9.4%) with complete response, 21 cases (65.6%) with partial response, and the overall response rate was 75.0%.Overall pain response (complete and partial pain relief) was achieved in 96.9% of patients.The 1- and 2-year overall survival rate was 71.0% and 56.5%, respectively. Conclusion 3D-CRT combined with tegafur for locally recurrent rectal cancer is feasible with high patient compliance and tolerable toxicities.
  • Effect of Huaier Granules on Invasion and Metastasis of Colorectal Cancer SW480 Cells in vitro
    JIANG Jianwu, LI Xiaolan, LENG Yan, TAO Deding, HU Junbo, GONG Jianping
    2015, 34(4): 455-458. https://doi.org/10.3870/yydb.2015.04.009
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    Objective To investigate the effects of huaier granules on invasion and metastasis of colorectal cancer SW480 cells in vitro, and explore the basic mechanism. Methods The appropriate concentration and duration of huaier granules promoting SW480 cell apoptosis were determined by SubG1 method.Wound healing assay and transwell assay were used to observe the effect of huaier granules on SW480 cell invasion and metastasis.The changes of E-cadherin, twist, snail and vimentin at protein and mRNA levels were examined by Western blotting and Real-Time PCR. Results After treatment with huaier granules at 3.0 g·L－1 for 36 h, apoptosis of SW480 cells was most significant, and wound healing assay revealed that relative mobility was (31.36±2.39)%, compared with (61.11±1.09)% in control group (P＜0.01).Number of invaded cells per field of view was (129±12) in treatment group and (354±20) in control group (P＜0.01).After treatment with huaier granules at 3.0 g·L－1 for 36 h, protein and mRNA levels of E-cadherin were increased, while those of twist, snail and vimentin were decreased. Conclusion Huaier granules can inhibit invasion and metastasis of colorectal cancer SW480 cells in vitro through effectively depressing epithelial-mesenchymal transition.
  • Antitumor Effect of Paclitaxel for Injection (Albumin Bound)
    WANG Caixia, GAO Yuqing, LI Yanling,BI Yuxing,WEI Na,ZHAO Qian,LI Chunlei[1.CSPC Zhongqi Pharmaceutical Technology
    2015, 34(4): 459-462. https://doi.org/10.3870/yydb.2015.04.010
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    Objective To compare the antitumor effect of self-developed albumin bound paclitaxel for injection (PAB) and commercial albumin-bound paclitaxel for injection (Abraxane). Methods The antineoplastic effects of PAB and Abraxane were evaluated in H22, Lewis and RM-1 allograft tumor mouse models after repeated intravenous injection (13.4, 20.0, 30.0 and 45.0 mg·kg－1 PAB and 20.0 and/or 30.0 mg·kg－1 Abraxane, respectively). Results PAB significantly inhibited H22 tumor growth at from the doses of 13.4, 20.0, 30.0, and 45.0 mg·kg－1 ,and the antitumor effect of PAB at 20.0 mg·kg－1 was not significantly different from Abraxane at 20.0 mg·kg－1.PAB dose-dependently inhibited Lewis and RM-1 tumor growth at the doses of 20.0, 30.0, and 45.0 mg·kg－1.The no observed adverse effect level of PAB and Abraxane was 20.0 mg·kg－1 in Lewis and RM-1 bearing C57 female mice.The antitumor effect and toxicity was not significantly different between PAB and Abraxane at equivalent doses. Conclusion At the same dose level, the antitumor activity and toxicity of PAB was equivalent to those of Abraxane.
  • Inhibitory Effects of Pharbitidis Semen on Rat Hapatoma Induced by N-nitrosodiethylamine
    WU Rongmin, FANG Xiaoyan, LING Yanwu, LI Biao, HUANG Binchen
    2015, 34(4): 463-466. https://doi.org/10.3870/yydb.2015.04.011
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    Objective To study the inhibitory effect of Pharbitidis Semen on rat hepatoma induced by N-nitrosodiethylamine (NDEA). Methods SD rats were divided into normal control group, model control group and Pharbitidis Semen group.In model control group and Pharbitidis Semen group, 0.01% NDEA was applied for 90 days to induce hepatoma, and rats in Pharbitidis Semen group concomitantly received feed containing 6% Pharbitidis Semen at the dosage of 40 g·kg－1·d－1.Thirty days after the hepatoma inducement and Pharbitidis Semen administration, the rats were sacrificed to observe the pathological changes in liver, number of hepatoma nodules and liver weight.The changes of liver/body weight, serum alanine aminotransferase (ALT), γ-glutamyl transferase (γ-GT), and alkaline phosphatase (ALP) were compared.One-way ANOVA (LSD Test) was employed for statistical analysis. Results In the normal control group, the number of hepatoma nodules was 0.0±0.0, the liver weight was (9.87±1.30) g, the ratio of liver/body weight was (2.62±0.24)% and the level of serum ALT was (64.10±12.71) U·L－1, γ-GT was (0.80± 0.42) U·L－1, and ALP was (121.20±37.57) U·L－1.In the model control group, the number of hepatoma nodules was (27.4±9.5), the liver weight was (21.38±7.29) g, the ratio of liver/body weight was (5.82±2.31)%, the level of serum ALT was (175.70±48.75) U·L－1, γ-GT was (41.80±15.38) U·L－1, and ALP was (200.50±35.78) U·L－1.In the Pharbitidis Semen group, the number of hepatoma nodules was (8.6±5.3), the liver weight was (13.91±3.55) g, the ratio of liver/body weight was (3.86±0.76)% and the level of serum ALT was (113.10±45.35) U·L－1, γ-GT was (13.40± 6.15) U·L－1, and ALP was (155.80±30.26) U·L－1.The results showed that all indices of Pharbitidis Semen group were higher than those of the normal control group, and lower than those of the model control group (P＜0.01 or P＜0.05). Conclusion Pharbitidis Semen can reduce NDEA-induced injury to the liver cells, and inhibit the overgrowth of the hepatoma.
  • Inhibitory Effect of Chenodeoxycholic Acid-verticinone Ester on Tumor Growth of H22-
       bearing Mice
    ZHOU Qing, ZHANG Jiuliang, WANG Yi
    2015, 34(4): 467-470. https://doi.org/10.3870/yydb.2015.04.012
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    Objective To evaluate the antitumor effects of chenodeoxycholic acid-verticinone ester (CDCA-Ver) on tumor growth and immune system of H22-bearing mice. Methods Antitumor activity against a solid tumor mass was evaluated in Kunming mice.H22 cells were transferred into the abdomen cavity of Kunming mice.H22 cells were inoculated through subcutaneous injection at the right armpit of the mouse to establish a solid tumor model.At 24 h after H22 tumor cells inoculation, 40 tumor-bearing Kunming mice were randomly divided into 4 groups according to random number table (n＝10 each group): model control group, cyclophosphamide (CTX) group, intraperitoneal CDCA-Ver injection group and intravenous CDCA-Ver injection group. In model control group, sterile 0.9% sodium chloride solution (10 mL·kg－1) was intraperitoneally injected once daily. In CTX group and intraperitoneal CDCA-Ver injection group, CTX (20 mg·kg－1) and CDCA-Ver (20 mg·kg－1) was intraperitoneally injected once daily, respectively. In intravenous CDCA-Ver injection group, CDCA-Ver (20 mg·kg－1) was injected through tail vein once daily. CDCA-Ver, CTX and NS were injected into the mice of the experimental groups once daily for 10 days, respectively. The dose volume was 0.1 mL·(10 g)－1 body weight.The positive control drug was cyclophosphamide. Ten mice were treated with 20 mg·kg－1 CDCA-Ver through intravenous injection (i.v.).Ten mice were treated with 20 mg·kg－1 CDCA-Ver through intraperitoneal injection.The thymus and spleen indices and the tumor inhibition rate were assessed, and histopathological examination with haematoxylin and eosin (H&E) staining was carried out to evaluate the antitumor effects of CDCA-Ver. Results CDCA-Ver (ivor ip) suppressed the growth of solid tumor in H22-bearing mice.The inhibition rate was 48.3% at the dose of 20 mg·kg－1 CDCA-Ver (ip).There was no significant difference between CDCA-Ver (ip) and CTX treated group (P＜0.05).Compared with the control, the weight of thymus and spleen of CDCA-Ver (ip) treated group was not obviously changed.But a significant weight loss of thymus and spleen in CTX group was observed, which was attributed to the immune suppression from CTX.The thymus and spleen indices in the CTX-treated mice were significantly lower than those of the control group (P＜0.01).We further conducted histopathological examination to confirm the results.The immune system was not suppressed by CDCA-Ver (ip) in tumor-bearing animals.The low toxicity of CDCA-Ver was an outstanding advantage for the development of newly anticancer drug. Conclusion CDCA-Ver treatment can significantly inhibit tumor growth in mice.
  • Pharmacodynamics Study of Different Lactone Ratios of Hydroxycamptothecin on Mice
       Models of H22 Hepatoma
    WANG Rong, HU Wei, WU Song, FANG Yun
    2015, 34(4): 471-475. https://doi.org/10.3870/yydb.2015.04.013
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    Objective To investigate the anti-tumor effects of hydroxycamptothecin (HCPT) with different lactone ratios on the mice models of H22 hepatoma. Methods Mice models of H22 hepatoma were established.Tumor inhibiting rates of HCPT with different lactone ratios (0%,25%,50%,75%,100%) and the growth status of model mice before and after chemotherapy were observed.Serum biochemical indices were determined to investigate the effects of HCPT with different lactone ratios on hepatic and renal function of the mice. Results Positive control drug and HCPT with different lactone ratios all inhibited the tumor in mice with H22 hepatoma, the inhibition rate was 65.30%, 12.57%, 49.23%, 75.47%, 90.06% and 93.22%, respectively.Compared with the model control group, the living conditions of the mice in HCPT groups were improved.With increasing of lactone ratios, the hepatic injury was alleviated markedly, but the renal injury was aggravated. Conclusion There is a positive correlation between lactone ratios and its anti-tumor effect, and HCPT with 75% lactone can achieve preferable anti-tumor effect with less toxicity as compared with that with 100% lactone ratio.
  • Application of Combined General Anesthesia and Bilateral Thoracic Paravertebral Block
       by Ropivacaine in 20 Cases of Patients Undergoing Off-pump Coronary Artery
       Bypass Surgery
    CHEN Mingbing, ZHANG Yi, JIN Chuangang, WAN Li, LIAO Mingfeng, TAN Juan
    2015, 34(4): 476-479. https://doi.org/10.3870/yydb.2015.04.014
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  • Optimization of the Formula of Konjac Glucomannan-Paeonol Matrix Tablets
    JIE Jing, NIE Shufang, YANG Bo, LAI Xiaojing, LIU Donglin
    2015, 34(4): 502-504. https://doi.org/10.3870/yydb.2015.04.022
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    Objective To optimize the formula of konjac glucomannan-paeonol matrix tablets. Methods The formula of paeonol matrix tablets was optimized by the orthogonal design with the accumulative release rate in vitro as index, with the viscosity of konjac glucomannan (KGM), the amount of KGM and lactose as influence factors. Results The optimized formula was as follows: the viscosity of konjac glucomannan was 20 000 mPa穝, KGM 30%, lactose 20% and the release in vitro fit into the Higuchi equation. Conclusion The formula of the paeonol matrix tablets is reasonable and the tablets have well release effect in vitro.
  • Study on Quality Control Standard of Xinshuaining Mixture
    2015, 34(4): 505-508. https://doi.org/10.3870/yydb.2015.04.023
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    Objective To establish a method for quality control of xinshuaining mixture. Methods The herbal contents including red ginseng,milk veteh,root of red rooted salvia were identified by thin layer chromatography (TLC) and the content of ginsenoside Rb1 in xinshuaining mixture was determined by high performance liquid chromatography (HPLC). Results The herbs can be overtly identified by TLC.Ginsenoside Rb1 had a linear relationship in the range of 1.095－5.475 μg,and the average recovery was 97.3%(RSD 1.98%). Conclusion The method is simple and rapid,and can be used for the quality control of xinshuaining mixture
  • Determination of Anthraquinones in Xinshenyan Capsules by HPLC
    WANG Shudong, QIAN Wenhui, HUANG Lu, LIAO Xin, FANG Li, SU Hua
    2015, 34(4): 509-522. https://doi.org/10.3870/yydb.2015.04.024
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    Objective To establish an HPLC method for the determination of anthraquinones including rhein, emodin and chrysophanol in xinshenyan capsules. Methods Anthraquinones were determined by HPLC with Phenomenex-C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and methanol-1% acetic acid (70:30) as the mobile phase.The flow rate was 1.0 mL·min－1 and the detection wavelength was set at 254 nm. Results The liner range of rhein, emodin and chrysophanol was 4.96－24.80 μg·mL－1 (r＝0.999 6), 6.58－32.91 μg·mL－1 (r＝0.999 9) ,and 15.11－75.55 μg·mL－1 (r＝0.999 9),respectively, and the average recovery was 100.78%, 98.13% and 99.29%, respectively. Conclusion The method is simple and practical, the result is accurate and reliable and it can be used to determine the contents of rhein, emodin and chrysophanol in xinshenyan capsules.
  • Determination of Plasma Protein Binding Rate of Bisoprolol in Human Plasma by Hollow
       Fiber Liquid-phase Microextraction Combined with HPLC
    LIU Yafei,MA Haiying, GONG Xuefei,WANG Baohua
    2015, 34(4): 529-533. https://doi.org/10.3870/yydb.2015.04.032
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    Objective To develop a method for determination of the plasma protein binding rate of bisoprolol in human plasma by high performance liquid chromatography (HPLC) combined with hollow fiber-based liquid-phase microextraction (HF-LPME). Methods Method of liquid phase microextraction was optimized.The concentration of bisoprolol in the reconstitute solution was analyzed by HPLC.The mobile phase consisted of water-methanol-acetonitrile-0.1% phosphoric acid (50:34:6:10).The excitation wavelength was 232 nm and emission wavelength was 300 nm.Through the linear regression equations, the total and free concentrations were obtained, and then the protein binding rate was calculated. Results At low, middle, and high concentration, the protein binding rate of bisoprolol was 31.2%, 32.0% and 31.8%, respectively. Conclusion The proposed method is proven to be simple, fast and reproducible, and is feasible for the determination of plasma protein binding rate of bisoprolol.Bisoprolol moderately binds with plasma protein independent of concentration.
  • Study on the Evaluation Criterion for the Drug Use Rationality of Medical Cases from
       Antimicrobial Clinical Application Monitoring Network of the Ministry of Health
    WANG Chen, LIU Yuanyuan, DING Yufeng, AI Dongyun
    2015, 34(4): 534-537. https://doi.org/10.3870/yydb.2015.04.033
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    Objective To discuss the criteria for the evaluation of rational antibiotic drug use in medical cases (paper edition) of the antimicrobial agent clinical application monitoring network administered by The Ministry of Health. Methods Based on the relevant provisions from The guiding principles for clinical applications of antibacterial drugs and “The notice by the General Office of the Ministry of Health on related questions of clinical application management of antibacterial drugs” (the General Office of the Ministry of Health [2009] NO.38), combined with the actual situations of antibacterial drugs in clinical use, relevant laws, regulations, and guidances, the evaluation items in the feedback form were studied. Results The study, to a certain extent, contributed to the understanding of evaluation criteria on antimicrobial agent use in clinical application monitoring network of The Ministry of Health for monitoring net members by investigating related questions regarding the evaluation criteria, and will make the evaluation results more consistent. Conclusion The discussion of the evaluation criteria can reduce the deviation of the evaluation results and make the evaluation process more standardized.
  • Application Analysis of Total Parenteral Nutrition Use in Neonatal Units
    ZHUO Yubo,XUE Lianfang,WANG Yifei
    2015, 34(4): 538-540. https://doi.org/10.3870/yydb.2015.04.034
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    Objective To investigate how total parenteral nutrition(TPN) is applied in the neonatal units of the first affiliated hospital of Jinan university, provide reference for the standardized use of TPN, formulate appropriate guidelines, and ensure that clinical nutrition is properly and safely used in prenatal patients. Methods Preterm infants with gestational age＜37 weeks and birth weights ＜2 500 g who used TPN in the neonatal units from January 2012 to June 2013 were assigned into two groups, Patients in Group 1 (n＝27) began TPN within 3 d after birth, and patients in Group 2 (n＝28) began TPN 3 d after birth.Body weight and the time to regain birth weight were obtained at discharge,, and biochemical indexes before and after TPN were also evaluated. Results Compared with late TPN, early TPN significantly reduce the time to regain birth weight[(12.5±7.2) d vs (8.9±5.5) d, P＜0.05], and maintained normal blood glucose level (P＜0.05). There were no differences with regards to length of hospitalization, duration of TPN use, growth rate, blood glucose, K+, Ca2+, Cl-, BUN, serum creatinine, BUN and serum bilirubin (all P＞0.05).Both groups can effectively prevent and correct hypoglycemia and hypocalcemia. Conclusion Early TPN can promote growth of preterm infant.The use of TPN can improve hypoglycemia and hypocalcemia without effect on liver and kidney function.Neither group achieved ideal nutritional support outcome and therefore requires further improvement.
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