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  • 01 November 2015 Volume 34 Issue 11
      
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  • Update of the Diagnosis and Management of Diabetes
    GAO Feng, LI Liping
    2015, 34(11): 1411-1416. https://doi.org/10.3870/j.issn.1004-0781.2015.11.001
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    The prevalence rate of diabetes is continuously increasing in China, and diabetes characterizes as high morbidity, low diagnostic rate, low cure rate, and low good control rate, which consequently resultes in the occurrence and progression of various diabetic complications and dramatically decreases the quality of life as well as the survival of diabetic patients.So it is crucial for clinician to screen diabetes early, make the diagnosis early, and start the treatment promptly.This article systemically summarizes the clinical manifestations, diagnostic criteria, typing, pathophysiology, complications, and management of diabetes.
  • Small Intestine Absorption Behavior of Collagen Peptides Microspheres in Rats
    LIANG Ming,TIAN Rende,RAO Rong,ZHANG Ting,DING Liugang,LIU Wei
    2015, 34(11): 1417-1421. https://doi.org/10.3870/j.issn.1004-0781.2015.11.002
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    Objective To investigate small intestinal absorption behavior of oral collagen peptides microspheres in rats. Methods The everted gut sac (EGS) and Single-pass intestinal perfusion (SPIP) model were established in Wistar rats (250－350 g, 5 months).The absorption of collagen peptide in different segments of rat small intestines was detected by BCA method. Results The cumulative transmittance of different particle size of calcium alginate/Chitosan (SA/CTS) microspheres in EGS experiments was that 500 μm microspheres＜200 μm microspheres＜10 μm microspheres (P＜0.01, except the jejunum intestine).The cumulative transmittance of microspheres (10 μm) was: SA/CTS rate (Ka) and permeability rate (Peff) microspheres> SA microspheres(duodenum segment, jejunum segment P＜0.05;ileum segment, P＜0.01).The absorption rate of SA/CTS microspheres was evidently higher than that of SA microspheres in SPIP experiments. Conclusion Small intestine absorption of collagen peptides microspheres is correlated with particle size, and the chitosan in SA/CTS microspheres is beneficial to the absorption of collagen peptide in small intestine segments.
  • Impact of High ABCA3 Expression in Nasopharyngeal Carcinoma Stem Cells on the    Resistance to Cisplatin
    LIN Bihua,CHEN Jing,LU Jiaqi,YU Haibo,ZHANG Xin,ZHOU Keyuan
    2015, 34(11): 1422-1428. https://doi.org/10.3870/j.issn.1004-0781.2015.11.003
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    Objective To investigate the role of ATP-binding cassette (ABC) family on the resistance of nasopharyngeal carcinoma (NPC) stem cells (CSCs) to cisplatin. Methods We compared the differences between the drug extravasation capability of CNE-2 and CNE-2S by using Rhodamine-123 efflux assay.We determined the mRNA and protein expression levels of ABC transport family members, including ABCA3,ABCB1,ABCB5,ABCC1,ABCC2 and ABCG2,after 48 h being treated with 1 μmol·L－1 cisplatin by RT-PC and Western blotting.Rhoamine-123 efflux and apoptosis by cisplatin in two kinds of cells was examined by ABCA3 gene silencing with specific small-interfering RNA. Results The IC50 of cisplatin on CNE-2S was 4.1 fold to that on CNE-2(P＜0.05).For the relative drug effluent activity and Na＋K＋ ATPase activity,CNE-2S was 4.8 fold to CNE-2(P＜0.05),suggested that CNE-2S expressed more ABCA3,ABCB1,ABCC1 and ABCG2 in comparison to CNE-2(P＜0.05).After 48 h treatment with 1 μmol·L－1 cisplatin,ABCA3 specifically highly expressed in CNE-2S (P＜0.05), and knocking down of ABCA3 resulted in reduction of rhodamine-123 efflux and increase of apoptosis. Conclusion The cisplatin resistance of NPC CSCs is associated with enhanced expression of ABCA3,ABCC1 and ABCG2, suppression of ABCA3 could reverse the resistance of NPC CSCs to cisplatin.
  • Effects of Ulinastatin on Renal Expression of Endothelin-1 and Nitric Oxide in Rats    with Toxic Acute Kidney Injury
    SHEN Jianming,DENG Yanyan,YE Tingting,GOU Rong,TIAN Shaojiang,WANG Liping,LI Junfeng
    2015, 34(11): 1429-1432. https://doi.org/10.3870/j.issn.1004-0781.2015.11.004
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    Objective To investigate the effects of ulinastatin on renal expression of endothelin-1 and nitric oxide (NO) in rats with toxic acute kidney injury(AKI). Methods Twenty-four male SD rats were randomly divided into the normal control group,model control group and treatment groups,with 8 rats in each group.Except normal control group, rats were subcutaneously injected with gentamicin (300 mg·kg－1·d－1) for 3 days to establish a model of toxic AKI.Rats in the treatment group were intraperitoneally injected with a 7-day course of ulinastatin (30 000 U·kg－1·d－1) from the fourth day.While the other two groups were injected with 0.9% sodium chloride injection (3 mL·kg－1·d－1).The serum level of creatinine and cystatin-C,urinary concentration of kidney injury molecule-1(Kim-1) and neutrophil gelatinase-associated lipocalin (NGAL),level of endothelin-1 and NO,expression of endothelin-1 mRNA,endothelial nitric oxide synthase (eNOS),induced nitric oxide synthase (iNOS),eNOS mRNA and iNOS mRNA in homogenate of renal tissues in each group were detected on the eleventh day. Results Compared with the normal control group,serum level of creatinine and Cystatin-C,urinary concentration of Kim-1 and NGAL,level of endothelin-1 and NO,expression of endothelin-1 mRNA,iNOS and iNOS mRNA in homogenate of renal tissues were higher in model control group (P＜0.01, respectively), while which were lower in treatment group than those in model control group (P＜0.01, respectively).And no statistical significant difference of eNOS and eNOS mRNA expression in homogenate of renal tissues existed among the three groups. Conclusion Ulinastatin possesses curative role against rat with toxic AKI via down-regulating renal expression of endothelin-1,NO and iNOS.
  • Protective Effects of Memantine on Isofluane-induced Decrease of Proliferation in Neural    Stem Cells
    CHEN Xin,ZHAO Yilin, WANG Wei, ZHANG Jianfang, LI Shiyong,LUO Ailin
    2015, 34(11): 1433-1438. https://doi.org/10.3870/j.issn.1004-0781.2015.11.005
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    Objective To investigate the protective effects of memantine on isofluane-induced decrease of proliferation in neural stem cells (NSCs) and the potential mechanisms in vitro. Methods Neural stem cells were isolated from rat hippocampi (postnatal day 1) and grew in culture.Cultured NSCs were randomly divided into the control (Group Control), Vehicle (Group Vehicle), Isoflurane (Group Iso), Isoflurane＋Memantine (Group Iso＋Mem) and Isoflurane＋Memantine＋LY294002 groups (Group Iso＋Mem＋LY).Proliferation was assessed by cell counting and BrdU incorporation.Western blot was conducted to detect protein expression of phospho-Akt. Results Compared with the control group,BrdU incorporation and phospho-Akt expressions in neural stem cells significantly declined after 2.4% isoflurane exposure for 6 h (P＜0.01).However,isofluane-induced decrease of BrdU incorporation and phospho-Akt expressions was attenuated by the treatments of memantine (P＜0.01)).It was showed that Akt inhibitors LY294002 reversed the protective effects on neural stem cells proliferation by memantine(P＜0.01). Conclusion The results suggest that memantine treatment might attenuate isofluane-induced decrease of proliferation in neural stem cells via Akt signaling pathway.
  • Influence of Celecoxib on Radiosensization of Irradiation-resistant Cell Line CNE-2R of    Nasopharyngeal Carcinoma
    LI Wenwen, SUN Wei, LUO Min, HUANG Yu, ZHANG Linli, HU Guoqing
    2015, 34(11): 1439-1443. https://doi.org/10.3870/j.issn.1004-0781.2015.11.006
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    Objective To investigate the effect of COX-2 inhibitor celecoxib on radiosensitity of irradiation-resistant cell line CNE-2R of nasopharyngeal carcinoma and the potential mechanism. Methods Via exposing to a series of X-ray (2, 4, 6, 8 Gy, 3 times for each dose), radio-resistant cell subline CNE-2R was established from human nasopharyngeal carcinoma cell CNE-2.Radiosensitivity was detected by clone formation assay.CNE-2R and CNE-2 cell lines were exposed to 25, 50, 75 μmol·L－1 celecoxib, respectively.Western blotting was used to detect the protein expression of COX-2.Clone formation assay was performed to measure the survival fraction of CNE-2 and CNE-2R after radiotherapy alone or radiotherapy combined with 30 μmol·L－1 celecoxib treatment.Flow cytometry was used to measure influence of radiotherapy alone or radiotherapy combined with 30 μmol·L-1 celecoxib treatment on cell apoptosis.Number of residual γ-H2AX foci was observed by immunofluorescence assay. Results The colony forming assay demonstrated that the values of SF2, D0, Dq, and N of CNE-2R cell subline [(0.81±0.05), (2.15±0.07) Gy, (2.94±0.08) Gy, (3.91±0.07), respectively] was significant higher than those of CNE-2 cell line [(0.61±0.08), (1.47±0.06) Gy, (1.68±0.10) Gy, (3.13±0.05), respectively].The expression of COX-2 protein was significantly downregulated with increasing celecoxib concentration.Surviving fraction was decreased in both CNE-2 and CNE-2R cell lines after irradiation.After radiotherapy combined with celecoxib, apoptosis rates of CNE-2 and CNE-2R cell lines [(13.10±0.63)%, (5.30±0.75)%] were higher than those of the corresponding control groups [(4.90±0.71)%, (1.82±0.82)%].Celecoxib increased radiosensitivity in nasopharyngeal carcinoma CNE-2R and CNE-2 cell lines.The number of residual γ-H2AX foci after irradiation was increased by celecoxib pretreatment.The difference was statistically significant (P＜0.05). Conclusion Celecoxib can enhance radiosensitivity of radio-resistant cell subline CNE-2R of human nasopharyngeal carcinoma in vitro.
  • Effect of Furin Inhibitor on Growth of Breast Cancer MCF-7 Cell
    DU Xiaojuan,LI Yunfeng,WANG Yan,WANG Hongwei,LI Xuejun
    2015, 34(11): 1444-1447. https://doi.org/10.3870/j.issn.1004-0781.2015.11.007
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    Objective To investigate the role of Furin in breast cancer cell proliferation and provide a theoretical basis for in-depth study of breast cancer. Methods Different concentrations of Furin inhibitor were added in MCF-7 cell culture to test MCF-7 cell proliferation by MTT essay.Hochest 33342 staining was used to detect the morphological change of apoptosic cells.Western blot analysis was applied to measure the level of cell apoptosis associated proteins,such as Caspase-3,Caspase-8 and Caspase-9.The enzyme-linked immunosorbent assay was used for detection the CAT and SOD levels in cell culture. Results MCF-7 cell growth was inhibited by Furin inhibitor in a time and dose dependent manner.The results of Western blot and Hochest 33342 staining indicated that MCF-7 cells were apoptosis after Furin inhibitor treatment.The level of CAT was increased significantly,associated with the level of SOD. Conclusion Furin inhibitor could induce MCF cell apoptosis, thereby inhibit cell proliferation by modulating MCF-7 cell redox state.
  • Intestinal Absorption of Nicorandil in Rats
    FENG Min,DU Juan,LIN Ning
    2015, 34(11): 1448-1450. https://doi.org/10.3870/j.issn.1004-0781.2015.11.008
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    Objective To explore the absorption properties and kinetics of nicorandil from small intestinal segments. Methods The absorption kinetics and permeability of nicorandil under different conditions were investigated by HPLC via the in situ perfusing method in rats. Results There was no significant difference in Ka of nicorandil under different concentrations and different pH.The absorption rate in rats descended in order of jejunum,duodenum,ileum and colon, as (4.69±0.05)×10－2,(3.35±0.04)×10－2,(2.66±0.05)×10－2 and (0.89±0.08)×10－2·h－1. Conclusion The absorption of nicorandil in intestine complies with the passive transport mechanism and first order kinetics.Nicorandil is well absorbed in the whole intestine and can be prepared as delayed release and controlled release forms.
  • Protective Effect of Momordica Saponins Compounds on the Kidney of Type 2    Diabetic Mice
    ZHANG Dexin,CHENG Luping,CHEN Songrui
    2015, 34(11): 1451-1453. https://doi.org/10.3870/j.issn.1004-0781.2015.11.009
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    Objective To study the protective effects of momordica saponins compounds on the kidney of type 2 diabetic mice. Methods The mouse model of type 2 diabetes was induced by feeding high-fat diet for a week,followed by intraperitoneal injection of streptozotocin(STZ)at 150 mg·kg－1.The mice were randomly divided into groups of normal control group(A), model control group(B), rosiglitazone group(C, 0.4 mg·kg－1),the compounds at low-dosage group (D,350 mg·kg－1) and high-dosage group(E,700 mg·kg－1).The levels of fasting blood glucose,UCr,SCr,TGF-β1,mALB,and HbAlc were determined respectively after being treated for 12 weeks. Results Compared with normal control group,the level of fasting blood glucose,SCr,TGF-β1,mALB,and HbAlc in all other groups increased significantly (P＜0.01);Compared with model control group,the level of fasting blood glucose,SCr,TGF-β1,mALB and HbAlc in all treatment groups decreased significantly (P＜0.01) ,the level of UCr increased markedly (P＜0.01). Conclusion The Momordica saponins compounds can inhibit renal injury and exert kidney protective effect on type 2 diabetic mice.
  • Hypoglycemic Effect of n-butyl Alcohol Extraction from Anoectochilus roxburghii
    CHEN Jianfei, ZHANG Xiaochun
    2015, 34(11): 1454-1456. https://doi.org/10.3870/j.issn.1004-0781.2015.11.010
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    Objective To study the hypoglycemic effect and mechanism of n-butyl alcohol extraction from Anoectochilus roxburghii. Methods The total of 12 normal rats were used as normal control group, and 48 diabetic model rats were divided into model control group, and high dose, middle dose and low dose treatment groups with 12 rats in each group,three treatment groups received Anoectochilus roxburghii at dose 600,400,200 mg·kg－1, respectively, normal control group and model control group were administered with equal volume of 0.9% sodium chlorade solution once a day.After 21 days of drug administration, the weights of rats, the levels of blood glucose and insulin were examined. Results Compared with normal control group, the body weight in model control group decreased significantly(P＜0.01), while blood glucose significantly increased (P＜0.01).Compared with model control group, the body weight in Anoectochilus roxburghii groups exhibited no obvious difference,while blood glucose decreased significantly (P＜0.01 or P＜0.05).The level of insulin in high dose group increased remarkably (P＜0.05). Conclusion The n-butyl alcohol extraction from Anoectochilus roxburghii shows markedly hypoglycemic effect,specifically for which at high dose increases the level of insulin significantly,its mechanism may involve with promoting β cells regeneration.
  • Effects of Radix Paeoniae Rubra Extracts on the Endoplasmic Reticulum Stress and Macrophage Recruitment of Diabetic Cardiomyopathy Rats
    ZHENG Yaping, KANG Hongyu
    2015, 34(11): 1458-1461. https://doi.org/10.3870/j.issn.1004-0781.2015.11.012
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    Objective To investigate the protective effects of Radix Paeoniae Rubra(RPR)extracts on reticulum stress and macrophage infiltration on diabetic cardiomyopathy rats and the mechanism. Methods Sprague-Dawley rats were randomly divided into 5 groups: normol control, model control (induced by streptozotocin),RPR extracts at low dose (i.p.15 g·kg－1), middle dose (i.p.30 g·kg－1), and high dose (i.p.60 g·kg－1).The artery intubation was carried out in all rats for detecting cardiac function 10 weeks later.The myocardial structure was detected by masson staining.The level of myocardial tissue glucose regulatory proteins GRP78,caspase-12 and caspase-3 expression were measured by Western blot.The ED-1 protein was tested by immunohistochemical staining. Results Compared with the normal controls, the model rats presented decreased LVSP, ＋dp/dtmax,－dp/dtmax, LVEF, increased expressions of GRP78, caspase-12,caspase-3, ED-1 and the synthesis of collagen fiber(P＜0.05 or P＜0.01).RPR extract inhibited the above indexes of model control rats in a concentration-dependent manner(P＜0.05 or P＜0.01). Conclusion The RPR obviously ameliorates the cardiac function and myocardial fibrosis of diabetic cardiomyopathy rats,which may be associated with suppressing the expression of GRP78,caspase-12,caspase-3 and macrophage infiltration.
  • Effects of Compound Danshen Dripping Pill on Carotid Intima-media Thickness and    Cardiac Function of Patients with Hypothyroidism
    QIAN Wei, WU Hanni
    2015, 34(11): 1462-1465. https://doi.org/10.3870/j.issn.1004-0781.2015.11.013
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    Objective To investigate the effects of compound danshen dripping pill (CDDP) on carotid intima-media thickness (CIMT) and cardiac function in patients newly diagnosed as hypothyroidism. Methods The total of 40 subclinical hypothyroidism patients and 40 hypothyroidism cases was enrolled.The clinical hypothyroidism patients were treated with L-T4 as the conventional treatment, and comprehensive CDDP plus levothyroxine (L-T4) as treated groups (CDDP group) for 6 months.The therapeutic effect was evaluated by combining with data of thyroid function, blood lipid profile and ultrasound.The 40 healthy subjects were chosen as normal control group. Results Compared with normal control group, the levels of TC and LDL-C were higher (P＜0.05) in subclinical hypothyroidism group, which were more obvious in the clinical hypothyroidism group (P＜0.01), CIMT increased markedly (P＜0.05), the left ventricular systolic functions (ejection fraction) and diastolic functions (mitral flow velocity E and A wave ratio) were markedly decreased (P＜0.05) in the clinical hypothyroidism group.Upon treatment, the blood-lipids were improved (P＜0.05), CIMT were reduced (P＜0.05), the left ventricular functions were restored to near normal with the attainment of euthyroidism in both groups of clinical hypothyroidism;the reduction of CIMT and the improvement of the left ventricular functions were faster in CDDP group than that in the conventional treatment group (P＜0.05). Conclusion Disturbance of blood lipid in hypothyroidism patients, CIMT growth and impaired left ventricular function objectively indicate the existence of cardiac and cerebral vascular risk, while CDDP helps improving lipid metabolism, reversing CIMT and cardiac function, reducing cardiovascular risk, blocking or delaying cardiovascular event chains.
  • Forty-nine Cases of Shenmai Injection Administration for Elderly with Orthostatic    Hypotension
    PAN Chunmei,SHEN Dantong,LIN Zhongqiu,XIE Zhiquan
    2015, 34(11): 1472-1475. https://doi.org/10.3870/j.issn.1004-0781.2015.11.015
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    Objective To investigate the effect of Shenmai injection in treating elderly with orthostatic hypotension. Methods The total of 97 cases of patients with orthostatic hypotension were randomly divided into 2 groups, which was injected intravenously with 100 mL Shenmai injection once daily as the treatment group, with 100 mL glucose as the control.The treatment lasted 14 days.The clinical symptom score, the difference of blood pressure, the P-selectin, and the platelet maximum aggregation were measured. Results Compared with the control, Shenmai injection significantly reduced the clinical symptom scores of patients (P＜0.05), lowered the difference of blood pressure for upright and supine significantly (P＜0.05), decreased the platelet maximum aggregation and P-selectin remarkably (P＜0.05). Conclusion Shenmai injection significantly reduces the difference of blood pressure for upright and supine of the elderly with orthostatic hypotension, and inhibits the activities of platelets, thus improves the clinical symptoms.
  • Screening of Film-forming Materials with Dragon Blood Plastics
    SONG Meifang,CHEN Xi,ZHANG Zhonglian, LI Guang, LYU Yana, LI Yihang
    2015, 34(11): 1480-1482. https://doi.org/10.3870/j.issn.1004-0781.2015.11.017
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    Objective To study the film-forming property of film-forming materials in dragon blood ethanol solution, and provide the reference for screening of film-forming materials for dragon blood plastics. Methods PVA124,PVA1788, PVP-K30, EC, MC, PVB were chosen to study the film-forming property of film-forming materials based on solubility, formation time, and adhesion. Results PVA1788 and PVP-K30 mixed in dragon blood ethanol solution in proportion of 4:1 showed good film-forming property, and the membrane was smooth, uniform and easy to tear off.However, PVA124 and PVP-K30 mixed in proportion of 4:1 precipitated in dragon blood ethanol solution.EC, MC and PVB were not easy to tear off the membrane. Conclusion PVA1788 and PVP-K30 mixed in proportion of 4:1 and added with propylene glycol is the optimal choice of film-forming material for dragon blood spray film.
  • Determination of Naringenin and Apigenin in Premna fulva Craib.by HPLC
    YE Yong,HUANG Qiujie,LIU Huagang,CHEN Guangqiang,NONG Yuhong,YANG Liting,ZHONG Jinhua
    2015, 34(11): 1483-1485. https://doi.org/10.3870/j.issn.1004-0781.2015.11.018
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    Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO-SPOLAR C18(250 mm×4.6 mm,5μm) was used as analytical column.The mobile phase consisted of methanol-0.2% phosphoric acid (42:58) with isocratic elution at a flow rate of 1.0 mL·min－1.The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃, the injection volumn was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180～ 3.60 μg (r＝0.999 9) and 0.0052～0.1040 μg (r＝0.999 9), respectively. Conclusion The method is accurate and reliable.It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.
  • Validation of Microbial Limit Test for Levofloxacin Hydrochloride Gel
    WAN Jin,LIN Meng,XIE Wei,WANG Wenqing
    2015, 34(11): 1486-1488. https://doi.org/10.3870/j.issn.1004-0781.2015.11.019
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    Objective To establish a microbial limit test for levofloxacin hydrochloride gel. Methods Ca2＋ was added to neutralize the antibacterial activity of quinolone,and to form the insoluble precipitate by binding with carbomer, therefore destroying the stability of macromolecules in the gel.The microbial limit test for levofloxacin hydrochloride gel was confirmed combining with membrane filtration and plate methods. Results The normal plate counting was used in detecting the mold and yeast colony in the gel, the recovery was more than 70%.Simultaneously neutralization centrifugation and membrane filtration were used in bacterial count,with recovery less than 70%,and controlling bacteria test. Conclusion The method is accurate and practical for microbial limit test of levofloxacin hydrochloride gel,but is risky for bacterial counting and needs further risk assessment.
  • Determination of Related Substances in Lumefantrine by Gradient Elution RP-HPLC
    HE Tingting,ZHENG Jinqi,TAO Qiaofeng,ZHENG Guogang,CEN Yuxin
    2015, 34(11): 1489-1492. https://doi.org/10.3870/j.issn.1004-0781.2015.11.020
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    Objective To establish a gradient RP-HPLC method for determining the related substances in lumefantrine. Methods The HPLC analysis was performed on an Agilent nucleosil 100-5 C18 (4.0 mm×125 mm,5 μm) column;the mobile phase was consisted of solution A,B and C,which included phosphate buffer solutions (consisting sodium hexane sulfonate,pH was adjusted to 2.3)-water-acetonitrile-propanol with different proportion,with a gradient elution at the flow rate of 2.0 mL·min－1, the detection wavelength was 265 nm, and the column temperature was 35 ℃. Results An excellent separation achieved for lumefantrine from its related substances.Lumefantrine had a good linear relationship with the peak area within the range of 0.173 4－0.693 2 μg·mL－1(r＝0.999 6).The limit of determination was 0.06 μg·mL－1. Conclusion The method is sensitive,reproducible and specific for the separation and determination of related substances in lumefantrine.
  • Determination of Levidipine and Related Substance by RP-HPLC
    CHEN Xiaoyu, LI Yinfeng, HUANG Fengyun,ZHOU Bangchang,JIN Chaodong
    2015, 34(11): 1493-1496. https://doi.org/10.3870/j.issn.1004-0781.2015.11.021
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    Objective To develop a reversed phase high performance liquid chromatography (RP-HPLC) method determining the related substances and levidipine. Methods The Welchrom C18 column (250 mm×4.6 mm,5 μm) was used with Octadecylsilane bonded silica as a filler.The mobile phase consisted of methanol-ethanol-water (40:20:40),at the flow rate of 1 mL·min－1 in an isocratic elution,the temperature was at 45 ℃, and the detective wavelength was 240 nm. Results Levidipine could be separated from all impurities and intermediates within the concentration range from151 to 604 ng·mL－1,which had a satisfied linear relationship (r=0.999 9) with a regression equation of Y=0.049 9X＋0.597 9.The LOQ of detection was 31.9 ng·mL－1. Conclusion The developed method is specific,sensitive,easy and fast to operate,which is suitable for detecting levidipine and its related impurities.
  • Establishment of Fingerprint and Mutual Mode of Aqueous Extracts of Eucommia    ulmoides Oliv.from Different Regions by HPLC
    HUANG Xuying, CHEN Ling, CHENG Jing, SONG Hongping
    2015, 34(11): 1497-1500. https://doi.org/10.3870/j.issn.1004-0781.2015.11.022
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    Objective To establish fingerprint and mutual mode of aqueous extracts of Eucommia ulmoides Oliv.from different regions by high performance liquid chromatography (HPLC). Methods The column of SinoChrom ODS-BP (250 mm×4.6 mm, 5 μm) was used.The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution.The flow rate was 1.0 mL·min－1, the detective wavelength was 230 nm, and the column temperature was 25 ℃. Results The fingerprint consisted of 14 common peaks.The overall similarity for ten batches of Eucommia ulmoides Oliv.from different regions was 0.592－0.986.The standard fingerprint of Eucommia ulmoides Oliv.was established by HPLC. Conclusion This fingerprint method is simple and repeatable, and it provides a scientific basis for the quality control of Eucommia ulmoides Oliv.
  • Study on Quality Standard of Huangdi Cataplasm
    HU Junjie,ZHANG Baohui, LYU Qiuxia,WANG Guihong,ZHENG Guohua
    2015, 34(11): 1501-1505. https://doi.org/10.3870/j.issn.1004-0781.2015.11.023
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    Objective To develop the quality standard for evaluating Huangdi cataplasm. Methods Thin layer chromatography (TLC) was used to qualitatively identify Astragalus membranaceus (Fisch.) Bunge,Rheum palmatum Linn,Rhizoma Chuanxiong,Angelica sinensis and Resina Draconis in Huangdi cataplasm.HPLC method was used to determine astragaloside A and loureirin B in Huangdi cataplasm. Results The Astragalus membranaceus (Fisch.) Bunge,Rheum palmatum Linn,Rhizoma Chuanxiong,Angelica sinensis and Resina Draconis were well separated by TLC without interference in the negative control.content of Astragaloside A and loureirin B showed good liner relationships with respective peak area within the range of 6.96－23.2 μg,and 0.072－0.648 μg,with r＝0.999 5,r＝0.999 9, respectively;and the average recovery was 97.18%,and 96.93%,RSD was 1.21% (n＝6),1.53% (n＝6 ), respectively. Conclusion The established qualitative and quantitative detection method is simple, specific, reproducible, accurate and reliable, which can be used for quality control of Huangdi cataplasm.
  • Clinical Outcome of Foley Catheter Balloon Versus Prostaglandins in Labor Induction of    Late Pregnancy: A Meta Analysis
    ADILA Sulidan,HONG Zhidan,LI Jiafu,ZHANG Yuanzhen
    2015, 34(11): 1522-1529. https://doi.org/10.3870/j.issn.1004-0781.2015.11.029
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    Objective To compare the efficacy and safety of Foley catheter (FC) and prostaglandins (PG) for cervical ripening used in labor induction of late pregnant women with unfavorable cervix. Methods Several databases including Cochrane Library,Embase,PubMed,Elsevier,Wanfang Medical Database, CNKI, VIP were searched for collecting the related literatures according to criteria for inclusion and exclusion.Meta analysis was performed by applying software Review Manager 5.2. Results Thirty randomized controlled trials were included.The FC group presented significantly lower risk of excessive uterine contraction [OR=0.38,95%CI(0.19,0.75),P＜0.05] than the PG group.There was no significant difference between the two groups in terms of Cesarean section rate, vaginal delivery rate, Bishop score improvement, duration of induction to delivery, neonatal birth weight, or in neonatal outcome. Conclusion This study shows that FC has good promoting effect of cervical maturity and maternal- neonatal outcomes, and rarely produces excessive uterine contraction.It is safe and equally effective as PG does.The approach is easy to operate and worth to apply in clinical use.
  • Evaluation of the Quality Control System of Clinical Using of Antibiotics
    GUO Yujin,LYU Chuanfeng,JIN Qinghan
    2015, 34(11): 1531-1533. https://doi.org/10.3870/j.issn.1004-0781.2015.11.031
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    Objective To evaluate the quality control standard and check system of clinical using of antibiotics based on the change of the constituent ratio of antibiotics. Methods In this retrospective study, we compared consumption of the ten mostly prescribed antibiotics, constituent ratio of national essential drugs, antibiotics utilization ratio and antibiotics use density between the third quarter of 2012 and that of 2013. Results Compared with 2012, the usage of antibiotics was reduced by 39.91%, and the usage of national essential drugs rose by 71.46%, whereas the consumptions of cefoxitin, aztreonam, cefminox were reduced by 84.56%, 89.99%, and 56.52%, respectively, in 2013.The consumptions of cefazolin, cefuroxime, ceftriaxone rose by 36.39%, 363.50% and 24.06%, respectively.The amount of used antibiotics was reduced by 35.41%, and the application frequency of antibiotics was reduced by 36.62%. Conclusion The establishment, running and implement of the quality control standard and check system of clinical using of antibiotics make constituent ratio of antibiotics used in the first people's hospital of Jining City more reasonable, which will help to promote the rational use of antibiotics in clinic.
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