中国科技论文统计源期刊 中文核心期刊
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖
The diagnosis and treatment of cancer has begun to move to the age of precision medicine with the completion of the human genome project, the implementation of the cancer genome project and the application of cancer targeting drugs. This paper briefly reviewed the history from human genome project to precision medicine Initiative, the achievements of cancer genomics and targeting therapy, and finally put forward the direction of cancer study in the future.
Objective To study the inhibitory effect and mechanism of fluorofenidone on the proliferation of the rat lung fibroblasts induced by TGF-β1. Methods Pulmonary fibroblasts cell were cultured and purified by using differentially adherent method, the third generation was used in the experiment. Cells were pretreated with 1,3,10 mmol·L-1 fluorofenidone, respectively. Pulmonary fibroblasts were identified by immunocytochemistry, and interfered by transient transfection. Cell proliferation was measured by BrdU marking. The mRNA and protein expression of eIF3α and p27 were analyzed by real-time PCR and Western blot. Results Immunocytochemistry staining identification of vimentin was positive in the third generation of pulmonary fibroblasts .TGF-β1 could obviously promote the eIF3α expression and proliferation of pulmonary fibroblasts. EIF3a siRNA was transfected with lipofectamine into cells, which obviously inhibit eIF3α expression induced by TGF-β1, reversed cell proliferation induced by TGF-β1, and could obviously promote p27mRNA expression induced by TGF-β1. Fluorofenidone (3,10 mmol·L-1)could obviously inhibit cell proliferation induced by TGF-β1 and reduce eIF3α expression of pulmonary fibroblasts promoted by TGF-β1. The inhibition rate was 66.7% and 78.8% respectively. Fluorofenidone (3 mmol·L-1,10 mmol·L-1) could also obviously reverse p27 expression inhibited by TGF-β1 ,the reverse rate was 75.5% and 71.3% respectively. Conclusion eIF3α/p27 signal pathway involves in pulmonary fibroblasts proliferation induced by TGF-β1 .Fluorofenidone can inhibit pulmonary fibroblasts proliferation induced by TGF-β1 by inhibiting eIF3α expression, enhancing p27 expression and/or increasing p27 directly.
Objective To investigate the effect of triptolide (TP) on proliferation and apoptosis of cyst-lining epithelial cells with autosomal dominant polycystic kidney disease (ADPKD). Methods Primary cultured cyst-lining epithelial cells were treated with TP at different concentrations for 12 h,24 h,48 h and 72 h, respectively.The proliferation activity of the cells was evaluated by Brdu assay. The cell cycle distribution was determined by flow cytometry. The apoptotic and apoptotic ratio were determined by FITC-AnnexinV binding/PI.The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope. Results TP significantly inhibited the proliferation of cyst-lining epithelial cells and induced apoptosis in a dose- (10-40 ng·mL-1)and time-dependent(12-48 h) manner. Typical ultrastructural changes of apoptotic cells were observed under electron microscope. Conclusion TP significantly inhibited the proliferation of cyst-lining epithelial cells and induced the apoptosis of cyst-lining epithelial cells, thus inhibited cyst forming and delayed cyst developing. The mechanism may involve several targets and pathways.
Objective To study compatibility rule of herb-pair in rhubarb after compatibilities with fructus aurantii Immaturus,Chinese goldthread rhizome,moutan cortex,peach seed and kansui radix stir-baked with vinegar, and to observe its anti-inflammation and acute toxicity. Methods Mice were gavaged by 15,30 g·kg-1 rhubarb and rhubarb, rhubarb frutus aurantii immaturus rhizoma coptidis, radix et rhizoma rhei peony, rhubarb, semen persicae and rhubarb extracts from euphorbia kansui, respectively, in the morning and evening once, for 7 d.The effects of five different compatibility of Rhubarb on acute inflammation were observed in the mouse paw swelling induced by carrageenan. The classical method were used to determine acute toxicity of rhubarb and the contents of five different compatibility of rhubarb. Results Compared with the control group, the contents of five different compatibility of rhubarb with high and low dosage groups(30,15 g·kg-1) could inhibit the paw edema in mice,reduce NO and MDA production and enhanced activity of SOD in mice inflammatory tissue. The LD50 was not determined. Calculated by crude drug content, the MLD of rhubarb compatibilities with fructus aurantii immaturus,Chinese goldthread rhizome,moutan cortex,peach seed and kansui radix stir-baked with vinegar were 145.33,142.30,117.53,103.45,113.09,182.36 g·kg-1 respectively, which were respectively equal to 581,569,470,418,452 ,729 times of people,s daily dried medicinal herb dosage. Conclusion The five different herb-pair have anti-inflammation effects after compatibility of Rhubarb, and it got best effects when rhubarb compatibility with kansui radix stir-baked, the next were rhubarb compatibility with Chinese goldthread rhizome and moutan cortex. It was basic security and low toxicity of herb-pair in rhubarb after compatibilities.
Objective To investigate the effect of Hupo Xiaoshi granules on renal tissue pathological changes and IL-1β, TNF-α and IL-6 in blood of nephrolith model rats. Methods Sixty SD rats were divided into normal control group 10 rats with purified water by intragastric administration(IG),and model group 50 rats with ethylene glycol, calcium and vitamin D3 by IG for 4 weeks to get kidney stones animal models. The successful model rats were randomly divided into model control group, potassium citrate solution group, Hupo Xiaoshi granules low, middle and high dose group (3.335,6.670,13.340 g·kg-1,respectively), 10 rats per group with the corresponding drugs. Four weeks later, rat's renal tissue sections were stained with hematoxylin and eosin, renal tissue changes of calcium stones and pathology were observed.Plasma concentrations of IL-1β, TNF-α and IL-6 were detected by ELISA. Results Compared with the model control group, no renal interstitial hemorrhage and a few or no visible renal tissue calcium crystal deposition in the low, middle and high dose Hupo Xiaoshi granules group. In the normal control group, model control group, potassium citrate solution group,and low, middle and high dose Hupo Xiaoshi granules group,the plasma concentrations of IL-1β were respectively(50.50±13.54),(104.00±24.13),(93.00±20.08),(77.00± 25.21),(71.30 ±26.60),(65.50±26.18)pg·mL-1,respectively;those of IL-6 were(570.10±119.74),(1 040.00±260.15),(861.40±130.04),(740.00±161.31),(680.00 ±239.49),(640.00±238.42)pg·mL-1,respectively;and TNF-α plasma concentrations were(470.30±154.63),(1 010.00±230.94),(820.00±192.12),(830.50±110.01),(720.00 ±140.28),(715.00±173.54) pg·mL-1,respectively. Compared with the model control group, the plasma concentrations of IL-1β, IL-6 ,TNF-α of the low, middle and high dose Hupo Xiaoshi granules group were significantly decreased (P<0.05 or P<0.01). Conclusion Hupo Xiaoshi granules can inhibit renal tissue pathological changes of nephrolith model rats, inhibit calcium crystals stones deposition in renal tissue, and reduce blood levels of inflammatory cytokines.
Objective To evaluate the preclinical safety of 2-methoxyestradiol nanosuspension. Methods The safety of 2-methoxyestradiol nanosuspension for injection was observed through vascular stimulation test of the ear vein on rabbits, hemolytic test using rabbit erythrocytes, active systemic anaphylaxis (ASA) test on guinea pigs and acute toxicity test on mice. Results 2-Methoxyestradiol nanosuspension injection had no irritating effects on vessels, and no haemocytolysis, agglutination and ASA occurred.ASA test showed no allergy symptoms such as piloerection, nose rubbing and dyspnea in guinea pigs 30 min after sensitization.Acute toxicity test revealed that no pathological changes, including black spots and hyperaemia, were visually observed on the heart, liver and lungs after 14 days of intravenous administration. Conclusion 2-Methoxyestradiol nanosuspension injection is relatively safe, with low toxicity, and no hemolytic, anaphylactic and irritating effects.It may be clinically used for injection.
Objective To develop a rapid ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for determination of 11 sedative-hypnotics in human plasma. Methods The plasma samples were extracted with dichloromethane:n-hexane:acetoacetate (5:4:1). The separation was performed on a Waters ACQUITY UPLC HSS T3 column (2.1 mm×100 mm,1.8 μm) using the mobile phase composed of acetonitrile-0.1% ammonium solution at a flow rate of 0.2 mL·min-1 in gradient elution mode. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source in positive mode. Results Good linear relation was obtained over the investigated concentration range, with all correlation coefficients higher than 0.99. The limit of detection was 200 pg·mL-1 for dexzopiclone,and 10-20 pg·mL-1 for other sedative-hypnotics. The intra- and inter-day RSD of 11 sedative-hypnotics were no more than 15.0%. The recoveries were in the range of 72.3%-108.0%, and the matrix effects were approximately 0.86-1.12. Conclusion The method is rapid, sensitive and reliable, and suitable for the simultaneous determination of 11 sedative-hypnotics in human plasma.
Objective To investigate the neuroprotective effects and mechanisms of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase(AICAR) supplement (AMPK activator) in different stages of neonatal rats sufferring from hypoxia-ischemia encephalopathy (HIE). Methods Neonatal rat hypoxia-ischemia brain injury model was employed in this study. A total of 160 neonatal rats were distributed into five groups: sham, model control,AICAR30 min,AICAR24 h and AICAR72 h. The neuroprotective effects of AICAR supplement (30 min, 24 h, 72 h post operation) were compared by cresyl violet staining; Expressions of P-AMPK,AMPK in the brain tissue were measured by Western blotting.Foot-faults method was used to evaluate the long-term prognosis of the rats. Results Compared with the sham group, the survival of rats brain in model control group was significantly decreased [(100.0±0.1)% and (45.3±6.3)%, P<0.05]. AICAR had neuroprotective effects when treated at 30 min and 24 h post operation,while the protective effects disappeared when treated later (72 h post operation) (P>0.05). Compared with the sham group, the expression of P-AMPK significantly increased about three times, while ATP level decreased close to the same. Conclusion Early AICAR treatment can protect hypoxia-ischemia brain injury by increasing AMPK-ATP level.
Objective To investigate protective activity against Aβ25-35-induced cytotoxicity in PC12 cells of different extracts and ursolic acid, which were isolated from pyrola decorata. Methods Aβ25-35-induced cytotoxicity in PC12 cells was established as the model in vitro. The cultured PC12 cells were divided into blank control group, DMSO control group, model control group, different extract groups of pyrola decorate and ursolic acid(UA) group. The different extract groups included ether extract (PE), acetidin extract (AE), n-butanol extract (BE), the water extract (WE), 50% ethanol extract (HEE). MTT assay was used to test the optimum concentration, and the number of viable cells in culture medium was measured by ELISA at 490 nm wavelength. Results The cell viabilities in different extracts groups(PE, AE, BE, WE, HEE) were respectively 89.3%, 77.2%, 79.2%, 75.1% ,74.0% at the concentration of 5.0 mg·mL-1. Moreover, ursolic acid showed the best neuroprotective activity (88.9%) at the concentration of 500 μg·L-1. Compared with model control group, the survival rate of each group was remarkably increased, and the protective activities of PE and UA were more significant among them. Conclusion Different polar extracts of pyrola decorata and isolated ursolic acid have neuroprotective effects on Aβ25-35-induced cytotoxicity in PC12 cells in certain degrees.
Objective To study the effect of night large dose of sodium valproate on epileptiform discharges,and cognitive function of benign childhood epilepsy with centrotemporal spikes. Methods Ninety cases of benign childhood epilepsy with centrotemporal spikes were randomly divided into treatment group and control group (n=45), both groups received regular total doses of sodium valproate therapy 25-30 mg·kg-1·d-1. Patients in the treatment group received treatment that night doses of sodium valproate was double of morning doses. Every night dose was (17.96±1.21) mg·kg-1, and every morning dose was (8.92±0.62) mg·kg-1. The control group received the same dose [(13.37±0.80) mg·kg-1] of sodium alproate therapy twice a day, morning and evening. The epileptiform discharges, cognitive function, therapy effect were compared between two groups. Results Total effective rate of treatment group was 82.22% which were was significantly higher than that of the control group 62.22%(P<0.05);Epileptiform discharges reduction of treatment group was significantly higher than that of the control group (P<0.05), the effective rate of EEG improvement (84.44%)in treatment group was significantly higher than that in the control group 66.67% (P<0.05); FIQ, VIQ, PIQ in treatment group were significantly higher than those in the control group(P<0.05); There were no statistical differences of adverse reactions between two groups. Conclusion Night large dose of sodium valproate treatment can help control epileptic discharge, increase the plasma concentration, improve cognitive function, and therapy effect.
Objective To investigate the relationship between serum 10-hydroxycarbazepine (MHD, the main active metabolite of oxcarbazepine) concentration and oxcarbazepine efficacy and safety, and to optimize rational use of oxcarbazepine. Methods A total of 553 patients were enrolled in a self-controlled and open-label trial to assess the efficacy and safety of oxcarbazepine as monotherapy. The steady state serum MHD trough concentrations after dose were determined by SPE-HPLC. The relationship between MHD level and efficacy were evaluated by logistic regression model and receiver operating characteristic(ROC) curve. Results A total of 498 patients (90.1%) were effective and 404 patients (73.1%) were seizure free after oxcarbazepine monotherapy in this study. The clinical therapeutic range of steady state serum MHD trough concentrations observed in this study was 5-20 mg·L-1, and the corresponding 95% distribution interval of oxcarbazepine daily dose was 9.0-34.5 mg·kg-1·d-1. Logistic regression results indicated a positive correlation of antiepileptic efficacy with serum MHD trough concentration within 0.9-30.0 mg·L-1. The ROC area (95% confidence interval) of MHD trough concentration as the predictor for efficacy was 0.964 (0.938-0.990), which showed accurate predictions. Most of patients whould have good antiepileptic efficacy while the steady-state serum MHD trough concentration remains above 8 mg·L-1. Adverse effects were observed in 104 patients (18.8%) during oxcarbazepine dose escalation phase, and 23 patients (4.2%) during maintenance phase. There were no severe adverse effects associated with oxcarbazepine in this study. Patients with serum MHD concentrations >20 mg·L-1 were at greater risk of developing adverse effects. Conclusion Oxcarbazepine therapeutic efficacy and safety are associated with MHD trough level closely, so it is necessary to monitor MHD concentration.
Objective To assess the efficacy and safety of urinary kallidinogenase combined with sodium ozagrel for cerebral infarction (CI), and provide references for clinical rational drug use. Methods Retrieved from Cochrane library, PubMed, CBM, FMJS, VIP, Wangfang database and CNKI (published until January 2015), randomized controlled trails(RCT)about urinary kallidinogenase combined with sodium ozagrel for treatment of CI were included,then methodological quality were evaluated and statistical analysis of those studies were carried out by Rev Man 5.3.4 software. Results 19 RCTs were included,involving 1 747 patients. Results of Meta-analysis showed that urinary kallidinogenase combined with sodium ozagrel could significantly improve total effective rate[RR=1.18, 95%CI(1.13, 1.23), Z=7.97, P<0.000 01], cure rate[RR=1.42, 95%CI(1.23, 1.64), Z=4.86, P<0.000 1], neurological deficit scores[MD=-4.40, 95%CI(-5.36, -3.43), Z=8.90,P<0.000 01] and activity of daily living scores[MD=19.14, 95%CI(17.39, 20.90), Z=21.36, P<0.000 01]. Conclusion Urinary kallidinogenase combined with sodium ozagrel was effective in the treatment of CI, and no significant adverse reactions were observed. The combination therapy was worthy of clinical application.
Objective To investigate the clinical efficacy of intravitreal ranibizumab injection combined with complex trabeculectomy in treatment of neovascular glaucoma. Methods Totally 38 patients(38 eyes)of neovascular glaucoma were divided into two groups of 19 cases in each randomly. Patients in treatment group were treateded with complex trabeculectomy after intravitreal injection 0.05 mL(0.5 mg) ranibizumab 4-7 days. Patients in control group were treateded with cyclocryotherapy. Visual acuity, intraocular pressure, iris neovascularization and complications were observed. Results The average intraocular pressure of preoperation and postoperation 1 month in treatment group were (6.5±1.7) kPa and (2.1±1.2) kPa.The difference of intraocular pressure before and after operation was significant. The average preoperative and postoperative intraocular pressure in control group were (6.7±1.9) kPa and (2.5±1.2) kPa . The postoprative intraocular pressure of treatment group was lower than that of control group, the difference was statistically significant(P<0.05).Visual acuity and operative reaction after operation in treatment group were superior to those in control group. Conclusion Intravitreal injection of ranibizumab combined with complex trabeculectomy in treatment of neovascular glaucoma is safe and effective and worth in clinical application.
Objective To observe the effects and safety of oxycodone hydrochloride combined with parecoxib sodium in preventing postoperative hyperalgesia induced by remifentanil. Methods Eighty-eight ASA Ⅰ or Ⅱ patients undergoing elective laparoscopic myomectomy surgery were randomly divided into four groups (C,O,P,PO) with 22 cases in each group.Patients in group C,O,P,PO were given with 10 mL saline, 0.1 mg·kg-1 oxycodone hydrochloride, 40 mg parecoxib sodium, 0.1 mg·kg-1 oxycodone hydrochloride and 40 mg parecoxib sodium 30 min before the end of the surgery, respectively. The VAS scores were recorded at the end of surgery 30 min, 1 h, 2 h, 4 h, 8 h, and the cases of agitation, rigor, vomiting and increasing of analgesics were recorded. Results Compared with group C, the VAS scores in group O, P,PO were significantly lower(P<0.05). The VAS scores in group PO were lower than those in group O,P(P<0.05). The cases of agitation and rigor in group PO(0.0%, 5.0%) were less than those in group C (45.0%,23.0%) (P<0.05). The increased doses of analgesics in group PO(0) were less than those in groups C (12), group P (5), and group O (5)(P<0.05). Conclusion Oxycodone hydrochloride combined with parecoxib sodium can effectively prevent postoperative hyperalgesia induced by remifentanil, and reduce the incidence of postoperative agitation.
Objective To determine the optimum harvest time of Scutellaria barbata D.Don with flavonoids contents as the index. Methods The content of total flavonoids in Scutellaria barbata D.Don was determined by ultraviolet-visible spectrophotometry with scutellarin as standard control, and scutellarin and cosmosiin in Scutellaria barbata D.Don were determined by HPLC. Results The contents of total flavonoids and scutellarin in Scutellaria barbata D.Don of different harvest time were complied with the quality standards in the China Pharma Copoeia, and reached the peakin blossom . The contents of total flavonoids, scutellarin and cosmosiin were (45.74±0.95) ,(5.58±0.16 ) and (17.39±0.42) mg·g-1, respectively. Conclusion The Scutellaria Barbata D.Don may be collected at the flowering time with luxuriant foliage.
Objective To establish a headspace GC method for determination of residual organic solvents as methanol,ethanol,dichloromethane and n-hexane in trepibutone. Methods An external standard method was used. A DB-624 capillary column (75 m×0.450 mm,2.55 μm) was used with a FID detector. The injector temperature was 200 ℃ and the detector temperature was 250 ℃.The initial column temperature was 50 ℃,kept for 6 min,then raised to 200 ℃ at a rate of 20 ℃·min-1 and kept for another 12 min.Nitrogen was used as the carrier gas. The flow rate was 3.0 mL·min-1.The headspace vials equilibrium temperature was 80 ℃ and the balance time was 30 min.The injection volume is 1 mL. Results A1l the solvents could be completely separated with good linear relationships.The average recoveries of the four solvents were 100.4%(RSD=0.5%), 100.6%(RSD=0.6%), 99.6%(RSD=0.8%), 98.7%(RSD=0.7%) (n=9),respectively. Conclusion The method is simple and accurate,and can be used in the determination of residual solvents in trepibutone.
Objective To establish a headspace gas chromatography method for determination of nine residual solvents in grape seed extract. Methods The residual solvents in grape seed extract were separated on DB-WAX column (30 m×0.45 mm, 0.85 μm) with an FID detector; the injector temperature and the detector temperature was set at 220 ℃ and 250 ℃, respectively; the chapiter pressure was 27.58 kPa; the containers of headspace injector were in equilibrium for 30 min at 80 ℃; the N,N-dimethylformamide was used as the solvent. Results The detected solvents were well separated. Good linear relationship of the benzene and ethanol was obtained within the range of 0.04-0.3,100-750 μg·mL-1, respectively. Good linear relationships of the hexane, o-xylene, m-xylene, p-xylene, styrene, toluene and 1,2-diethyl benzene solvents were obtained within the range of 0.4-3.0 μg·mL-1(r≥0.995 8), respectively. The average recoveries (n=3) of the nine solvents were 96.35%,97.08%,97.31%,89.93%,92.35%,90.65%,88.56%,93.84%,86.51% and the RSDs were 4.38%,2.16%,3.49%,4.19%,4.80%,4.83%,4.70%,5.00%,4.39%, respectively. Conclusion The established method is simple, rapid and accurate, which can be used for the determination of residual solvents in grape seed extract and can simultaneously provide scientific basic for quality control.
Objective To develop a method for simultaneous determination of three hydrophilic components and two lipophilic components in Radix et Rhizoma Salviae Miltiorrhizae. Methods The RP-HPLC method was performed by using a Welchrom C18 column(250 mm×4.6 mm,5 μm)with a mobile phase of acetonitrile (A)-0.1%phosphoric acid(B). The gradient elution program was as follows:0-15 min,10%→12%A;-35 min,12%→20%A;-45 min,20%→60%A;-65 min,60%→65%A;-80 min,65%→80%A;-90 min,10%A. The flow rate was kept at 1.0 mL·min-1. The detection wavelength was set at 280 nm. The column temperature was 30 ℃. Results A good linearity was obtained over 0.059 5-2.380 0 μg for tanshinol, 0.346 0-13.840 0 μg for rosmarinic acid, 0.656 0-26.240 0 μg for salviamolic acid B, 0.420 0-16.800 0 μg for cryptotanshinone and 0.414 0-16.560 0 μg for tanshinoneIIA, respectively (r=0.999 9). The average recovery rates were between 98.69%-100.91% with RSD less than 1.2%(n=6). Conclusion The method is rapid, accurate, credible and repeatable, and can provide basis for the quality control of Radix et Rhizoma Salviae Miltiorrhiza.
Objective To optimize the matrix prescription and preparation technology of compound pyocutaneous gels. Methods Use the method of L9 (34) orthogonal test, take the Carbopol-940 quantity, pH, glycerol and ethanol as factors,and take viscosity, pH value, appearance properties, temperature resistance as the comprehensive evaluation index, and compare the effects of stewing, centrifugal and ultrasound methods on removing bubbles in the gel. Ultimately determine the optimum preparation process. Results The best matrix prescription of compound pyocutaneous gels is as follows: carbopol-940 1.4%,10% sodium hydroxide solution 3.3%,glycerol 2.3%,ethanol 6%;the best way to remove bubbles is centrifugation,with rotation rate at 3 000 r·min-1for 10 min. Conclusion The selected matrix formulation is simple and feasible, the preparation technology is stable and reliable with good reproducibility, and can be used for the preparation of compound pyocutaneous gel.
The institutional reform of Chinese food and drug administration initiated the structural adjustment proceeding of linkage and optimizing about the responsibilities and institutional integration. Local governments have adopted the synthetically localized or professional regulatory mode. It is necessary to think the general institutional reform trend by adopting the strategic height and new thinking. The paper proposed the analytical framework of Hall three-dimensional structure about the institutional reform of Chinese drug and food administration based on the analysis of the administration system evolution, institutional reform, and so on. Then the author discussed the internal relationship among some factors of the food and drug administration system. It would bring to play the function and performance of the food and drug administration based on three-dimensional system.
