中国科技论文统计源期刊 中文核心期刊
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖
In order to favor the growth and development of the fetus, the maternal-placental-fetal unit is characterized by profound physiologic changes. These adaptations may affect maternal and fetal absorption, distribution, placental transfer, metabolism and excretion of drugs. When evaluating drug use in pregnancy, understanding both pregnancy physiology and the gestation-specific pharmacokinetics of different drugs is necessary to achieve effective treatment and limit maternal and fetal risk. This topic will focus on the pharmacokinetics and safety of drugs during pregnancy in light of changes in maternal physiology and function of placental.
Pharmacogenomics does not only bring the connection of genes, medicines and diseases, but also become a powerful tool for clinical pharmacists. Pharmacogenomics is commonly used in clinical practice, especially in the implementation of genetic-test results for guiding rational use of medicines. The genotyping results of genes can provide good individualized medication guidance for patients, which can be confirmed by clinical use of the clopidogrel and warfarin. As a member of the clinical treatment team, clinical pharmacists should take advantage of pharmaceutical and pharmacogenomics information to promote rational use of medicines.
Objective To estimate the value of nitrofuratoin in uncomplicated urinary tract infection by examining antimicrobial resistance of Escherichia coli (E. coli) strains isolated from the patients. Methods The antimicrobial susceptibility information was collected from two projects: an antimicrobial survey of clinical urine specimens in 20 hospitals in 15 provinces during 2004-2012 and CHINET Antimicrobial Resistance Surveillance Program during 2005-2014. Then the clinical consumption of nitrofuratoin was analyzed according to the data of sample hospitals from 6 cities of Yangtze River system between 2011 and 2015. Results The antimicrobial susceptibility of E. coli strains showed that the resistance rate to cephalosporins and fluoroquinolones were above 50% during the last decade. Meanwhile, the resistance rate to nitrofuratoin was below 10%. Conclusion Nitrofuratoin, which is an old drug in the treatment of urinary tract infection, is famous for its broad-spectrum antimicrobial activity and high sensitivity to ESBLs producing or non-ESBLs producing strains of E. coli. It is efficacious, safe and cost-effective in the treatment to uncomplicated urinary tract infection in women. Therefore, it is highly recommend that rational use of nitrofuratoin in the clinical practice.
Objective To observe the effect of apatinib on the invasion ability and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer ( NSCLC) cell line A549 and H460. Methods Different doses of apatinib were incubated with the A549 and H460 cells, then the effect of apatinib on the invasion of A549 cells was detected by Transwell assay, and the change of cell morphology was observed by optical microscope, and the expression of EMT related proteins were detected by Western blotting. Results The cell viability of A549 and H460 have no change when the concentration of apatinib was under 120 nmol·L-1. Therefore, 60 nmol·L-1 and 120 nmol·L-1 apatinib were used in the further experiments. The invasion of A549 cells was reduced after apatinib incubation for 48 h, with cell morphology changes, and the expression of E-cadherin was enhanced, with the depresion of N-cadherin,Vimentin and Snail, which were related to the progress of EMT. Conclusion Apatinib could induce the MET, and reduce the invasion ability of A549 and H460 cells.
Objective To establish a sensitive method for the determination of rhodamine 123 (Rh123) in rat plasma by high performance liquid chromatography (HPLC). Methods The plasma samples were extracted by acetonitrile, and then separated on a Hypersil BDS C18 colunm (4.6 mm×100 mm, 5 μm) equipped with a guard column kept at 25 ℃. The mobile phase consisted of acetonitrile and phosphate buffer (0.02 mol·L-1,pH4.0) (60:40) and was pumped at a constant rate of 1.0 mL·min-1. The peak was detected using a fluorescence detector set at FLD1A:Ex=485 nm,Em=546 nm. Results In this study, the method was validated for the Rh123 range of 0.1 to 32.0 μg·L-1,and the lower limit of quantitation (LLO Q) was 0.1 μg·L-1. The intra-and inter-day precisions for Rh123 were less than 7%, and the mean recoveries of Rh123 were 87.93%,89.03%,86.11% at low, mid, and high concentrations, respectively. Conclusion A simple, rapid and reproducible HPLC method was developed for the determination of Rh123 in rat plasma, which was an applicable method in modeling and description of the possible pharmacological interactions between the medicines and P-glycolprotein transporter.
Objective This study aimed to investigate the protective effects of ademetionine 1,4-butanedisulfonate (SMT) against radiation injuries. Methods ICR mice were randomly divided into 7 groups, including normal control, irradiation-only, SMT administration-only, low-, medium- and high-dosages (250 ,500, 1 000 mg·kg-1) of SMT pre-irradiation and high-dose of SMT post-irradiation in experimental groups. Blood and immunological experiments, organs index experiment and 30-day’s survival experiment were carried out to observe the protective effects of SMT on peripheral blood and immune system, organ index and the whole body injuries. Results Compared with irradiation-only group (4.23±1.16) ×109·L-1, the number of nucleated cells in bone marrow was (11.20±4.63) ×109·L-1 in the high dose of SMT pre-irradiation. The difference between two groups was significant. Compared with irradiation-only group (19.25±9.36),the colony forming unit-spleen was (39.00±7.57) in the high-dose SMT pre-irradiation group, there was a significant difference between the two groups. The index of liver, spleen, kidney and pancreas were significantly higher than those of the irradiation-only group in SMT administration groups. The survival rate of mice treated with SMT was increased, especially for the high dose group (46% lifted) when compared with irradiation-only group. Conclusion SMT can protect mice from radiation injuries.
Objective To study the absorption features of isoimperatorin in intestine of rat. Methods Establish a single-pass intestinal perfusion model of rat, take phenolsulfonphthalein as a marker for the detection of isoimperatorin concentration from crude extracts of notopterygium, and observe the absorption features of isoimperatorin and its reference substance in intestine of rat. Results The content of isoimperatorin in crude extracts was (0.43±0.02)% (n=5). The absorption parameters of duodenum, jejunum, ileum, colon were (5.28±0.82), (4.47±0.56), (4.17±0.94), (4.32±0.68)×10-5 cm·s-1, respectively. There were no significant differences among them (P>0.05). Compared with the reference substance of isoimperatorin, crude extracts showed better absorption features. Conclusion Isoimperatorin from crude extracts have better characteristics of absorption. This study can provide theoretical basis for design of notopterygium oral formulation.
Objective To investigate the protective effect of Jiejiu oral liquid on acute alcohol-induced gastric mucosal lesions in mice. Methods Sixty Knming's mice were randomly divided into normal control group, model control group, sucralfate group(600 mg·kg-1), the dose of 25.0 g·kg-1 group, the dose of 12.5 g·kg-1 group and the dose of 6.25 g·kg-1 group of the Jiejiu oral liquid (n=10 each).Except for the normal control group, other groups were intragastrically administered with 95% ethanol to create the alcohol-induced acute gastritis model. The effect of pathological histology, injury of gastric mucosa, the mucosal NO, ET-1, TNF-α, IL-6 and PGE2 content of acute alcohol-induced gastric mucosal lesions in mouse were observed. Results The gastric mucosa in model control group appeared obvious bleeding, erosion, and flake ulcer. Compared with the model control group, the acute damage index of the Jiejiu oral liquid therapy groups were obviously reduced (P<0.05 or P<0.01). The content of ET-1,TNF-α and IL-6 in the 25.0 g·kg-1 and the 12.5 g·kg-1 dose group of the Jiejiu oral liquid significantly decreased compared with the model control group (P<0.05 or P<0.01). The content of NO in the 25.0 g·kg-1 group of the Jiejiu oral liquid increased (P<0.05). Jiejiu oral liquid had no significant effect on the content of PGE2 (P>0.05). Histopathologic examination revealed Jiejiu oral liquid can reduce the shedding of gastric mucosa and inflammatory cells infiltration. Conclusion The Chinese herbal mixture Jiejiu oral liquid has a protective effect on acute alcohol-induced gastric mucosal lesions in mice.
Objective To discuss whether Saccharomyces boulardii sachets can improve the eradication rate and reduce some untoward effects of gastrointestinal tract when adding in the Helicobacter pylori (Hp) standard quadruple therapy. Methods 249 cases infected with Hp were randomly divided into control group(124 cases) and treatment group (125 cases). The control group was treated with standard quadruple therapy:colloidal bismuth pectin capsules,100 mg,tid,po;furazolidone tablets,0.1 g,bid,po;amoxil capsule,1 g,bid,po;rabeprazole sodium enteric-coated capsules,20 mg,qd,po.The treatment group was treated with Saccharomyces boulardii sachets(0.25 g,bid,po)besides. Both groups were treated for 4 weeks.Evaluate the effect of Hp eradication rate by using the method of intent of treatment (ITT) analysis and consistent scenario set (PP)analysis. Results According to the ITT analysis,the eradication rate of control group was 89.52%, and that of treatment group was 96.00%. According to the PP analysis,the eradication rate of control group was 90.00%, and the rate of treatment group was 97.39%. Both analysis results showed the eradication rate of Hp in treatment group was significantly higher than control group(P<0.05).As for reducing the untoward effects such as nausea ,inappetence, diarrhea, astriction and erythra, control group was obviously lower than treatment group (P<0.05). Conclusion Compared with standard quadruple therapy only, adding Saccharomyces boulardii sachets can significantly improve the eradication rate of Hp and obviously reduce the untoward effects such as nausea ,inappetence, diarrhea, astriction and erythra.It is worth to be generalized and applied in clinical practice.
Objective To compare the efficacy and safety of Saccharomyces boulardii sachets and bismuth in the treatment of chronic gastritis and duodenal ulcer patients with Helicobacter pylori (Hp)infection. Methods A total of 176 patients with chronic gastritis or duodenal ulcer with Hp infection were enrolled .Patients with chronic gastritis (n=116) were randomly divided into the treatment group (58 cases) and the control group (58 cases) : treatment group received triple therapy(rabeprazole+amoxicillin +levofloxacin) and Saccharomyces boulardii sachets for 14 days; control group received triple therapy and colloidal bismuth pectin for 14 days. Patients with duodenal ulcer (n=60) were ulcer group , given with bismuth-containing quadruple therapy for 14 days,then rabeprazole for 14 days.All patients were tested for 13 C urea breath test four weeks after stopping treatment. Results One hundred and seventy cases completed the trial. Hp eradication rate was 84.5%in treatment group,87.9% in control group ,and 90.0% in ulcer group (analysis by intent of treatment, ITT analysis).The difference between the three groups was not statistically significant(P>0.05).The incidence of adverse events was significantly lower in treatment group (10.3%)than in control group (20.7%)and ulcer group (21.7%)(P<0.05). Conclusion Quadruple therapy with Saccharomyces boulardii sachets can effectively eradicate Hp. It can reduce the occurrence of adverse reactions.
Objective To evaluate clinical effect of rabeprazole combined with teprenone capsules in treatment of gastric ulcer by marking targeting biopsy and leptin. Methods A total of 118 patients with active gastric ulcer confirmed by endoscopy were randomly divided into two groups. Patients in the treatment group (n=60) were given rabeprazole 10 mg, bid ,and teprenone capsules 50 mg, tid. Patients in the control group (n=58) were given rabeprazole 10 mg, bid. Both groups were treated continuously for 56 days. Before and after treatment, 2 groups were labeled with biopsy, the clinical efficacy and the healing rate of two groups were recorded, the quality of healing and the expression of leptin were compared. The level of leptin was tested after treatment. Results After 10 days, the difference of clinical curative effect was not statistically significant (P>0.05). After 56 days, the difference of clinical curative effect was statistically significant (P<0.05); ulcer healing rate (93.33%)in treatment group was higher than that of control group (72.41%); ulcer healing quality (93.33%) in treatment group was higher than that of control group (58.62%); leptin level of treatment group was lower than that of the control group; gastric ulcer recurrence rate (3.8%) in treatment group was lower than that of the control group (24.0%) (all P<0.05). Conclusion Rabeprazole combined with teprenone in the treatment of gastric ulcer is better than rabeprazole. Marking targeting biopsy and leptin can be used to evaluate the healing quality of gastric ulcer more accurately, which can be an evaluation index of the quality of gastric ulcer healing and used as an indicator of the quality of gastric ulcer healing.
Objective To study the effect and adverse reaction of lactulose oral solution on the treatment of postpartum constipation. Methods A total of 120 patients with postpartum constipation were randomly divided into treatment group (n=61) and control group (n=59). The treatment group received 15 mL lactulose oral solution,twice a day.The control group received wheat cellulose particles 3.5 g,twice a day. After the improvement of symptoms, the patient's medication frequency was changed to 1 time a day, the course of treatment was 2 weeks long. The curative effect(including before treatment,period during the treatment,and the first week after finish the whole treatment) and the recurrence of constipation after treatment were observed. Results The frequency of defecation per week in the treatment group was(3.83±1.61)times ,that in the control group was(3.09±1.53)times at the first week after treatment(P<0.05),those were(6.22±1.96)and(5.15±1.71)times respectively in the second week(P<0.01), and (6.43±1.87),(5.29±1.64)times respectively at the first week after treatment(P<0.05).The total recurrence rate in the two groups were 4.92% and 10.17% respectively(P>0.05) after follow-up to 60 d. The degree of drug acceptance in two groups was 100.00% and 76.27% respectively(P<0.01).There was no obvious adverse reaction in both two groups. Conclusion The effect of lactulose oral solution on the treatment of postpartum constipation is definite and with low recurrence rate. Lactulose oral solution has a good taste, easily to be taken and to be accepted, it is worthy of clinical application.
Objective To study the real world of bicyclol tablets in the treatment of viral hepatitis and its influence on platelets. Methods The hospital information system ( HIS) was selected from 18 Triple-A hospitals in China on patients with bicyclol tablets information as exposed group (2 690 cases), patients with unused bicyclol tablet (2 690 cases) as an exposed group, an exception occurs of platelet as an index of the ending. Results The incidence of abnormal platelet difference in two groups before the treatment were not statistically significant. The incidence of abnormal platelet reduce between two groups have statistical significance, can be thought of exposure of abnormal lower rate (11.75%)lower than that of non exposed group(15.17%) (P<0.01). Conclusion Bicyclol piece have signficantly curative effect on the treatment of viral hepatitis. It shows more safety, and less impact on platelets.It is worth to be used in clinical practice.
Objective To study the influence of penehyclidine hydrochloride(PHC)on inflammatory factors in patients with acute lung injury(ALI). Methods Total of 96 patients with ALI were randomly divided into treatment group(48 cases)and control group(48 cases). Patients in the treatment group were given conventional therapy, plus penehyclidine hydrochloride injection 1 mg,im,q12 h or q8h,the first dose could be doubled,at least 2 days of continuous application; Patients in the control group were received conventional therapy. Tumor necrosis factor α (TNF-α), interleukin -6 (IL-6), high sensitive C reactive protein (hs-CRP), calcitonin (PCT), oxygenation index (PaO2/FiO2), lung injury score (LIS) and acute physiology and chronic health status score (APACHE II) of patients on each time point were compared between the two groups before and after treatment,and mechanical ventilation time, intensive care (ICU) treatment time, acute respiratory distress syndrome (ARDS) and ICU mortality were recorded. Results On each time point of treatment,TNF-α, IL-6, hs-CRP, PCT, LIS, APACHE II in treatment group were significantly lower than that of control group (P<0.05), PaO2/FiO2 was significantly higher than that of control group(P<0.01). The mechanical ventilation time,ICU treatment time in treatment group were shorter than that of control group(all P<0.05),The incidence of ARDS in treatment group and control group was 20.8% and 39.6%, respectively(P<0.05);ICU mortality rate was 12.5% and 31.2%,respectively(P<0.05). Conclusion Penehyclidine hydrochloride can effectively improve the oxygen metabolism in acute lung injury, regulate the pulmonary inflammatory response and improve the prognosis of patients.
This paper aimed to introduce and review the antioxidant and material base of selaginella medicinal plants. On the basis of the systematic identification of selaginella medicinal plants, we found that the ethyl acetate extract and flavonoids could inhibit the activity of XOD, LOX or/and COX-2 to some extent. Fourteen biflavonoids, one flavonoid and five flavonoid glycosides have been isolated from three resource plants, among them five compounds were firstly reported and fifteen compounds were firstly isolated in selaginella plants. Amentoflavone (AME), robustaflavone and robustaflavone-4'-dimethylether are three common characteristic constituents of selaginella plants, which can act as marks of common active ingredients and be applied to evaluate the quality of selaginella. Further studies found that some plants had stronger anti-Coxsackie virus and anti-tumor effects, and their mechanisms were associated with inhibiting multiple oxidase and mitigating harm of free radicals due to whole function.
Objective To study the prescription and preparation technology of breviscapine microemulsion for parenteral injection, and to evaluate its quality. Methods The prescription was selected and optimized through single-factor test and the pseudo-ternary phase diagram method. The preparation technology was investigated, and the particle diameter, drug content, encapsulation efficiency and haemolyticus were evaluated. Results The prescription composition of breviscapine microemulsion was soybean oil:phospholipid:HS15:PEG400:water=1:0.1:0.55:0.55:6.64 (m/m), with the drug content of 4.01 mg·mL-1, the acquired breviscapine microemulsion exhibited light yellow, uniform and transparent, with average particle diameter of 92.1 nm and encapsulation efficiency of 96.8%. The compatibility test showed no drug precipitation and the preparation was no hemolytic crisis. Conclusion The preparation of breviscapine microemulsion injection is correspond to the main index of parenteral injection.
Objective To establish a quality evaluation method for determination of paris saponinⅠ,Ⅱ,Ⅵ,Ⅶ in paridis rhizhma by quantitative analysis of multi-components with single-marker (QAMS). Methods An HPLC method and a Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm)were used. The mobile phase was acetonitrile-water (48:52) at a flow rate of 1.0 mL·min-1. The detection wavelength was 203 nm and column temperature was 25 ℃. Parissaponin Ⅶ was used as the internal reference substance. The relative correlation factors of parissaponinⅠ,Ⅱ,Ⅵ were calculated by standard curve method and QAMS. Results The QAMS method could be used for determination of four saponin components at the same time without significant difference as compared with the results of standard curve method (RSD<2.0%). Conclusion QAMS method is simple and reproducible, which can provide a reference for quality standard revision for paridis rhizhma.
Objective To optimize the particle size of Huoxue powder by contents comparison of emodin, phellodendrine, berberine and jatrorrhizine before and after permeabilized skin absorption of Huoxue powder in different particle size of 0.150,0.075,0.048,0.038 mm. Methods The contents of emodin, phellodendrine, berberine and jatrorrhizine in transparent absorbent fluid of Huoxue power in different particle size within 24 hours were measured by ultra performance liquid chromatography tandem mass spectrometry, and optimize its particle size by contents comparison of the effective components. Results The contents of the active components in Huoxue power with the particle size of 0.075 mm were high before and after percutaneous absorption. Conclusion Particle size of 0.075 mm is best for Huoxue powder.
Objective To evaluate the correlation and difference of reversed phase high performance liquid chromatography (RP-HPLC) and fluorescence polarization immunoassay (FPIA) on determining serum concentration of carbamazepine. Methods Fifty serum samples were collected, both RP-HPLC and FPIA methods were employed to determine the concentration of carbamazepine.The results were analyzed by paired t test, Bland-Altman and Deming regression methods, respectively. Results The results of measuring 50 samples by the two methods showed that FPIA datas were significantly higher than RP-HPLC datas,and there was statistically significant difference(P<0.05) and poorer consistency between two methods; There was good correlation between carbamazepine concentrations determined by the two methods. Deming regression equation was CFPIA=1.195 3 CRP-HPLC-0.144 0, and Pearson correlation coefficient was 0.968 5. Conclusion Clinicians should pay more attention to the difference of carbamazepine concentration determination by different methods when carbamazepine individualized dosage regimen was adjusted according to therapeutic drug monitoring.
Objective To investigate the risk factors for nosocomial infections and analyze pathogens drug resistance in patients with craniocerebral injury so as to provide scientific basis for clinical precaution and treatment. Methods A retrospective survey was conducted to collect the clinical data of 480 patients with craniocerebral injury including the gender, age and therapeutic process of patients by medical history, for analyzing the related factors causing nosocomial infections via SPSS 18.0 software. Then, the results of bacterial culture and drug sensitivity test were recognized to analyze drug resistance of pathogenic bacteria. Results The nosocomial infections occurred in 100 of 480 patients with craniocerebral injury, with an infection rate of 20.83%. The univariate analysis indicated that the risk of nosocomial infections was associated with age, tracheal tube, deep vein catheterization, the length of tracheotomy, the length of hospital stay, diabetic mellitus and hemoglobin ≤110 g·L-1 (P<0.05). Totally 191 strains of pathogens have been isolated, including 118 (61.8%) strains of gram-negative bacteria, 46 (24.1%) strains of gram-positive bacteria and 27 (14.1%) strains of fungi. The Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli were dominant among the gram-negative bacteria, and the drug resistance rates of them to cefazolin and cefotaxime were high; The Staphylococcus aureus, Enterococcus faecalis and Staphylococcus were the major species of the gram-positive bacteria, which were insensitive to penicillin and clindamycin; the Candida albicans was the major species of the fungi. Conclusion The incidence of nosocomial infections is high in the patients with craniocerebral injury, which is accociated with many risk factors. Therefore, it is necessary to strengthen the control of the risk factors so as to effectively reduce the incidence of nosocomial infections.
