中国科技论文统计源期刊 中文核心期刊
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖
Pyrrolizidine alkaloids(PAs) are widely dispersed in the plants,and approximately 120 PAs could induce liver toxicity.Compositae,Boraginaceae,Leguminosae and several families in the plants contain PAs.In recent years,there are many HPAs-induced liver injuries caused by the use of Gynura segetum(Lour.) Merr in China and these events caused the extensive concern.By reviewing the relevant literatures,the pathogenesis,mechanism,diagnosis and treatment,risk factor as well as risk management was studied,in order to provide reference for clinical application and risk management of the HPAs-containing herbs such as Gynura segetum(Lour.) Merr.
Objective To study the effect of curcumin (Cur) analogues H8 (H8) on inflammation factors in mice liver. Methods Forty db/db mice were divided into five groups,including model control group,rosiglitazone group (5 mg·kg-1),Cur group (5 mg·kg-1),H8-L group (5 mg·kg-1) and H8-H group (10 mg·kg-1) (n=8),and normal control group (n=8 db/m mice). Normal control group and model control group were intragastrically administered with 1% CMC-Na,the treated group mice were fed with rosiglitazone,curcumin and H8 dissolve in 1% CMC-Na for 8 weeks. The protein expression levels of IL-6,IL-17 and TNF-α were assayed by immunohistochemical staining and the expression levels of PI3K and p-AKT were detected by Western blotting. Results Compared with model control group,the protein levels of IL-6,IL-17 and TNF-α were decreased in the treatment groups,especially for H8-H group,and the expression of PI3K and p-AKT were significantly increased in the treatment groups. Conclusion It has been established that H8 can decrease inflammatory protein in liver of db/db mice. H8 can promote PI3K/AKT signaling pathways and can improve the db/db mice glucose metabolic disorder.
Objective To observe the effects of Chaige Shubiao granules on the secretion of inflammatory factors in lipopolysaccharide (LPS)-induced murine RAW264.7 macrophages. Methods The inflammation model was established using LPS-induced RAW264.7 cells in vitro. The cytotoxicity of Chaige Shubiao granules was measured by MTT method. Griess assay was performed to measure the released nitric oxide (NO) in the culture supernatant,and ELISA method was applied to detect the contents of TNF-α and IL-1β. Results Chaige Shubiao granules exhibited no significant effect on RAW264.7 cell viability at 12.5-50.0 μg·mL-1 concentrations (P>0.05). The secretion of NO,TNF-α and IL-1β from LPS-induced RAW264.7 cells were significantly increased,respectively (P<0.01). Chaige Shubiao granules (25.0,50.0 μg·mL-1) could significantly inhibit NO release in RAW264.7 cells stimulated by LPS (P<0.01). Chaige Shubiao granules (12.5,25.0,50.0 μg·mL-1) could also inhibit TNF-α and IL-1β secretion in LPS stimulated RAW264.7 cells in a concentration-dependent manner. Conclusion Chaige Shubiao granules exert anti-inflammtory effects in LPS-induced RAW264.7 cells,which might be mediated by decreasing the inflammatory factors such as NO,TNF-α and IL-1β.
Objective To observe the in vitro activity of iodophor against planktonic Staphylococcus aureus and the Staphylococus aureus biofilm on surface of titanium plate,and provide reference for further animal experiment and clinical research. Methods The quantitative suspension tests were carried out to observe the antibacterial activity of iodophor against Staphylococcus aureus at planktonic state. 24-h old Staphylococus aureus biofilm was establishd on the surface of titanium plate and detected by the confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Colony forming unit (CFU) assays were performed to quantify the viable bactertia in the biofilm after the plates were treated with indicated iodophor. Results In laboratory conditions,all planktonic Staphylococcus aureus were killed in 30 s with 0.1%,0.2%,0.3%,0.4% and 0.5% iodophor (available iodine concentration). After immersed by iodophor for 5 min,with the continuous increase of iodophor concentration,the CFU viable count on the titanium alloy disk was declining. After immersed by 0.5% iodophor for 5 min,most of the bacteria in the biofilm were killed,but not all of them. Extending the immersing time to 10 min,the CFU became zero. Conclusion The iodophor has antibacterial activity against not only the planktonic Staphylococcus aureus,but also the 24 h Staphylococus aureus biofilm in vitro. And the antibacterial activity enhanced with the increasing of iodophor concentration and effecting time.
Objective To investigate the effect of Shensong Yangxin (SSYX) puvis on cardiac fibrosis and the underlying mechanisms. Methods Aortic banding (AB) was performed to induce cardiac fibrosis. Forty-five C57/BL6 mice were randomly assigned into three groups:vehicle sham group,vehicle AB group,and the SSYX AB group (n=15 each gorup). Microvascular density (MVD) was evaluated by immunohistochemistry method. The expression levels of CD31,CD34,α smooth muscle actin (α-SMA) and vimentin were detected by immunofluorescence staining and western blotting evaluated the extent of endothelial to mesenchymal transition (EndMT). Results The capillary density increased (P<0.05),the protein expression of endothelial markers increased significantly,while the protein expression of interstitial cell markers (alpha-SMA and vimentin) decreased significantly (P<0.05) after SSYX treatment. Conclusion SSYX may attenuate cardiac fibrosis via regulating EndMT which is probably mediated by blockade of TGF-β/Smad pathway.
Objective To evaluate the efficacy of vancomycin used to treat severe bacterial infections of neonates and to provide a reference for clinical drug application. Methods In total,102 neonates treated with vancomycin were retrospectively analyzed. Disease distribution,results of therapeutic drug monitoring,bio-chemical examination,etiological examination and drug sensitive test were statistically analyzed. PK model established by Mehrotra was applied to calculate the ratio of drug concentration-area under the curve in 24 h and minimal inhibitory concentration (AUC24/MIC). Results Among the 102 cases,patients diagnosed with neonatal sepsis accounted for the highest percentage. And 36.27% of the patients had a trough concentration of vancomycin within the range of 10-20 μg·mL-1 in the first serum concentration test. After a treatment of vancomycin,clinical biomarker values of CRP,Cr,Urea,T-BiL,ALT and AST were decreased significantly. Totally,73 cases of Gram-positive bacteria were isolated and cultured and AUC24/MIC values were calculated. Most of them were much less than 400 (which is recommended by the expert consensus document). Conclusion Vancomycin has a definite efficacy in treating severe infections caused by Gram-positive bacteria in neonates. However,therapeutic drug monitoring should be applied in order to enhance security and effectiveness.
Objective To analyze the clinical application of vancomycin in pediatrics and promote its medication safety and rationality. Methods The retrospective investigation and analysis methods were applied in this work. In total,134 cases of hospitalized children medically treated with vancomycin during September 1,2016 and August 31,2017 were collected and analyzed. Results A total of 192 measurements were performed for vancomycin plasma concentrations in 134 patients,of which 89 were male (66.42%),of wich 45 were female (33.58%) and 56.72% were from the department of hematology. Vancomycin was administrated in 92.54% of the children with an initial dose of 10-20 mg·kg-1. The treatment efficiency was 88.06%. The major adverse reactions were rashes with an incidence rate of 2.99%. Thirty-eight strains of Gram-positive bacteria were detected in etiological examination. Vancomycin-resistant enterococci were not found yet. Of the 192 measurements,66.67% of the trough concentrations were found under the lower limit of recommended concentration range. Conclusion The use of vancomycin in this hospital is relatively reasonable,yet it is necessary to pay attention to the trough concentration. Meanwhile,further study is still needed to investigate the factors affecting plasma concentration of vancomycin,in order to improve the implement of individualized treatment.
Objective To discuss the role of clinical pharmacists in drug therapy. Methods A clinical case of pediatric acute lymphoblastic leukemia combined with pulmonary,ocular and intracranial multisystem mucor infection was reported. By analyzing the condition,exploring guidelines and literature,the clinical pharmacist recommended the antifungal treatment regimen of amphoterycin B liposomes combined with amphoterycin B sheath injection for multisystem fungal infection. Results The child’s clinical symptoms were controlled effectively and the chemotherapy regimen was completed successfully. Conclusion By participating in clinical practice,the clinical pharmacists can assist doctors in making a rational drug treatment plan and improving the level of drug therapy.
Objective To investigate and analyze total parenteral nutrition (TPN) prescriptions in the neonatal units,and to provide further reference for standardized use and ensure the safety and the effectiveness of the neonatal parenteral nutrition support. Methods A total of 1843 newborn parenteral nutrition prescriptions in the pharmacy intravenous admixture services (PIVAS) of the affiliated hospital of Qingdao university were selected from January 2017 to June 2017. Disease category and prescription were statistically analyzed to explore the influence factors on the rationality and stability of TPN. Results The diseases in 1843 prescriptions conformed to indications for neonatal parenteral nutrition. The time of TPN support was 5-40 days (an average of 15 days). Glucose concentration was reasonably controlled. Totally,428 cases (23.22%) had irrational proportion of amino acid,184 cases (9.98%) had irrational amino acid concentration,276 cases (14.98%) unreasonable thermal nitrogen ratio,and 316 cases (17.15%) unreasonable glycolipid ratio. Conclusion There are some problems in component ratio of neonatal TPN prescriptions. Pharmacists should strictly check the prescription to ensure the safety and effectiveness of clinical neonatal TPN.
Objective To investigate the effect of azithromycin combined with montelukast on immunoglobulin,T lymphocyte subsets and lung function in children with mycoplasma pneumonia. Methods Totally 112 children were randomly divided into two groups,the treatment group and the control group (n=56). The control group was treated with azithromycin,and the treatment group was treated with 4-5 mg of montelukast sodium on the basis of the treatment in the control group for continuous 14 days,po,qd. The immunoglobulin,T lymphocyte subsets and lung function indexes of the two groups were detected and compared. Results After treatment,the levels of IgA,IgG,IgM and C$D^{+}_{8}$ in the two groups were significantly decreased,and the levels of IgA,IgG,IgM and C$D^{+}_{8}$ in the treatment group were lower than those in the control group,and the differences were statistically significant (P<0.05). The C$D^{+}_{3}$,C$D^{+}_{4}$,C$D^{+}_{8}$,C$D^{+}_{4}$/C$D^{+}_{8}$ in two groups were elevated,and C$D^{+}_{3}$,C$D^{+}_{4}$,C$D^{+}_{8}$,C$D^{+}_{4}$/C$D^{+}_{8}$ levels of the treatment group were higher than those of the control group,with statistically significant differences (P<0.05); the FEV1,FVC,PEF of two groups were elevated,and FEV1,FVC,PEF levels of the treatment group were higher than those of the control group,with statistically significant differences (P<0.05). The total effective rates of the treatment group and control group were 94.64% and 80.36% respectively,with statistically significant differences (P<0.05). Conclusion Azithromycin combined with montelukast can effectively improve immune function and lung function in children with mycoplasma pneumonia,and its clinical efficacy is good.
Objective To study the influence of entropy index method based monitoring management on sedative drug dosage for patients with severe mechanical ventilation. Methods Totally,61 cases patients with severe mechanical ventilation from intensive care unit (ICU) were selected,the patients were divided into treatment group (n=31) and control group (n=30) by using the random number table method. All the patients were sedated with dexmedetomidine during mechanical ventilation. The control group were given Ramsay sedation score system based monitoring management,and the treatment group were given entropy index method based monitoring management. Results The sedative drug dosage of treatment group and control group were (13.25±3.21),(43.15±5.61) μg·h-1,the withdrawal awakening time were (3.26±1.54),(9.06±1.87) min,the mechanical ventilation time were (4.56±1.63),(6.59±2.08) d (all P<0.01). Heart rate (HR) and mean arterial pressure (MAP) level between the two group were not statistically significantly different (P>0.05). The two groups did not have unplanned extubation events and adverse reactions such as drug allergy. Conclusion Entropy index method based monitoring management can reduce sedative drug dosage for patients with severe mechanical ventilation,promote body recovery. It is effective and safe.
Objective To investigate the effect of folic acid combined with vitamin B12 in the treatment of mild cognitive impairment (MCI) in patients with hyperhomocystinemia (HHcy). Methods In total,100 cases of MCI with HHcy were randomly divided into treatment group and control group (50 cases in each group),and healthy group also had 50 cases. The treatment group was given folic acid 10 mg·d-1 and vitamin B12 tablet 500 μg·d-1; the control group was given the consolation medicine orally; no drug intervention was given in the health group. They were observed for 24 weeks. The levels of plasma Hcy,folic acid and vitamin B12 were measured in the three groups before and after the 24 weeks of treatment. The simple mental state Checklist (MMSE) and the Montreal cognitive assessment scale (MoCA) were evaluated. Results After the treatment,the plasma Hcy in the treatment group was significantly decreased as compared with that before the treatment [(17.96±8.72) vs. (27.30±8.78) μmol·L-1],folic acid increased [(17.23±6.70) vs. (8.59±5.97) ng·mL-1],vitamin B12 elevated [(335.03±78.35) vs. (234.87±70.87) pmol·L-1] (all P<0.01). There was no obvious change in the score of MMSE and MoCA. There was no significant change in plasma Hcy,folic acid,vitamin B12 and MMSE scores in the control group,and the score of MoCA scale decreased (P<0.01). Conclusion Folic acid combined with vitamin B12 can effectively reduce plasma Hcy in patients with HHcy accompanied by MCI and delay cognitive decline in MCI patients.
Objective To compare the hemostatic effect of hemocoagulase vs. tranexamic acid in patients with craniocerebral trauma surgery. Methods In total,100 patients with craniocerebral trauma surgery were randomly divided into group A and group B (50 cases in each group). Group A was intravenously injected with tranexamic acid injection 0.25g+0.9% sodium chloride solution 250 mL from operation beginning to operation ending. Group B was intramuscularly injected with hemocoagulase 1U+0.9% sodium chloride solution 10 mL 30 min before the surgery,and intravenously injected with hemocoagulase 1 U+0.9% sodium chloride solution 250 mL from operation beginning to operation ending. Results The intraoperatve blood loss of group A and group B was (968.02±102.96),(726.97±81.42) mL,blood transfusion volume (735.12±74.24),(575.72±60.67) mL,drainage 24 h after the operation was (182.45±23.54),(150.22±18.47) mL. Re-bleeding rate was 16.00% and 2.00% (all P<0.05). According to the Glasgow Prognosis Score,good prognosis rate of group B (60.00%) was significantly higher than that of group A (40.00%) (P<0.05). The preoperative and postoperative prothrombin time (PT),activated partial thromboplastin time (APTT) and adverse reaction rates of the two groups were almost the same,and there was no thrombosis disease (P>0.05). Conclusion Compared with tranexamic acid,the hemostatic effect of hemocoagulase is better in patients with craniocerebral trauma operations,and it is helpful to avoid postoperative re-bleeding and improve the prognosis of patients.
Objective To prepare a new venlafaxine maleate for extending release and decreasing the side effects of the drug. Methods Liquid assisted grinding and solution stirring method have been used to prepare the venlafaxine maleate. Single crystal X-ray diffraction analysis (SXRD),powder X-ray diffraction analysis (PXRD),differential scanning calorimetry analysis (DSC),and thermogravimetric analysis (TGA) were used to characterize venlafaxine maleate. The stability of venlafaxine maleate was also determined under high temperature (60±1) ℃,high humidity (90±5)%; and strong illuminance (4500±500) lx. Results The product was prepared with venlafaxine and maleate at molar ratio 1:1. Venlafaxine maleate was stable under three conditions. Conclusion The crystal structure,preparation and characterization spectrum of venlafaxine maleate is reported in this study,which can provide basic data and technical references for further development and research of venlafaxine maleate.
Objective To optimize the extraction technology of total flavonoids from Humulus lupulus L.. Methods Conditions for the extraction were studied by orthogonal experimental design guided by the yield rate of dried water decocting rates,the extraction ratio of total flavonoids and xanthohumol in the extract.The data were analyzed with the comprehensive evaluation of multi-targets for optimizing the extraction condition. Results The optimal extraction condition was as follows:12-fold ethanol solution (70%) was served as extraction solvent, the extracting frequency was 3 times and extract 2 h each time. Conclusion The extracting technology is convenient and rapid for the extraction of total flavonoids from Humulus lupulus L.
Objective Photothermal reagent (IR780) is insoluble in water,which leads to low cellular uptake efficiency and limits the effect of photothermal therapy. This study aims to solve this problem. Methods The mesoporous silica nanoparticles were synthesized by Cetyltrimethyl Ammonium Bromide (CTAB) template method and then loaded with IR780. Finally they ware incubated with tumor cells. IR780 entering into cells was observed. Photothermal therapy efficacy was observed. Results Water solubility of IR780 was significantly increased after it was modified with mesoporous silica nano particles. And the uptake of IR780 by tumor cells was significantly increased,then the effect of photothermal killing was also significantly enhanced. Conclusion The mesoporous silica nano-drug delivery system can significantly improve the uptake efficiency of IR780 and the photothermal therapy on tumor cells.
Objective To establish a quality standard of Shuangbei Capsules. Methods Fritillaria thunbergii,Fritillaria thunbergii Miq,Forsythia in Shuangbei capsules were identified by thin-layer chromatography (TLC). The content of tubeimoside I was determined by high performance liquid chromatography (HPLC). Results The characteristic identification by TLC was distinct and specific. There was a good liner relationship with contents of tubeimoside Ⅰ in the ranges of 0.182-0.912 mg·mL-1 with good correlation coefficient r=0.9997. The average recovery was 99.31% and RSD was 0.49%. Conclusion The established methods are simple,accurate,exclusive,reproducible and suitable for the quality control of Shuangbei capsules.
Objective To establish high performance liquid chromatography (HPLC) fingerprint and control the quality of Yuantong mixture. Methods HPLC was applied to analyze the constituents of Yuantong mixture. The chromatography conditions were:mobile phase of acetonitrile-0.1% phosphoric acid,gradient elution,flow rate 1.0 mL·min-1,detection wavelength 280 nm,column temperature 30 ℃,injection volume 10 μL and the running time of 70 min,using the National Pharmacopoeia Commission "HPLC Chromatographic Fingerprint Similarity Evaluation System" (2010 Edition). Results The HPLC fingerprints of Yuantong mixture were established,and 10 common characteristic peaks were demonstrated. The similarity of HPLC fingerprint was 0.9 for 10 batches of samples. Conclusion The method of HPLC fingerprint was simple and reproducible,and could reflect the quality of Yuantong mixture.
Objective To evaluate the effects of metformin on safety of newborn of woman with gestational diabetes mellitus (GDM) treated with metformin. Methods MEDLINE,CENTRAL,EMBASE,CNKI,WANG FANG DATA and CBM were retrieved. Randomized controlled trials (RCTs) that enrolled women with GDM treated with metformin were included. According to the inclusion and exclusion criteria,the quality of the extracted literature were evaluated by the Cochrane Risk of Bias assessment tool of Interventions5.1.0. The included studies were evaluated and analyzed by Meta-analysis with RevMan 5.3. Results Totally,11 RCTs enrolled 2056 pregnant women with GDM. The results of meta-analysis showed that Metformin significantly lowered the risk of neonatal hypoglycemia [RR=0.71,95%CI (0.57,0.88),P=0.002],macrosomia [RR=0.66,95%CI (0.47,0.91),LGA [RR=0.80,95%CI (0.64,0.99),P=0.04],and NICU admission [R=0.77,95%CI (0.64,0.92),P=0.005],and significantly reduce birth weight [MD=-85.46,95%CI (-148.22,-22.69),P=0.008],while did not increase the risk of SGA,NRDS,hyperbilirubinaemia,shoulder dystocia,perinatal fetal mortality,and congenital anomalies. Conclusion Metformin appear to be an effective and safe oral drug for the treatment of GDM.
Objective To investigate the role of clinical pharmacist in the treatment of heart transplantation patient accompanied acute kidney injury. Methods According to the characteristics of etiology in the earlier period of heart transplantation,clinical pharmacist analyzed the infection prophylaxis protocol,and took pharmaceutical care in drug interactions. Results Clinical pharmacist successfully assisted clinician to adjust the therapeutic regimen,reduced drug side effects caused by drug interactions. Conclusion Clinical pharmacist should pay attention to drug interactions and provide pharmaceutical professional service for heart transplantation patients,which ensures safe and effective drug therapy.
Objective To investigate the effect of clinical pharmacists’ intervention on ciclosprin blood concentrations and readmission rate of patients with aplastic anemia (AA). Methods Totally,190 patients with aplastic anemia orally treated with ciclosprin who discharged from Hematology Department between June 2014 to June 2015 were selected. They were randomly divided into intervention group and control group (each group with 95 cases). Pharmaceutical intervention was performed on intervention group. The rate of ciclosprin blood concentration reaching the standard and readmission rate were compared. Results The rate of ciclosprin blood concentration reaching the standard in intervention group (58.43%) was significantly higher than that in control group (30.59%),OR=1.91,95%CI(1.33,2.75),P<0.001,and the readmission rate (8.99%) was lower than that the control group (21.18%),OR=0.42,95%CI(0.20,0.92),P=0.024. The differences between the two groups were statistically significant. Conclusion Clinical pharmacists’ pharmaceutical intervention could raise the rate of ciclosprin blood concentration reaching the standard in patients with aplastic anemia,which could decrease the readmission and improve the therapeutic effect.
Objective To explore the role of clinical pharmacist in individual nutrition support for a patient with cervical cancer complicated with radioactive enteritis. Methods Clinical pharmacist provided medication advice and assisted with physicians to make the scheme of individual nutrition support. The pharmacist provided suggestions on the starting time of enteral nutrition,increased the dosage of amino acid by 17.3 g,additionally used alanyl glutamine injection 10 g and adjusted fat emulsion injection and amino acid in parenteral nutrition prescription for abnormal liver function. Meanwhile pharmacists gave patients reasonable suggestions about enteral at home. Results Clinical pharmacists participated in the treatment process and clinicians taked advice. After 10 days of enteral and parenteral nutrition,the patient got better and oral intake was resumed with the cooperation of clinicians and pharmacists. Conclusion Clinical nutrition pharmacists provide rational advice,which plays positive role in clinical medication.
