To investigate ① the role of international communication on scientific and technological innovation issues such as new drug research and development,pharmacological theory and technological innovation,education,personnel training; ② comments of the patterns to enhance communications with international experts and to use professional resources,thus to promote domestic scientific and technological innovation,to enhance personal training,and to strengthen the international influence of the society,eight pharmacologists from either China or abroad were interviewed. The successful experiences and existing problems were summarized from the interviews. The suggestions of future development were proposed to improve the level of international scientific communication of Chinese Pharmacological Society,and thus provide further reference for displaying functions of international scientific communication on the national system of scientific and technological innovation.
Objective To explore the mechanism of Xiaoaiping injection enhancing the inhibitory effect of paclitaxel on the proliferation of ovarian cancer cell A2780. Methods Ovarian cancer cell line A2780 were treated by Xiaoaiping injection (20,40,80,160 mg·mL-1) or paclitaxel(10,40,80,160 nmol·L-1) or Xiaoaiping injection combined with paclitaxel, respectively. The inhibition ratios of different drugs on the proliferation of A2780 cells were measured by MTT method. The changes in cell morphology after drug treatment were monitored by microscope. The mRNA expression of PXR, CAR and CYP2C8, CYP3A4, CYP3A5, transporter P-gp were detected by RT-PCR. The protein expression of PXR, CYP2C8 were measured by Western blotting. Results Xiaoaiping injection inhibits the proliferation of A2780 cells in a time- and concentration-dependent manner. Compared with paclitaxel treatment alone, Xiaoaiping injection combined with paclitaxel exhibit more significant inhibitory effect on A2780 cell proliferation RT-PCR and Western blotting results revealed that Xiaoaiping injection can significantly inhibit paclitaxel-induced over expression of PXR and CYP2C8. Conclusion Xiaoaiping injection can significantly enhance the inhibitory effect of paclitaxel on the proliferation of ovarian cancer A2780 cells. The mechanism may be associated with the inhibitory effect of Xiaoaiping on paclitaxel-induced overexpression of nuclear receptor PXR and its downstream drug metabolic enzyme CYP2C8.
Objective Detect the expression level of HMGB1,TLR4,TNF-α,IL-1β and IL-10 in spinal cord tissue of neuropathic pain model rat after tanshinone ⅡA treated to explore its effect on neuropathic pain and the mechanisms. Methods A total of 54 males Sprague-Dawley (SD) rats were randomly divided into three groups:sham-operated group , model control group, and Tan ⅡA group.Tan ⅡA was administered intraperitoneally to rats in Tan ⅡA group at a dose of 30 mg·kg-1 daily for 14 days after surgery.The pain threshold was measured 1 day before SNL (baseline) and 3, 7, and 14 days after surgery.The expressions of HMGB1, TLR4 mRNA and protein in lumbar spinal cord 4-6(L4-L6) were assessed by RT-PCR and Western blotting, respectively.The levels of TNF-α, IL-1β and IL-10 in the spinal cord were detected by ELISA. Results The paw withdrawl threshold (PWT) and paw withdrawl latency (PWL) were significantly decreased after SNL (P<0.05) .The expressions of TLR4, HMGB1 mRNA and protein were significantly increased (P<0.05),and the levels of TNF-α, IL-1β were significantly increased after SNL compared with those in the sham-operated group (P<0.05).After Tan ⅡA treatment, HMGB1 and TLR4 mRNA and protein levels were reduced significantly (P<0.05).TNF-α and IL-1β were downregulated, but IL-10 was upregulated in the spinal cords of SNL-induced rats (P<0.05),which was accompanied by improvement of pain behaviours in the Tan ⅡA group (P<0.05). Conclusion These results indicate Tanshinone ⅡA inhibited SNL-induced neuropathic pain via multiple effects, the possible mechanism is that HMGB1-TLR4 signal transduction pathway and its downstream cytokines are inhibited at the molecular level.It also suggesting that HMGB1-TLR4 signal transduction pathway may be related to neuropathic pain and is a target for the treatment of neuropathic pain.
Objective To explore the molecular mechanism of Gastrodia rhizoma in the prevention of headache, and to predict the potential target. Methods Using Integrated Pharmacological platform of traditional Chinese Medicine(TCM-IP) to predict the target based on Chemical Information of Gastrodia rhizoma and Construct the PPI (protein-protein interaction) network with target information for headache related diseases,enriching and analyzing the biological gene function and related pathways of Gastrodia rhizoma, diseases and key targets. constructing the interaction network with Chemical ingredients of Gastrodia rhizoma, key targets and drawing the “Gastrodia rhizoma -key Target-Pathway” Network. Results The active constituents of Gastrodia rhizoma Blume include ingphenols, polysaccharides and other ingredients, such as gastrodin, bis (4-hydroxyphenyl) ether beta-D-glucopyranoside, citricacid, xanthostrumarin/sitosterol 3-O-beta-D-pyran glucoside, hexadecanoic acid for the prevention of headache; 118 core target information was obtained for the prevention and treatment of headache in Gastrodia rhizoma, including 9 Putative Drug Target (potential drug target),such as GCK (Glucokinase),ATP1A1(Sodium/potassium transported alkaline phosphatase subunit α-1) and RPIA (5- phosphate isomerase A); 27 Known Disease Target , including HTR2B (serotonin receptor 2B), HTR1B (serotonin receptor 1B), HTR1D (serotonin receptor 1D), HTR1A (serotonin receptor 1A), PIK3CA (phosphatidylinositol 3-kinase) etc. Conclusion Based on Traditional Chinese medicine integrated pharmacology platform V1.0, this study analyzed the molecular mechanism of Gastrodia rhizoma in preventing and treating headache, and predicted the potential drug targets of Gastrodia rhizoma against headache, which will provide scientific basis for further experimental research of Gastrodia rhizoma.
Objective To characterize the liver uptake of the main toxic alkaloids of Fuzi including aconitine, mesaconitine and hypaconitine. Methods Celler uptake was studied by using HepG2 cells as an in vitro model, and the drug concentration was determined by liquid chromatography-mass spectrometry (LC-MS/MS). Results Aconitine, mesaconitine and hypaconitine were transported into HepG2 cells in a temperature-, concentration- and pH-dependent manner, which were significantly reduced by FCCP (a protonophore) and sodium azide (a metabolic inhibitor).The uptakes of Fuzi alkaloids were greatly inhibited by various cationic drugs such as quinidine, verapamil, pyrilamine, diphenhydramine and amantadine.However, the prototypical substrates or inhibitors of well-characterized organic cation transporter (OCTs), organic cation/carnitine transporters (OCTNs), multidrug and toxin extrusion transporters (MATEs) and plasma membrane monoamine transporter (PMAT) didn’t inhibit the cellular uptake of each Fuzi alkaloid. Conclusion Toxic alkaloids of Fuzi are mainly actively transported into liver cells, and the novel organic cation/proton antiporters may be the molecular basis for their entry into liver.
Objective To study the protective effect of grape seed oil (GSO) on methimazole (MMI) induced hypothyroidism mice, and to test its effect on the expression of serum inflammatory factors IL-1β,IL-2 and INF-γ. Methods A total of 24 female mice were randomly divided into three groups: normal control group, model control group and grape seed oil group (n=8). Animal model was constructed by gavage administration of MMI. Then the mice were sacrificed after 1 mg·kg-1·d-1GSO treatment for 4 weeks. The spleen, thymus and thyroid tissue were separated . The function of thyroid gland and the content of cytokines in mice were mesured. Results Body weight of normal control group was (23.54±1.06) g, model control group was (20.80±1.32) g and grape seed oil group was (20.28±0.82)g. GSO could reduce the spleen quality comparing with model control group(P<0.05). GSO treatment could increase the production of FT3 and FT4 (P<0.05) and significantly down-regulate the expression of IL-1β and IL-2 in serum. Conclusion GSO administration could provide protective effects on hypothyroidism through reducing inflammation and regulating the expression of IL-1β and IL-2.
Objective To investigate the effect of total saponins from Rhizoma Paris (RPTS) on radiosensitivity of hepatocellular carcinoma (HepG2) cells and its possible mechanism. Methods HepG2 cells were divided into 4 groups:blank control group, cultured with normal culture solution for 48 h; RPTS group, treated with RPTS (25 μg·mL-1) for 48 h; irradiation group, after culture with normal culture solution for 48 h, the cells were irradiated by 6 MV X ray; irradiation+RPTS group, after treatment with RPTS (25 μg·mL-1) for 48 h, the cells were irradiated by 6 MV X ray. CCK-8 was used to detect effect of total saponins on proliferation of HepG2. Influence of total saponins on cell cycle and apoptosis was detected by flow cytometry. The expression of MUC-1 protein was detected by Western blotting. Results The RPTS had inhibitory effects on HepG2 cells in a time and concentration dependent manner. The low cytotoxic concentration of RPTS regulated the cell cycle distribution of HepG2 cells.The low cytotoxic concentration of total saponins from Rhizoma Parisfurther induced apoptosis of hepatoma cells by X-ray(P<0.05).The low cytotoxic concentration of RPTS further inhibited the expression of MUC-1 protein (P<0.01). Conclusion RPTS have a certain radiosensitization effect on HepG2 cells, which may be related to the inhibition of cell proliferation, regulation of cell cycle, promotion of cell apoptosis and the reduction of the expression of MUC-1 protein.
Objective To investigate the anti-inflammatory,antitussive and in vitro bacteriostatic effects of the extract of Anchusa italica Retz. ,so as to scientific basis for clinical application. Methods The anti-inflammatory effect of different extracts was observed by inflammation model induced by carrageen glue toes swelling of rats; Antitussive effect was observed by ammonia-induced cough model; The in vitro bacteriostasis effects of different extracts on 5 strains (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans) was evaluate by the tube dilution method . Results The swelling of rat toes was significantly inhibited at different time after inflammation by 0.63 g·kg-1, 2.52 g·kg-1 of ethanol extracts from Anchusa italica Retz., 2.52 g·kg-1 of ethyl acetate and n-butanol parts (P<0.01), and The inhibitory effect of 2.52 g·kg-1 ethanol extracts on inflammation was similar to that of dexamethasone group, but there was no statistically significant difference between n-butanol parts and ethanol parts; In the antitussive experiment in mice, 0.90 g·kg-1, 3.04 g·kg-1 ethanol extracts, and 3.04 g·kg-1 of the n-butanol extracts had better effect than the jizhi syrup in prolonging the incubation period of cough in mice (P<0.05 or P<0.01), and the parts of the 3.04 g·kg-1dose group could significantly reduced the frequency of cough (P<0.05); All extracts had antibacterial activity against Bacillus subtilis, and the inhibition levels of ethyl acetate and water soluble parts were stronger. Conclusion The ethanol extracts and n-butyl alcohol parts has anti-inflammatory and antitussive pharmacological activities, and ethyl acetate parts has inhibitory activity against bacillus subtilis in vitro.
Objective To determine whether 7-methoxy-4’-hydroxyisoflavone promote maturation of osteoblasts. Methods Osteoblasts was treated by different concentrations (0, 10-4, 10-5, 10-6, 10-7, 10-8 mol·L-1) of 7-methoxy-4’-hydroxyisoflavone. The concentration of osteocalcin(OC) and alkaline phosphatase(ALP) were determined.The expression levels of protein sGC and PKG-1 in cells were detected by Western blotting. Results The group of 10-6 mol·L-1 significantly increased ALP activity and OC content in osteoblasts. The effect of L-NMA on ALP activity and OC content in osteoblasts was inhibited, and the expression of NOS, NO, cGMP, sGC and PKG was inhibited Conclusion 7-methoxy-4’-hydroxyisoflavone promoted osteoblasts differentiation through NO/cGMP/sGC signal pathway.
Objective To investigate the effect of flurbiprofen acetate on postoperative cognitive dyssfunction (POCD) and inflammatory factors after laparoscopy surgery in elderly patients. Methods Eighty patients undergoing laparoscopic cholecystectomy, were randomly divided into flurbiprofen acetate groups (group F, n=40) and control groups (group C, n=40). Before induction of general anesthesia, group F received intravenous injection of flurbiprofen 50 mg, and re-injected 50 mg 12 h after surgery. Group C received an equal volume of fat emulsion and re-injected an equal volume of fat emulsion 12 h after surgery. The visual analogue scale (VAS) was used for pain scores at 0, 6, 12, 24, 48, and 72 h after surgery.Neuropsychological testing was performed with mini-mental state examination (MMSE)on the preoperative day,1th and 3th postoperative day. The development of POCD was recorded within 3 days after surgery. Venous blood samples were taken before induction (to), immediately after surgery (t1), 12 hours after surgery (t2), and 24 hours after surgery (t3). Serum levels of interleukin-6(IL-6) and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA). Results Compared with group C, the resting VAS score decreased from 0 to 48 h after operation in group F (P<0.05). The MMSE scores of 48 hours before or after operation of the group C were (28.4±1.4) and (23.2±1.6), respectively, and that of group F were (28.6±1.4) and (26.0±1.8) , respectively; the postoperative score of the group F was significantly higher than that of the group C (P<0.05); The incidence of POCD in group C was 28% within 72 hours after operation, but 15% in group F, which was significantly lower than that in group C (P<0.05). Compared with group C, the serum concentrations of IL-6(29.3±6.2,32.8±8.0,30.7±7.1) ng·L-1were significantly decreased in group F(F<0.05).Compared with group C,the serum concentrations of TNF-α were (13.0±3.1,14.5±4.4) ng·L-1 at t1- t2 were significantly decreased in group F(P<0.05). Conclusion Flurbiprofen acetate can reduce the occurrence of cognitive dysfunction in elderly patients undergoing laparoscopic cholecystectomy,and its mechanism may be related to pain relief and inhibition of inflammatory response.
Objective The encephalic entropy index (M-Etronpy) was used as an indicator of anesthesia sedation depth to investigate the effect of two doses of remifentanil on the concentration of effect compartment and the depth of anesthesia during propofol-controlled infusion. Methods A total of 81 patients requiring general anesthesia were divided into A, B and C groups (n=27) according to different target concentrations of remifentanil.Group A received 0.9% sodium chloride injection for anesthesia induction and maintenance, group B received 2 ng·mL-1 remifentanil, group C was subjected to 4 ng·mL-1remifentanil for anesthesia induction and post-intubation 3 ng·mL-1 remifentanil for anesthesia maintenance.The level of sedation was assessed using OAA/S by the anaesthesiologist.The HR, MAP, SpO2, BIS values, SE, RE, and propofol effector chamber concentrations at different time points were recorded. Results Compared with group A, the concentrations of propofol in the group B and C were significantly decreased from t1 to t5 (P<0.05).At t6, the propofol effector concentration in group C was significantly lower than that in group A (P<0.05).The correlation coefficient between SE (r=0.84) and RE (r=0.85) and propofol effector chamber concentrations were similar.SE and RE decreased in all groups with decreasing OAA/S scores.Remifentanil infusion, in a concentration-dependent matter, resulted in higher values for SE and RE at LVC and LRP. Conclusion Induction and maintenance of anesthesia with remifentanil can reduce effector chamber concentration and propofol dosage during propofol target-controlled infusion induction, SE and RE to predict OAA/S, LVC and LRP are accurate.
Objective To compare the efficacy of rivaroxaban and warfarin plus aspirin in preventing thrombosis after iliac vein stent implantation, and to explore the application value of rivaroxaban in preventing thrombosis in iliac vein stent. Methods A retrospective analysis of 61 patients with iliac vein compression syndrome and stent placement in the first affiliated hospital of Zhengzhou university from January 2014 to June 2016 was conducted.The patients was divided into rivaroxaban group (treatment group,n=37) and warfarin + aspirin group (control group,n=24).Both groups were given the same promoting blood circulation and ant swelling drugs, and wearing the stretch socks after surgery.During hospitalization, low molecular heparin (LWMH) in 100 U·kg-1,q12h ,was given to prevent thrombosis.After discharge, the treatment group was adjusted to rivaroxaban with 20 mg,qd ,the control group was given warfarin plus aspirin after adjusting the standard of warfarin (INR2-3) and maintained this standard.The duration of anticoagulation was 6 months.The duration of anticoagulation was 6 months, and the stent patency was observed by ultrasound or venography in 1 month, 3 months, 6 months, 1 year and 2 years after surgery.Meanwhile, bleeding events were recorded. Results Sixty-one patients were followed up, and the incidence of thrombosis in stents in control group was 16.21% and that in treatment group was 12.50% (P>0.05).Total hemorrhage rate in control group and treatment group were 45.90%,33.33% (P>0.05), minor bleeding rate were 24.32%, 33.33%, respectively (P>0.05), severe bleeding were 21.60% and 0%, respectively(P<0.05).The differential has a statistical significance to the VCSS score of acute deep vein thrombosis (P<0.05). Conclusion Rivaroxaban did not increase the overall risk of bleeding, could reduce the risk of severe intracranial bleeding, did not increase the incidence of thrombus in the stent.The prognosis of patients with acute thrombosis was significant improved after use rivaroxaban.
Objective To analyze the effect of Huawei xiaoai fang combined with microwave ablation for treating liver cancer on aged patients. Methods A total of 60 aged patients with liver cancer were selected and divided into combine treatment group and microwave ablation group.The patients in the microwave ablation group were treated with ultrasound-guided percutaneous microwave ablation.The combine treatment group was treated with Huawei xiaoai fang on the basis of the microwave ablation group.The two groups were both to test the blood counts, liver function, PT, AFP,T cell immunity and Karnofsky performance status. Results The total effective rate of combine treatment group was 86.7%,much better than the microwave ablation group(73.3%)(P<0.05).After treatment, the levels of blood counts, liver function, PT,AFP and T cell immunity were significantly better than those of the microwave ablation group(P<0.05).The scores of Karnofsky performance status for the patients in the combine treatment group was significantly better than the microwave ablation group(P<0.05). Conclusion The efficacy of Huawei xiaoai fang combined with percutaneous microwave ablation for the treatment of elderly patients with liver cancer has the characteristics of small trauma, definite curative effect and high safety.It can improve the body immunity of patients, promote the activation of hematopoietic function of bone marrow, increase white blood cells and protect liver function.It has positive significance for improving the quality of life of patients.
Up to now, the results showed that some traditional Chinese medicine injections such as Salvia miltiorrhiza injection, Acanthopanax injection, Xueshuantong injection, Danhong injection, Shuxuetong injection and Safflower injection could enhance the anticoagulant effect of warfarin. The mechanisms include pharmacokinetic interactions such as influence on plasma protein binding of warfarin, inhibiting or inducing activities of warfarin-related cytochrome P450 metabolic enzymes, and pharmacodynamic influence. Some traditional Chinese medicine injections have a bleeding risk in combination with warfarin. Clinical studies have also shown that the use of synergistic anticoagulant effects in the treatment of certain thromboembolic diseases can have a beneficial effect on treatment, but there is still no evidence-based research and evaluation. Therefore, attention should be paid to the possible influence of traditional Chinese medicine injections on warfarin anticoagulation in clinic, and INR needs to be monitored closely for the patients, or adjust warfarin dosage appropriately, or avoid the combined use of the two kinds of drugs with mutual effects, so as to ensure safety of patient.
Objective To investigate the solubilization effects of mogroside V as a novel carrier on the poorly soluble drugs. Methods Using mogroside V as a carrier and paclitaxel as a model drug, a solid dispersion of paclitaxel-mogroside V was prepared by the solvent method.The content of paclitaxel was determined by high performance liquid chromatography (HPLC). The saturated solubility and in vitro dissolution of paclitaxel were investigated.In addition, differential thermal scanning (DSC) was used for phase identification and evaluation of drug crystal changes in solid dispersion . Results Paclitaxel-mogroside V solid dispersion showed pronounced improvement in both dissolution rate and drug solubility, as evidenced by a 375-fold increase in solubility compared with paclitaxel.The results of DSC indicated that paclitaxel was in amorphous form in solid dispersion. Conclusion Mogroside V can improve the solubility and in vitro dissolution of the poorly soluble drug.Being safe and non-toxic, mogroside V was found to have suitable characteristics to be used as a pharmaceutical excipient.
Objective To prepare galactosylated bovine serum albumin nanoparticles (Gal-BSA NPs) of curcumin by desolvation method, and to investigate its physicochemical properties and in vitro release characteristics. Methods Gal-BSA NPs were prepared by emulsion solvent evaporation method, and the preparation processes was optimized. Its size and Zeta potential were measured by Laser size and Zeta potential instrument. Entrapment efficiency and drug loading were measured by ultracentrifugation. The in vitro release characteristics were investigated by dialysis method. Results According to the preparation of the formulation and process optimization, the appearance of Gal-BSA NPs prove to be round or oval, particle size distribution was (267.1±78.3) nm, Zeta potential was -40 to -50 mV. Entrapment efficiency was 79.4%, and drug loading was 3.7%. Gal-BSA NPs release 20% at 8 h and >80% at 48 h. Conclusion Solvent evaporation method can successfully prepare Gal-BSA NPs, and shows good physical and chemical properties and release properties, improve the stability of the drug, and can prolong the drug release rate and improve the bioavailability.
Objective To establish a method to determine 36 metallic elements in Ipecacuanhae by microwave digestion-ICP-MS. Methods HNO3-H2O2 was used as the digestion system to treated samples by microwave digestion and determine 36 metallic elements content by ICP-MS with using Rh、Re as internal standard. Results The results showed that all the 36 metallic elements had good linearity in certain rang with the coefficient(r) higher than 0.999 5.The average recoveries were among 86.5% to 107.1%, with the relative standard deviation among 1.2% to 6.1%.The limits of quantification of 36 metallic elements were among 0.001 μg·L-1 to 0.968 μg·L-1. Conclusion The results demonstrated that the developed method is simple, rapid sensitive and reliable, and suitable for the determination of metallic elements.
Objective To establish a headspace gas chromatography method for two residual solvents determination in sodium morrhuate injection. Methods The residual solvents in sodium morrhuate injection were separated on DB-624 column(30 m×0.32 mm,0.18 μm)with an FID detector. Nitrogen was used as the carrier gas. The flow rate was 2.0 mL·min-1. The injector temperature and the detector temperature was set at 200 ℃ and 250 ℃, respectively. The containers of headspace injector were in equilibrium for 20 min at 80 ℃. Results The peaks of ethanol and diethyl ether could be effectively separated. Good linear relationship of ethanol and ether was obtained within the range of 20.08-1004 and 0.999-999.2 μg·mL-1(r≥0.999 7), respectively; The average recoveries (n=9) were 100.0% and 100.0%, respectively, and RSDs were 0.7% and 0.8%, respectively. Conclusion The method is simple, sensitive, accurate and reliable, and has good repeatability, and is suitable for analysis and detection of residual solvents in sodium morrhuate injection.
Objective To develop a simple and rapid method for simultaneous quantification of capecitabine and its active metabolite(5-fluorouracil )in human plasma by liquid chromatography—tandem mass spectrometry (LC-MS/MS) and apply it to clinical practice. Methods After a protein precipitation using acetonitrile, the supernatant taken from the centrifugal plasma samples was analyzed directly. With a mobile phase comprising acetonitrile/0.1% aqueous formicacid and 25 mmol·L-1ammonium acetate (95:5) and an electrospray ion (ESI) source, samples contained capecitabine and 5-fluorouracil were quantitative analyzed. The MS/MS detection was performed using multiple reaction monitoring (MRM), in positive mode. The mass transitions of the protonated precursor/product ion pairs were used to record the selected ion mass chromatograms of capecitabine and 5-fluorouracil were m/z 358.1→154.1 and m/z 129→42.1, respectively. 20 patients were receiving chemotherapy with capecitabine were then the steady state trough concentration of capecitabine and 5-fluorouracil in the patients’ plasma were monitored. The clinical application of the detection method was evaluated through the correlation of single point concentration of capecitabine and 5-fluorouracil. Results The linear calibration curve was obtained in the concentration range from 39.062 5-10 000 ng·mL-1(R2=0.999)for both capecitabine and 5-fluorouracil with the limit of quantification was 39.062 5 ng·mL-1. The extraction recoveries of capecitabine at three concentration levels(78.125,625 and 5000 ng·mL-1) and that of the 5-fluorouracil were (78.22±3.15)%,(75.37±2.99)%,(81.05±2.78)% and (70.45±2.19)%,(72.56±1.68)%,(72.02±1.72)%,respectively. The intra-and inter-run precisions,expressed as the relative standard deviation(RSD)were less than 5% for both of capecitabine and 5-fluorouracil. The detection result acquired from the plasma samples indicated that there was differences for the plasma concentration of capecitabine and 5-fluorouracil between individuals and that there was a poor correlation of plasma concentration between the capecitabine and its active metabolite. Conclusion The method is sensitive,specific and validate rapid for simultaneous quantification of capecitabine and 5-fluorouracil in human plasma by liquid chromatography—tandem mass spectrometry and will be successfully applied for clinical practice to improve the effectiveness and security of chemotherapy regimens with capecitabine.
Objective To promote its rational application and provide reference for drug administration by comparing the application of proton pump inhibitors before and after intervention. Methods The unreasonable use of proton pump inhibitors in the department of cardiology was intervened through the development of evaluation criteria for rational drug use, training on rational drug use, comments and communication.and administrative intervention.The total medication use of PPI in cardiology department before and after intervention was compared, including sales amount, utilization rate and drug proportion. 150 cases of hospitalized patients using PPI injection before and after the intervention were randomly selected for rationality analysis. Results Before the intervention, the number of patients with PPI was 2171, it was 1780 after intervention, the decrease rate was 18.01%; The sales amount was changed from 0.9139 million to 0.5587 million, the decline rate was 38.87%.After intervention, the utilization rate of PPI decreased significantly, by 23.57% (P=0.000), and the proportion of PPI drugs was significantly lower than before, too, the decline rate was as high as 51.12%(P=0.000).The proportion of patients who did not have indication of PPI injection was significantly reduced from 17.33% to 5.33%(P=0.001).Prevention using PPI medication indications grasp tighter (P<0.05), and the situation of overdose medication was significantly reduced (P=0.000), the situation of improper drug selection and drug interaction disappeared completely, and the rationality of medication was significantly improved. Conclusion The pharmaceutical intervention of clinical pharmacists can effectively promote the rational use of PPI in cardiology department, and the intervention mode can be used for the special management of other drugs.
Objective To retrospectively investigate the clinical features of patients with pulmonary involvement in propylthiouracil (PTU)-associated antineutrophil cytoplasmic autoantibody vasculitis (AAV).The aim of our study was to summarize these data of the reasonable medication and the follow-up treatment for clinician physicians. Methods Clinical features and outcomes of 5 patients developed AAV with pulmonary involvement after treatment with PTU were collected and analyzed.ANCA was detected by both immunofluorescence (IFT) and enzyme linked immunosorbent assay (ELISA). Results Five patients, male 2,female 3, average age(52.7±5.4) years, PTU for 3-10 years, received when PTU-AAV was diagnosed.All patients had positive serum p-ANCA, 4 of them were MPO-ANCA, 1 of them was MPO and PR3-ANCA double positive.All patients present with fever and cough.Two patients of them suffered from dyspnea and developed respiratory failure.Initial diagnosis of all patients was misdiagnosed as pulmonary infection.All patients got renal involvement presented with hematuria and/or proteinuria.All patients withdrew PTU and received steroid therapy,2 of them were treated with cyclophosphamidesimultaneously.All patients got clinical remission with no recurrence during the follow-up period. Conclusion It is important to comprehend PTU-induced AAV and distinguish it from primary vasculitis for clinicians.PTU should be discontinued immediately after diagnosis.Corticosteroids and immunosuppressive agents may be promptly used in patients with vital organ involvements.
Objective To construct a comprehensive evaluation system for ADR report quality for a more comprehensive analysis and evaluation of the quality status of ADR report forms. Provide reference for relevant management departments to strengthen post-marketing drug management and improve the quality of ADR reports. Methods Combine the questionnaire survey method with the analytic hierarchy process to construct the ADR report quality evaluation system. Results This study constructed a set of quality evaluation index system for adverse drug reaction reports, including the specific scoring criteria and score weights of 65 project indicators in the ADR report, which basically included the main indicators in current “Adverse Drug Reaction/Event Report Form” in China. Conclusion Using the established ADR report scoring system, the report's indicators are evaluated and scored, and the overall system quality status can be reported, so that the report quality can be intuitively and comprehensively understood, and the specific problems that the reporter has in the report process are intuitive. In the case of which project indicators are found to be problematic, and then targeted to intervene in the quality of the report, thereby improving the quality of the report from the root cause.
Objective The literatures related to pharmacovigilance in China were analyzed quantitatively to provide references for the research on the current situation of pharmacovigilance in China. Methods “Pharmacovigilance” was choosen as the term in literature searching in CNKI, VIP and Wanfang database.The results were analyzed with regard to the number of articles, research level, keywords, organization, authors, journal of publication and total citations.The citation analysis was done automatically by the database. Results A total of 588 literatures concerning pharmacovigilance were recorded in CNKI.The first literature was published in 1997, after which the total number of publications and citations keep increasing until 2009.There was an Explosive increase of publications in 2009.Most literatures were published by National food and drug administration drug evaluation center(7.99%).Zhang Bing had published 24 papers,which was the most of all. Conclusion At present, China is increasingly concerned about the research on pharmacovigilance, but the original research work based on China's national conditions needs to be more in-depth and requires the attention, investment and participation of experts from the government.