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    药物研究
  • 药物研究
    Effect of Injection Site and Doseage of Basic Fibroblast Growth Factor on Rats Peripheral Nerve Regeneration
    LIU Huanxing;JI Rixu;MA Guangxi;SHEN Xinsheng;CAI Zhongqing;WANG Ping
    2014, 33(1): 5-7. https://doi.org/10.3870/yydb.2014.01.002
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    ABSTRACT Objective To explore the effect of administration method and dosage of basic fibroblast growth factor （bFGF） on rats peripheral nerve regeneration .MethodsA total of 45 rats were injured by sciatic nerve cut and sutured.Nerve microscopic suture was applied to repair nerve before rats were divided into five groups.After operation ，groups A，B， C were injected with 800， 400， and 200 U of bFGF solution in the muscle，respectively.For group D，200 U was injected in multiple sites.For group E，equivalent saline was injected.Motor nerve conduction velocity，muscle evoked action potential and fibrillation potential amplitude were evaluated by electromyography 8 weeks after the operation.ResultsCompared with the control group， the motor nerve conduction velocity and muscle evoked action potential were significantly higher in the groups of bFGF and the fibrillation potential amplitude was lower in bFGF groups （P＜0.05）.The multiplesite bFGF injection showed remarkably higher motor nerve conduction velocity， muscle evoked action potential and lower fibrillation potential amplitude （P＜0.05）.No significant difference was showen between groups of different doses of bFGF in motor nerve conduction velocity， muscle evoked action potential and fibrillation potential amplitude （P＞0.05）.ConclusionThe bFGF injected at targeted sites in muscle can promote nerve regeneration in rats.The method of administration and the dose may influence the nerve regeneration.
  • 药物研究
    Effects of Bradykinin on Migration of Pulmonary Artery Smooth Muscle Cells Induced by TGFβ1
    FENG Wenjing;ZHAO Gang;XU Xizhen;ZHAO Junjie;DONG Ruolan;TU Ling;YAO Jihua
    2014, 33(1): 8-12. https://doi.org/10.3870/yydb.2014.01.003
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    ABSTRACT Objective To study the effects of bradykinin （BK） on pulmonary artery smooth muscle cells （PASMCs） migration induced by transforming growth factorβ1 （TGFβ1） and their possible mechanisms.MethodsThe transmembrane migration of BK on primary PASMCs with TGFβ1 treatment was examined by transwell assay， and the lateral migration of BK on primary PASMCs with TGFβ1 was determined by healing of surface scratch assay.ResultsTGFβ1 significantly increased the number of PASMCs migrating across the membrane （P＜0.05）. BK significantly reduced TGFβ1induced PASMCs transmembrane migration （P＜0.05）， which was not prevented by the B2 receptor （B2R） inhibitor HOE140 （P＞0.05）.Furthermore， BK significantly reduced TGFβ1induced PASMCs lateral migration （P＜0.05）， while the effect was not blocked by the B2R inhibitor HOE140 （P＞0.05）.ConclusionBK can significantly inhibit TGFβ1induced PASMCs transmembrane and lateral migration.However the inhibitory effects of migration may not be mediated by bradykinin B2 receptor.
  • 药物研究
    Effect of Mizoribine on p27 of Mesangial Cells in Rats with Acute Thy1 Nephritis
    QIN Fufang;SUN Qian;SHAO Fengmin
    2014, 33(1): 13-16. https://doi.org/10.3870/yydb.2014.01.004
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    ABSTRACT Objective To study the effect of mizoribine（MZ） on p27 of mesangial cells in rats with acute Thy1 nephritis.MethodsNinety male rats were randomly divided into five groups， which were normal， nephritic， mizoribine treated， losartan treated， both mizoribine and losartan treated groups.Each group had 20 rats except for normal group， which had ten rats.The nephritis model was induced and renal tissue was removed 1， 3， 5， 7 days after model was established.Mesangial cells proliferation was determined by PAS staining.The expression of p27mRNA was detected by RTPCR， and the protein expression was tested by western blotting.ResultsCompared with the control， the mesangial cells proliferation in the nephritis model group was remarkably increased，but the expression of p27 protein was decreased significantly（P＜0.05） .Compared with the nephritis models， treatment with MZ， losartan or MZ plus losartan showed an obvious inhibition of the mesangial cells proliferation（P＜0.05）， but significantly upregulated expression of p27 protein（P＜0.05），especially with the treatment of mizoribine， and losartan plus mizoribine.（P＜0.05）.ConclusionBoth mizoribine and losartan can inhibit mesangial proliferation in Thy1 nephritis，and the effect of mizoribine is more remarkable by upregulation of the expression of p27 protein.
  • 药物研究
    Components Study in Rat Blood After Oral Administration of Extracts from Purplish Red Ginseng
    QIU Nannan;LIU Jinping;LU Dan;LI Pingya
    2014, 33(1): 16-19. https://doi.org/10.3870/yydb.2014.01.005
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    ABSTRACTObjectiveTo analyze components absorbed in the blood of extracts from purplish red ginseng and illustrate its effective substance.MethodsThe serum sample was collected after oral administration of the extract of purplish red ginseng in rats， and the shift components were identified by UPLCESIMS/MS.ResultsFourteen transitional components in rat’s serum were detected， six were constituents absorbed in blood in prototype of extracts and the others might be irritability compositions or metabolites of purplish red ginseng in blood.ConclusionThe fourteen constituents absorbed into blood may be the bioactive components that act directly on the body， laying a foundation for studing active components and therapeutic mechanism of purplish red ginseng.
  • 药物研究
    Longterm Toxicity Tests of Biejiajian Pills in Rats
    XIONG Jing;LI Xia;ZENG Fanbo;CHEN Peng;ZHAI Li
    2014, 33(1): 20-23. https://doi.org/10.3870/yydb.2014.01.006
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    ABSTRACT Objective To study the longterm toxicity of biejiajian pills in rats， determine the safe dose，and provide reference for human use.MethodsSixty SD rats were divided into low， high dose groups， and control group，respectively （n=20 in each group）， biejiajian pills were orally administered to rats at the doses of 5 and 10 g•kg－1 daily for 6 months， while the control group was given with 2 mL of distilled water only.The general state， body mass and food intake were measured.By the end of the test and 4 weeks after drug withdrawal， blood routine， hematologic and biochemical examination，organ coefficient， and histopathology were tested.ResultsCompared with the control group， no significant differences in all checked items were found in the treatment groups.ConclusionBiejiajian pills have a low longterm toxicity on the organs， blood routine， hematologic and biochemical indicators.The organ specific toxicity of biejiajian pills is not found.Biejiajian pill at tested doses is safe for oral use in rats.
  • 药物研究
    Therapeutic Effect of Astragalus Injection Combined with Acupuncture Treatment for Rats with Chronic Glomerulonephritis
    ZENG Ming;CHENG Ying
    2014, 33(1): 24-27. https://doi.org/10.3870/yydb.2014.01.007
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    ABSTRACT Objective To investigate the therapeutic effect and mechanism of intraperitoneal （ip） injection of astragalus combined with acupuncture at zusanli and shenshu points on rats with chronic glomerulonephritis.MethodsFifty SD rats were randomly divided into blank control， combination therapy， astragalus injection alone， acupuncture alone and model groups（n＝10）. Intravenous injection of cationic bovine serum albumin was performed in all groups except the blank control， resulting in a rat model of chronic glomerulonephritis.The combination therapy group received daily ip injection of 1.5 mL astragalus （200 g）， with needling at zusanli and shenshu points on one side， each for 10 min.The astragalus group was injected ip with 1.5 mL astragalus （200 g） daily.The acupuncture group was treated with needling at zusanli and shenshu points at one-side， each for 10 min.The model group received normal feeding.The blank controls were injected ip with 1.5 mL sterile water for.Serum creatinine， blood urea nitrogen， and 24 h urine protein were determined.ResultsAll of the blood indexes were lower in the three treatment groups than those in the model one （P＜0.05）.ConclusionAll three therapies can slow the progress of chronic glomerulonephritis and protect kidney function.The combination of acupuncture with astragalus injection has the best effect， and astragalus injection alone shows better effect than acupuncture alone.
  • 药物研究
    Determination of Pterostilbene in Rat Plasma by High Performance Liquid ChromatographyTandem Mass Spectrometry
    GUO Runzheng;WU Chunjiang;ZHANG Yan;HUANG Qihui;LI Haixia;WANG Xu;CAO Zhonghou;MAO Yuhua;SHEN Jie
    2014, 33(1): 27-30. https://doi.org/10.3870/yydb.2014.01.008
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    ABSTRACT Objective To establish a liquid chromatographytandem mass spectrometry （LCMS/MS） method for determination of pterostilbene in rat plasma.MethodsThe plasma samples were extracted with ethyl ether and then separated on Thermo Hypersil Gold column （2.1 mm×50 mm， 5.0 μm），and guarded by a Thermo C18 column （4 mm×3.0 mm，10.0 μm）.The mobile phase consisted of acetonitrilewater （containing 1 mmol•L－1 ammonium formate） as gradient elution， and the flow rate was 0.3 mL•min－1.The injection volume was 10 μL and resveratrol was used as the internal standard （IS）.Electrospray ionization （ESI） source was applied and operated in a negative mode using multiple reaction monitoring （MRM） .Pterostilbene and transresveratrol were detected on MRM by transition from the precursor to production （m/z 255.1→240.2 and 227.1→143.0）.The total run time was 5 min.ResultsThe retention time of pterostilbene and resveratrol was 2.07 and 1.90 min， respectively.No endogenous interfering peaks were observed.The linearity was obtained within the range of 1－100 ng•mL－1.The precision （RSD） of inter and intraday was less than 15%and the accuracy was 103.1%， 98.12%and 93.23% at low， mid， and high concentrations （3，50，80 ng•mL－1）， respectively.The extraction recovery of pterostilbene was 78.6%， 80.3% and 83.2%， respectively， and no matrix effect was observed.ConclusionThe method is simple， sensitive， accurate and reliable， which is suitable for pharmacokinetics study of pterostilbene.
  • 药物研究
    Determination of Cyclosporin in Whole Blood by SolidPhase Extraction and High Performance Liquid Chromatography
    SONG Xinwen;WANG Yang;LIU Xin;PENG Jing;XU Qiong
    2014, 33(1): 31-33.
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    ABSTRACT Objective To establish a simple highperformance liquid chromatography （HPLC） method to determinate cyclosporin in whole blood.MethodsThe HPLC was carried out by using Venusil ASB C18 column， acetonitrile/water（70：30）as a mobile phase， setting column temperature at 70 ℃， detection wavelength at 210 nm.Whole blood samples were centrifugated after treated with 10%urea for hemolysis.Two hundred microliters of the supernatant was directly injected into the PRS  SPE column， using equal volume of acetonitrile/tetrahydrofuran（ 80：20） as the eluent.ResultsThe method is linear at 30 to 3 500 ng•mL－1 curve range.The recoveries at low， medium and high concentrations （78.2， 300.0， 1 213.0 ng•mL－1） were 101.66%， 104.06%and 100.46%， respectively.The intra and interday RSD were less than 5%.ConclusionThe method is accurate， sensitive， and the sample is easy to handle， fully meeting the requirement of pediatric clinical practice for determination of cyclosporin in whole blood.
    • 肿瘤科用药专栏
  • 肿瘤科用药专栏
    Inhibition Effects of Arsenic Trioxide on SHG44 Human Glioma Cells
    HUANG Hui;JIANG Hongling;LIU Fenju;BAI Xue;HE Zhuokai;CAI Rui;ZHANG Rongjun;ZHAO Bo;LIU Meilian
    2014, 33(1): 34-38.
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    ABSTRACT Objective To investigate the antiproliferation effects of arsenic trioxide（As2O3）on human glioma cells44（SHG44） in vitro and its mechanism.MethodsThe inhibitory ratio of the cells by As2O3 was measured by MTT assay.The intracellular ROS were determined by a probe （ DCFHDA） under a laser scanning confocal microscopy.ResultsAs2O3 significantly inhibited SHG44 cells proliferation.The proliferation inhibition rate was dosedependent and timedependent.The proliferation inhibition by 12 μmol•L－1 As2O3 on SHG44 cells was as high as 63.8%.After treatment with As2O3， the intracellular ROS was increased markedly in a concentrationdependent manner.ConclusionAs2O3 can inhibit the proliferation of SHG44 cells in both dosedependent and timedependent manners.The inhibition was associated with elevation of intracellular ROS .
  • 肿瘤科用药专栏
    Acute Myeloid Leukemia Secondary to Multiple Myeloma Treated with Decitabine：One Case Report
    WANG Qian;CHEN Dongmei;ZHANG Hong;FAN Yinyin;LI Jun;LI Bingzong
    2014, 33(1): 38-42. https://doi.org/10.3870/yydb.2014.01.011
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    ABSTRACT Objective To explore the clinical characteristics of acute myeloid leukemia secondary to multiple myeloma and therapeutic effect of decitabine.MethodsA 62yearold female who developed acute myeloid leukemia secondary to multiple myeloma remitted 3 years after the VAD chemotherapy followed by phenyl alanine nitrogen mustard maintenance therapy.No significant improvement was observed in with DA regimen.The patient was then treated with decitabine intermittently for two courses.ResultsThe bone myeloid progenitor cells of the patient were decreased significantly， and the blood routine examination recovered.The patient only received supportive treatment for economic reasons thereafter.ConclusionThe pathogenesis mechanisms of acute myeloid leukemia secondary to multiple myeloma is far from elucidated.Treatmentrelated factors and disease involvement may be the main contributions.Decitabine maybe the first choice for disease with poor prognosis.
  • 肿瘤科用药专栏
    Effect of Gefitinib and Everolimus on Nasopharyngeal Carcinoma Cells Resistant to Epidermal Growth Factor ReceptorTyrosine Kinases Inhibitor （EGFRTKI）
    LIU Yuan;LIU Dongbo;LONG Guoxian;HU Guoqing
    2014, 33(1): 43-48. https://doi.org/10.3870/yydb.2014.01.012
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    ABSTRACT Objective To investigate the synergistic effect and molecular mechanism of the epidermal growth factor receptor （EGFR） inhibitor gefitinib and the mammalian target of rapamycin （mTOR） inhibitor everolimus on nasopharyngeal carcinoma resistant to gefitinib in vitro.MethodsHuman nasopharyngeal carcinoma cell line HONE1 were incubated with gefitinib and everolimus alone and in combination， the effect of cell growth inhibition was measured by MTT assay. Flow cytometry was applied to assess the cell cycle and apoptosis. Western blot was used to detect the expression of phosphorylated levels of protein serine threonine kinases（pAKT） and ribosomal S6 kinases（pS6K） in cells. ResultsThe effects of cell growth inhibition by gefitinib or everolimus separately were dosedependent in the HONE1 cell line. The IC50 value for gefitinib was 17.92 μmol•L－1， and that for everolimus was 2.46 nmol•L－1. However， the combination of two agents did not show a obvious advantage over the drug used alone（P＞0.05）. The inhibition of pAKT expression was not remarkable in the HONE1 cell line after gefitinib treatment. Everolimus was revealed to downregulate the expression of poS6K and upregulate the level of pAKT in the HONE1 cell line. Moreover， no stronger inhibitory effect on pS6K and pAKT expression by combined agents was found. ConclusionCombined treatment of gefitinib and everolimus doesn’t show a synergistic effect against nasopharyngeal carcinoma cell line HONE1 resistant to gefitinib. Further research is in need to investigate the relationship between the EGFR/AKT signaling pathway and the mTOR signaling pathway in nasopharyngeal carcinoma cells.
  • 肿瘤科用药专栏
    Metaanalysis and Therapeutic Effect of Xiaoaiping Injection Combined with Chemotherapy for NSCLC
    TAO Xiaoxin;YU Hao;BAI Jianling
    2014, 33(1): 48-53. https://doi.org/10.3870/yydb.2014.01.013
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    ABSTRACTObjectiveTo evaluate the clinical efficacy and safety of xiaoaiping injection combined with chemotherapy on patients with nonsmall cell lung cancer（NSCLC）.MethodsThe inclusion and exclusion criteria and retrieval strategies of original documents， computer retrieval of CNKI （19792012）， WEP database （1989－2012） and the journal of digital periodicals （1998－2011 ） and Pubmed database with computer were developed by using Meta analysis methods， and the quality of literatures was assessed according to Cochrane review criteria.ResultsEleven researches were included.It was shown that the efficacy of xiaoaiping injection combined with chemotherapy was 50.41%（95%CI：45.12%－55.70%）， 1.28 times（95%CI：1.09－1.52，P＜0.01） of chemotherapy alone （39.44%， 95%CI：34.33%－44.62%）.Xiaoaiping injection combined with chemotherapy improved quality of life 1.90 times as much as chemotherapy alone （95%CI：1.55－2.33，P＜0.01）， and lowered the incidence of leukocyte reduction by 25%in comparison to chemotherapy alone（RR=0.75， 95%CI：0.59－0.96，P＜0.05）.No significant difference in occurrence of adverse reactions was observed between the two treatments.ConclusionThe combination of xiaoaiping injection with chemotherapy can improve the efficacy and quality of life， reduce the incidence of leukocyte reduction compared with using chemotherapy alone for patients with NSCLC.
  • 肿瘤科用药专栏
    Therapeutic Effect of Nedaplatin Combined with Radiotherapy for Elderly Patients with Esophageal Carcinoma
    LONG Guoxian;WU Jie;SHI Lei;LIU Shunfang;HU Guangyuan
    2014, 33(1): 54-56.
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    ABSTRACT Objective To evaluate the efficacy and safety of nedaplatin combined with radiotherapy on elderly patients with unresectable esophageal carcinoma.MethodsA total of 36 patients with unresectable esophageal carcinoma were randomly divided into study group and control group， each group has 18 patients.The study group received concurrent chemotherapy with nedaplatin 35 mg•（m2）－1 weekly combined with radiotherapy.The control group received radiotherapy only.Response rate and adverse event of both groups was evaluated.ResultsThe response rate in study group was 83.3%， which was superior to that in the control group （61.1%，P＜0.05）.The incidence of leukocytopenia， nausea and vomiting， and radiation esophagitis in the study group was higher than that in the control group （P＜0.05）.Adverse events of grade 3 or 4 were rare.ConclusionNedaplatin combined with radiotherapy could improve shortterm effects and is well tolerated for elderly patients with unresectable esophageal carcinoma.
  • 肿瘤科用药专栏
    Pharmaceutical Care of Hematopoietic Stem Cell Transplantation for the Treatment of Malignant Lymphoma
    ZHANG Xiao;SHI Guobing;ZHAO Qingchun;FAN Rong
    2014, 33(1): 56-59.
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    ABSTRACT Objective One case of lymphoma treated with the autologous hematopoietic stem cell transplantation was analyzed. MethodsThe clinical pharmacists participated in the whole process of the patient’s transplantation and proposed the pharmaceutical suggestion based on the patient’s clinical symptoms and medications.ResultsIt made the patient survive the initial transplantation and significantly ameliorate the complications of posttransplantation. ConclusionThe clinical pharmacists help doctors to optimize therapeutic regimens and minimize the adverse reaction. Therefore， the patient’s quality of life is improved.
    • 药物制剂与药品质量控制
  • 药物制剂与药品质量控制
    Study on HPLC Fingerprint of Rosa Roxbughii Tratt.
    ZHOU Xin;CHEN Huaguo;ZHANG Ming;YANG Shilin
    2014, 33(1): 82-85.
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    ABSTRACTObjectiveTo establish an HPLC method for determining fingerprint of Rosa roxbughii. MethodsThe HPLC method was used with Hanbon Phecda C18 column（250 mm × 4.6 mm，5 μm）. The mobile phase consists of acetonitrile（A） and 0.05%phosphoric acid （B） with a gradient elution. The column temperature was set at 25 ℃， and the detective wavelength was 255 nm. The HPLC fingerprint for 14 batches of Rosa roxbughii was studied. The similarity analysis and cluster analysis were taken to classify the herbs and establish a common pattern of fingerprints.ResultsA HPLC method for determination of ingredients in Rosa roxburghii tratt and the fingerprint were established. A unique fingerprint was presented for herbs from different areas. ConclusionThe establishment of HPLC feature fingerprint of Rosa roxburghii Tratt provides scientific basis for herbs quality control.
  • 药物制剂与药品质量控制
    Study on HPLC-PDA Fingerprints of Xianggui Huazhuo Capsule
    ZHANG Chenchen;YANG Jizhang;WU Dan;LIU Hongmiao
    2014, 33(1): 86-89.
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    ABSTRACT Objective To establish a HPLCPDA fingerprint analysis for the xianggui huazhuo capsule， and improve the quality control standard for it. MethodsHPLC separation was carried out on DiamonsilTM C18 column （250 mm×4.6 mm，5 μm）. The mobile phase was composed of methanol， acetonitrile and 0.1%glacial acetic acid with gradient elution. The flow rate was maintained at 1.0 mL•min－1 and the column temperature was set at 30 ℃. The detective wavelength was 280 nm. ResultsUnder the same chromatographic conditions， the similarity of eight batches of xianggui huazhuo capsule was above 0.9， and the chemical composition was consistent among different batches. ConclusionThe operation is simple and accurate， and therefore can be used for the identification and quality control of xianggui huazhuo capsule.
  • 药物制剂与药品质量控制
    Optimization of Reflux Extraction Process for Shiyifang Vinum
    MO Xiaolin;WU Xiaoyan;GONG Minyang;CHEN Xiaoming;TAN Dongdong
    2014, 33(1): 90-93. https://doi.org/10.3870/yydb.2014.01.026
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    ABSTRACT Objective To optimize the reflux extraction process of shiyifang vinum.MethodsThe preparation technology of shiyifang vinum was optimized by adopting orthogonal test on concentration， wine quantity， reflux time as investigation factors and taking ginsenoside Rg1， content of chrysophanol，kaempferol and total extraction as references.ResultsThe optimum process of reflux extraction for shiyifang vinum was extracting for 3 hours with six times of wine（50%） .ConclusionThe optimized process is reasonable and feasible，which is the good evidence for preparation of shiyifang vinum.
  • 药物制剂与药品质量控制
    Optimizing the Toxicity Reduction of Sand Fried Fructus Xanthii
    WAND Ying;FU Bin;ZHANG Hongqi;XIAO Anju
    2014, 33(1): 93-96. https://doi.org/10.3870/yydb.2014.01.027
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    ABSTRACTObjectiveTo explore the optimum process of reducing toxicity of the sand fried Fructus xanthii. MethodsThe influence of temperature， time and ratio of medicine to sand on contents of the toxic constituents of Fructus xanthii was studied by taking the single factor test and orthogonal test. ResultsThe optimal process was setting the temperature at 140 ℃， mixing medicine to sand at 1：15（g•g－1）ratio， and processing for 12 min. ConclusionThe extraction process is economic and repeatable， and therefore can be used for reducing toxicity of sand fried Fructus xanthii.
  • 药物制剂与药品质量控制
    Mechanism of Ionic Liquids as a Mobile Phase Additives on the Separation of Phellodendrine and Magnoflorine by HPLC
    YANG Yong;BIAN Min;LI Wei
    2014, 33(1): 96-99. https://doi.org/10.3870/yydb.2014.01.028
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    ABSTRACT Objective The chromatographic separation of phellodendrine and magnoflorine was established， and the mechanism of ionic liquid in the process was studied by investigating influencing factors.MethodsPhellodendrine and magnoflorine were isolated by HPLC with 1butyl3 methyl imidazole four fluorine boric acid as a mobile phase additive， and mobile phase pH， ionic strength， buffer solution， ionic liquids and column temperature were all studied by investigating the effect of ionic liquid in the process. ResultsIt was shown that the analytes were separated at baseline with a mobile phase containing ionic liquid under 20 mmol•L－1 isocratic elution. The retention value， separation and number of theoretical plates varied with the change of mobile phase. ConclusionIt is clarified that ionic liquid mainly acts as neutral ion pair in the separation of phellodendrine and magnoflorine.
  • 药物制剂与药品质量控制
    Determination of Effective Constituents in Fructus Arctii by Wavelength Switching Method of HPLC
    SUN Yantao;ZHAO Lanying;LI Tingting;ZHAO Lei;JIANG Dayu;
    2014, 33(1): 100-102. https://doi.org/10.3870/yydb.2014.01.029
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    ABSTRACT Objective To establish an HPLC method for simultaneous determination of three active components in Fructus arctii.MethodsThe HPLC analysis was carried out on TCC18（4.6 mm×250 mm，5 μm） column at the temperature of 25 ℃. The mobile phase composed of methanol and 0.4%glacial acetic acid with gradient elution at a flow rate of 1.0 mL•min－1. The detection wavelength was 280 nm shifted to 326 nm. The sample loading volume was 10 μL. ResultsThe linear range for chlorogenic acid， arctiin， and arctigenin was 0.027 3－5.460 0， 0.150 0－18.000 0， and 0.030 3－6.060 0 μg， respectively. The linear equation was Y=1 106.476X－11.452， Y=1 026.014X+20.391， and Y=287.661X+4.045， respectively. The average recovery of chlorogenic acid， arctiin， and arctigenin was 99.55%，99.42%and 99.40%， respectively. ConclusionThe method is quick， accurate， and reproducible. It can be used for content determination and quality control for Fructus arctii.
    • 用药指南
  • 用药指南
    Compatibility Test of βlactams with Pethidin and Propacetamol
    YANG Shunjuan;LIN Shuyu;PAN Danting;GAN Huizhen
    2014, 33(1): 108-110. https://doi.org/10.3870/yydb.2014.01.032
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    ABSTRACT Objective To explore the reason for white crystalline development in dualchannel infusion tube when combing βlactams with pethidin and propacetamol in the microinfusion pump. MethodsThe reason of incompatibility was explored by repeated simulating tests for the same drugs’ combination. ResultsThe white precipitation occurred in the dualchannel infusion tube when pethidine encountered βlactams. Propacetamol for injection was unstable. Decomposition products of propacetamol lowered pH of the infusion to 3.34 after drug combining for three hours. After the acid nonresistant drugs such as βlactams were added to the infusion liquid， with pH dropped from 5.37 to 1.87 over 24 h， and white crystals gradually developed. ConclusionPropacetamol in the infusion pump should not be used as intravenous drip for a long time. If used， propacetamol should be infused alone. The infusion pump liquids containing Pethidine should not be infused with βlactams simultaneously in the multichannel infusion tube.
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