中国科技论文统计源期刊　中文核心期刊  
美国《化学文摘》《国际药学文摘》
《乌利希期刊指南》
WHO《西太平洋地区医学索引》来源期刊  
日本科学技术振兴机构数据库（JST）
第七届湖北十大名刊提名奖  

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  • 01 December 2015 Volume 34 Issue 12
      
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  • Inducement Effect of Interferon Alpha on Apoptosis of Rat Vascular Smooth Muscle Cells    via P204 and RAS Signal Pathway
    LONG Xiangshu, WU Qiang, LIU Taihe, SONG Fang, HUANG Jing, TIAN Maobo, XIAO Yan
    2015, 34(12): 1555-1558. https://doi.org/10.3870/j.issn.1004-0781.2015.12.002
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    Objective To investigate the effect of interferon alpha (IFN-α) on apoptosis of vascular smooth muscle cells (VSMCs) in rats and the related mechanism. Methods The cells were divided into three group: group A, group B and group C.Group A was transfected with nonspecific siRNA, group B was intervened with IFN-α and transfected with nonspecific siRNA, and group C was intervened with IFN-α and transfected with IFI204 siRNA.All the cells were cultured for 48 h.The expression of P204 mRNA was determined by semiquantitative reverse transcription polymerase chain reaction (RT-PCR).P204, RAS protein levels, and phosphorylation levels of RAF and ERK were analyzed by Western blotting.The cell apoptosis was analyzed by flow cytometry with Annexin-V FITC/PI method. Results As compared with group A, the expression of P204 mRNA and protein in group B was up-regulated (P＜0.05), the cell apoptosis was increased (P＜0.05), in the process of the above, the expression of RAS protein was decreased (P＜0.05) and the phosphorylation levels of RAF and ERK were dropped (P＜0.05).In group C, the expression levels of P204 mRNA and protein were down-regulated (P＜0.05), and cell apoptosis was decreased (P＜0.05), the expression of RAS protein and the phosphorylation levels of RAF and ERK were increased (P＜0.05). Conclusion P204 and RAS signal pathway participates in IFN-α regulation of apoptosis of VSMCs in rats.
  • Determination of Components from Ampelopsis Grossedentata by HPLC and Inhibitory    Effect of Its on Angiotensin Converting Enzyme
    WU Yong, LUO Hong, WANG Rui, TAN Yongxia, HU Junjie, ZHANG Baohui, TIAN Xianxiang, ZHENG Guohua, ZHANG Xiaoyi
    2015, 34(12): 1559-1562. https://doi.org/10.3870/j.issn.1004-0781.2015.12.003
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    Objective To measure the inhibition of components from Ampelopsis grossedentata on angiotensin converting enzyme (ACE) by high performance liquid chromatograph. Methods By quantitative determination of hippuric acid, the ACE-catalyzed product from the substrate HIPPURYL-HIS-LEU (HHL), the inhibitory effects of the total flavonoids of Ampelopsis grossedentata, dihydromyricetin and myricetin were evaluated respectively.High performance liquid chromatograph analysis was conducted on a Welchrom C18 column (4.6 mm×250 mm, 5 μm), eluting with water : acetonitrile (each containing 0.1% formic acid and 0.1% triethylamine)＝78:22, at flow rate of 1.0 mL·min－1 under temperature of 30 ℃, the detection wavelength was set at 228 nm, and the injection volume was 10 μL. Results The injection amount of hippuric acid showed good linearity with peak area within the range of 0.05–5.00 μg (r ＝ 0.999 9), and the inhibitory rate of the total flavonoids of Ampelopsis grossedentata and dihydromyricetin reached more than 75% against ACE. Conclusion As the method exhibited, the total flavonoids of Ampelopsis grossedentata and dihydromyricetin have potent inhibitions against ACE.For being simple, sensitive and repeatable, this method can be used for the screening of ACE inhibitors in vitro.
  • Long-term Toxicity of Self-microemulsifying Granules of Brucea Javanica Oil on    Rat
    WANG Chunliu, LONG Kaihua, YANG Jie, TANG Tao, ZANG Qiaozhen, LI Tonghui, XIN Yongjie, LI Ye
    2015, 34(12): 1563-1568. https://doi.org/10.3870/j.issn.1004-0781.2015.12.004
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    Objective To test long-term toxicity of self-microemulsifying granules of Brucea javanica oil on SD rats . Methods The SD rats were divided into blank control group, high-, medium- and low-dose (144, 72 and 36 folds of dosage for human, respectively) of self-microemulsifying granules of Brucea javanica oil groups randomly (n＝30 each group).The weight, appetite, haematological indexes, blood biochemical indexes, visceral index and histopathologic changes of organs of rats were measured at time point after 3, 6 months treatment and 4 weeks after drug withdrawal, respectively. Results Compared with blank control group, the drug groups showed no significant differences in the appearance and weight of rats.With haematological indexes, the level of lymphocyte rate (LY) in high dose group was lower while the levels of monor (MO) in high dose group was higher, MO and basic cells rate(BA)in medium dose group were significantly higher than those in blank control group (P＜0.01) after 3-month long treatment.Hematocrit (HCT) in high dose group was lower, neut (NE) in medium dose group was higher than those in blank control group(P＜0.01) after 6-month long treatment.Those changed indexes recovered to be blank on the 4th week after withdrawal.No significant difference was obtained compared with blank control group(P＞0.05).In blood biochemical indexes, the levels of total protein (TP), albumin (G), glutamic-pyruvic transaminase (ALT) in high dose group and TP in medium dose group were higher than those in blank control group (P＜0.05).Glutamic-oxalacetic transaminase (AST) in high dose group was significantly higher than that in blank control group (P＜0.01) after 3 months.After 6 months treatment , compared with blank control group, the level of glutamic-oxalacetic transaminase (AST) in high dose group was higher while trilaurate glycerin (TG) was lower(P＜0.05).The level of creatine kinase (CK) was lower(P＜0.05) and the level of TG was significantly lower (P＜0.01).The level of glucose (Glu) was significantly lower (P＜0.01).Those changed indexes recovered to be blank on the 4th week after withdrawal.In haematological indexes,delayed enlargements of liver and testis occurred after withdrawal.No rat died in the process of experiment.No obvious histopathologic changes of organs of rats were observed . Conclusion Self-microemulsifying granules of Brucea javanica oil have no apparent toxicity.It is worthy of further research.
  • Antitussive and Expectorant Effects of Water Extract of Plumeria rubra in Mice
    QIN Xining, LI Yuanhui, HUANG Ying, GUO Shengqi, HUANG Tingzhang, LI Yunda, CHENG Shijia, HUANG Yanfeng, ZHAO Shanmin
    2015, 34(12): 1569-1571. https://doi.org/10.3870/j.issn.1004-0781.2015.12.005
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    Objective To explore the antitussive and expectorant effects of water extract of Plumeria rubra in mice. Methods The antitussive and expectorant effects in mice were observed by concentrated ammonia induced cough model and phenol red excretion method.The mice were divided into normal control group, Plumeria rubra water extract group (1.2, 2.1, 3.0 g·kg－1), and positive control group (carbetapentane citrate group and ammonium chloride group).The mice were intragastrically administrated according to per kg body weight dose.After corresponding drug administration for five days, latent phase of cough and cough frequency per 3 min were recorded in mice.The mice of phenol sulfonphthalein excretion test were administered for 5 days, and 1 h after final adminstration, 2.5% phenol sulfonphthalein solution was intraperitoneally injected to detect the concentration of tracheal phenol sulfonphthalein. Results Compared with normal control group, the latent phase was significantly longer in low, middle and high Plumeria rubra water extract group (F＝13.54, P＜0.05).The cough frequency per 3 min was significantly reduced in low Plumeria rubra water extract group and the carbetapentane citrate group (F＝4.98,P＜0.05); the concentration of phenol sulfonphthalein of mouse trachea in low and high Plumeria rubra water extract group was significantly increased (F＝6.98,P＜0.05).Latent phase of cough was longer in low and middle Plumeria rubra water extract group than in carbetapentane citrate group (F＝11.43,P＜0.05). Conclusion Plumeria rubra water extract has significant antititussive and expectorant efficacy.
  • Effect of Resveratrol on the Expression of Survivin and p27 Protein in Human Renal Cell    Carcinoma 786-0 Cells
    TU Rui , LIU Xiaoqiao
    2015, 34(12): 1572-1575. https://doi.org/10.3870/j.issn.1004-0781.2015.12.006
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    Objective To investigate the effect of resveratrol (Res) on the expression of survivin, p27 protein in human renal cell carcinoma 786-0 cells. Methods The cell cycle and apoptosis of 786-0 cells were detected by flow cytometry, and the expression of survivin and p27 protein of 786-0 cells were detected by Western blotting at time point of 12, 24 and 48 h after human renal cell carcinoma 786-0 cells were cultured with Res (25 μmol·L－1) and blank medium, respectively. Results The ratio in G1 phase [(64.87±3.76)%] and G2 phase [(9.91±0.82)%] in the experimental group decreased significantly (P＜0.05), and the ratio of S phase [(25.05±3.12)%] increased significantly (P＜0.05).The apoptosis rate of experimental group at 12, 24 and 48 h [(5.12±0.64)%, (5.71±0.49)%, (5.84±0.30)%] was significantly higher than that of the control group [(0.72±0.27)%, (0.93±0.25)%, (1.12±0.21)%, P＜0.05].The expression of survivin protein in experimental group was significantly higher than that of the control group (P＜0.05), and the expression was significantly increased with time prolonging (P＜0.05).The expression of p27 protein in experimental group was significantly lower than that of the control group (P＜0.05), and the expression level was significantly decreased in time-dependent manner (P＜0.05).There was a negative correlation between the expression of survivin and p27 protein in the experimental group (P＜0.05). Conclusion Res could inhibit the expression of p27 protein and up-regulate survivin expression level, and promote the apoptosis of renal cell carcinoma 786-0 cells.
  • Effect of Dibenzazepines Injection on Pulmonary Hypertension in Rats
    XIAO Qingping, LIU Jianwen, ZHANG Minhong, LI Fuhuan, GUO Dong
    2015, 34(12): 1576-1578. https://doi.org/10.3870/j.issn.1004-0781.2015.12.007
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    Objective To investigate the effect and mechanism of dibenzazepines (DBZ) injection on pulmonary hypertension in rat. Methods Rat models of pulmonary hypertension were established, and 30 male rats were randomly divided into 3 groups: normal control group with saline injection, pulmonary hypertension model control group with hypoxia treatment and saline injection, DBZ group with hypoxia treatment and DBZ injection.The right ventricular pressure was determined by ultrasound cardiogram.Pulmonary arterial remodeling was detected by HE staining.Proliferation cell nuclear antigen and CCK-8 in pulmonary arterial smooth muscle cells were detected by Western blotting. Results The right ventricular pressure of pulmonary hypertension group was significantly increased compared with normal control group [(4.60±0.16) kPa vs.(3.37±0.18) kPa(P＜0.01)].After hypoxia treatment, pulmonary arterial remodeling and proliferation of pulmonary arterial smooth muscle cells of the rats of pulmonary hypertension group were augmented remarkably.Rats from DBZ group showed reductions in right ventricular pressure, amelioration in pulmonary arterial remodeling and suppression in proliferation of pulmonary arterial smooth muscle cells.The proliferation of rat pulmonary artery smooth muscle cells decreased significantly in DBZ treated group [(2.073±0.064) vs.(4.392±0.013)] compared with model control group (P＜0.01). Conclusion Notch pathway takes part in the process of pulmonary hypertension, and DBZ injection can significantly suppress the proliferation of pulmonary artery smooth muscle cells with a protective effect on pulmonary hypertension.
  • Comparison of Antioxidant Activity Between Phenolic and Nonphenolic Alkaloids in    Nelumbo Nucifera Gaertn in vitro
    YANG Xiaoqing, SONG Jinchun, XIE Shunlan, HAO Haohua
    2015, 34(12): 1579-1584. https://doi.org/10.3870/j.issn.1004-0781.2015.12.008
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    Objective To compare the antioxidant activity between phenolic and nonphenolic alkaloids in Nelumbo Nucifera Gaertn.in vitro. Methods DPPH, ABTS, hydroxyl radical scavenging, super oxygen anion from oxidation, reducing power and beta carotene bleaching test methods were used to evaluate the antioxidant activity of the alkaloids. Results Half maximalinhibitory concentration (IC50) of DPPH among total alkaloid, phenolic alkaloids and nonphenolic alkaloids was 21.89, 27.10 and 32.87 μg·mL－1, respectively; IC50 of ABTS free radicals was 14.25, 20.55, 25.94 μg·mL－1; The hydroxyl radical scavenging IC50 was 0.03, 0.03, 0.08 μg·mL－1; The auto-oxidation rate of super oxygen was 8.72×10－4, 5.87×10－4, 6.68×10－4; The total alkaloid had the best reducing power, while the non-phennolic alakloids had the worst; Lipid inhibition rate of three alkaloids were 89.63%, 85.85% and 83.78% respectively. Conclusion Phenolic alkaloids are better than non-phenolic alkaloids in hydroxyl radical scavenging, reducing power and anti-lipid peroxidation, resulting in a promising prospect.
  • Effect of Osthole on Mast Cells and Expression of STAT5 Gene and Protein in Mice with    Eczema
    XIONG Jian,ZHONG Zhendong,FU Rong,XIONG Wei
    2015, 34(12): 1584-1587. https://doi.org/10.3870/j.issn.1004-0781.2015.12.009
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    Objective To explore the effect of osthole on mast cells and expression of STAT5 gene and protein in them. Methods Passive cutaneous anaphylaxis was made in mice to copy eczema model, then the allergic mast cells were separated, and the ovalbumin was used to induce allergy of mast cells.Different concentrations of osthole were used to intervene the sensitized mast cells.Then the sensitized mast cells were divided into blank control group, osthole high-dose group and low-dose group.At the end of the experiment, morphology of the mast cells was detected by immunofluorescence technology.MTT assay was used to detect the influence of drugs on mast cells proliferation.RT-PCR and Western blotting were used to detect the expression of STAT5 gene and protein. Results As compared with blank control group, the number of mast cells in the osthole groups was significantly reduced, cells and nuclei obviously shrank, even apoptosis of some cells could be observed; the inhibition rate of mast cells in osthole groups was significantly increased in concentration-dependent manner (P＜0.01).As compared with blank control group (2.16±0.57), gene expression of STAT5 was significantly decreased in osthole high-dose group (0.59±0.12) and low-dose group (0.82±0.13) (P＜0.01).The protein expression of STAT5 was also significantly decreased in osthole high-dose group and low-dose group as compared with blank control group (P＜0.01). Conclusion Osthole can inhibit the proliferation of sensitized mast cells, and reduce the expression of STAT5 gene and protein.
  • Evaluation of Therapeutic Effect of Recombinant Human Granulocyte-macrophage    Colony-stimulating Factor Combined with SD-Ag Unguent on Deep Second Degree    Burn
    WANG Bin, LUO Xu, TANG Congrong, ZHANG Xiuhua
    2015, 34(12): 1588-1590. https://doi.org/10.3870/j.issn.1004-0781.2015.12.010
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    Objective To evaluate the therapeutic effect of recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) combined with sulfadiazine silver (SD-Ag) unguent on deep second degree burn. Methods Eighty-nine patients with deep second-degree burn (burns areas＜10%) were enrolled.These patients were divided into two groups at random (Group A and Group B).The patients in Group A were treated with SD-Ag unguent only, and those in Group B were treated with rhGM-CSF and SD-Ag unguent.The therapeutic effects and the adverse drug reaction were recorded. Results The healing time in Group A [(19.79±1.47) days] was obviously shorter than that in Group B [(15.76±1.63) days].The total wound healing rates in Group B [on day 9: (76.41±3.24)%, day 13: (95.01±1.43)%, day 16: (99.54±0.88)%, and day 21 (100.00±0.00)%] were higher than those of Group A [on day 9: (67.24±2.33)%, day 13: (75.54±1.11)%, day 16:(88.33±1.32)%, day 21: (99.14±1.95)%].During the treatment, there was no obvious adverse reaction was observed in the two groups. Conclusion The application of rhGM-CSF combined with SD-Ag unguent can not only accelerate the healing rates of deep second-degree burn, but also has curative effect with safety.
  • Effect of Compound Nitroglycerin Gel on Skin Ulcer Wound in Rats
    CHENG Jing, MEI Rui, LIU Zhong, LIU Ping,MEI Liping, SONG Hongping
    2015, 34(12): 1591-1594. https://doi.org/10.3870/j.issn.1004-0781.2015.12.011
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    Objective To study the protective effect of compound nitroglycerin gel on rats with skin ulcer wound and its action mechanism. Methods Skin ulcer model of 54 rats was established.Then the rats were randomly divided into three groups (n＝18 each), including model control group, Jin wan hong group, and compound nitroglycerin gel group.Wound healing process and healing time were recorded.At the day 7 and 14 after the model was established, the number of fibroblasts and new blood capillaries of granulation tissue from center of the wound were measured,and RT-PCR was used to detect mRNA expression levels of VEGF, Ang1 andHIF-1α. Results As compared with model control group [(24.17±5.91) days], healing time of skin in the compound nitroglycerin gel group was significantly shorter [(14.67±3.76) days, P＜0.01].The numbers of fibroblasts (61.20±7.56) and new blood capillaries (9.35±1.43) were increased, and mRNA expression levels of VEGF (1.692±0.196), HIF-1α (1.527±0.174) and Ang1 (1.548±0.203) were remarkably up-regulated on 7th day (P＜0.05 or P＜0.01).While on 14th day, the numbers of fibroblasts (28.00±5.96) and new blood capillaries (4.20±1.30) were decreased and the mRNA expression levels of VEGF (1.156±0.123), HIF-1α (1.021±0.105) and Ang1 (1.034±0.134) were significantly down-regulated (P＜0.05 or P＜0.01). Conclusion Compound nitroglycerin gel can treat skin ulcer wound via regulating the numbers of fibroblasts, new blood capillaries and VEGF/Ang1/HIF-1α signal transduction pathway.
  • Investigation of Feedback Regulation of Close-loop Muscle Relaxant Injection System on    Accuracy of Cisatracurium Besilate Usage
    GUO Rui, LI Jianbin, WANG Lixun, HE Wanwen, LI Hui, CHEN Youli
    2015, 34(12): 1599-1602. https://doi.org/10.3870/j.issn.1004-0781.2015.12.013
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    Objective To investigate feedback regulation of close-loop muscle relaxant injection system on accuracy of cisatracurium besilate usage. Methods Two hundred patients undergoing laparoscopic cholecystectomy surgery, aged 20 to 40 years old, at ASA Ⅰ or Ⅱ, were randomly divided into two groups: control group and treatment group (n＝100 each group).In the control group, the patients received injection of cisatracurium besilate with closed-loop muscle relaxant injection system at 1.5–2.0 μg·kg－1·min－1, until 30 min before the end of surgery; if the muscle relaxant level could not meet the requirement of the operation, extra 0.05 mg·kg－1 was added.The treatment group was adopted closed-loop muscle relaxant monitoring under negative feedback regulation of infusion cisatracurium, and the close-loop control parameters were set to: drug was added when TOF was 8%, and injection speed was 2.5 μg·kg－1·min－1, maintaining speed was 0.33 μg·kg－1·min－1, the stimulus current for monitoring muscle relaxant was 60 mA , and the pulse width was 200 μs.The Cooper score, cisatracurium dosage, and muscle recovery index, TOFr75 and TOFr90 of the two groups were compared.Prediction probability (Pk) of NI on awakening period of eye opening and directional force recovery of the two groups were detected, and regression equation was established to predict ED50 and ED95 related NI . Results Cooper score was significantly higher in the treatment group than in the control group (P＜0.01).Muscle recovery index, TOFr75, TOFr90, and cisatracurium dosage per unit time and body mass were significantly lower in the treatment group than in the control group(P＜0.01). Pk of NI on awakening period of eye opening and directional force recovery of the two groups were higher than 0.5; and Pk of the treatment group were significantly higher than those of the control group (P＜0.01). Regression equation predicted that ED95 was significantly lower in the treatment group than in the control group (P＜0.01), while the ED50 between the two groups has no significant difference (P＞0.05). Conclusion The accuracy of closed loop muscle relaxant injection system is higher than that of the traditional method, it provides better muscle relaxation effect for tracheal intubation, reduces recovery time, increases the Pk of NI on patient awakening.
  • HPLC Fingerprint Analysis of Disporum Cantoniense Rhizoma
    GAN Xiuhai, LIANG Zhiyuan, ZHOU Xin, ZHAO Chao, WEI Gang
    2015, 34(12): 1623-1626. https://doi.org/10.3870/j.issn.1004-0781.2015.12.019
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    Objective To establish the HPLC fingerprint of Disporum cantoniense Rhizoma. Methods HPLC analysis was performed on Agilent Zorbax SB-C18 chromatographic column (4.6 mm×250 mm, 5 μm) with the mobile phase consisting of methanol-0.05% phosphoric acid in gradient mode.The flow rate was 1.0 mL·min-1, the detection wavelength was 256 nm and the column temperature was 30 ℃. Results The HPLC fingerprint of 15 batches of Disporum cantoniense Rhizoma was established.Thirteen common peaks in the fingerprint were demarcated, four of which were identified by reference substances.Chemical pattern recognition of fingerprint was performed by hierarchical cluster analysis and principal component analysis. Conclusion The method is simple, accurate and has a good repeatability, and can be used for quality control of Disporum cantoniense Rhizoma.
  • Formulation Screening and Determination of Troxerutin Microemulsion
    XU Man, YU Qing, ZHAO Qianru, CHEN Wei, LIN Yuanjie, JIN Yong
    2015, 34(12): 1627-1632. https://doi.org/10.3870/j.issn.1004-0781.2015.12.020
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    Objective To optimize a W/O microemulsion formulation of troxerutin and evaluate its physical properties such as morphology, droplet size, stability and content of troxerutin. Methods The W/O microemulsion was optimized using a pseudoternary phase diagram and the area of the microemulsion region was used to screen and determine microemulsion components.HPLC assay was used for determination of the loading content. Results The optimal formulation contained lecithin, ethanol, isopropyl myristate and water (23.30:11.67:52.45:12.59).The microemulsion was physicochemically stable with round shape and uniform size, and the mean droplet size was about 50.20 nm. Conclusion Microemulsion was developed successfully.It will expect to be the new preparation for troxerutin.
  • Rapid Determination of Moisture Content in Polygoni multiflori Radix by Near-    Infrared Spectroscopy
    JIA Canchao, LU Huijuan, LIN Dan, JI Shengguo
    2015, 34(12): 1633-1636. https://doi.org/10.3870/j.issn.1004-0781.2015.12.021
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    Objective To determine the moisture content in Polygoni multiflori Radix rapidly by near-infrared spectroscopy. Methods The moisture content of the samples were determined by oven drying method and the near-infrared spectrum data were collected by near-infrared spectroscopy.The quantitative test model of moisture content in Polygoni multiflori Radix was established by chemometrics methods and was validated with validation samples. Results The correlation coefficients, root-mean-square error of calibration (RMSEC), root-mean-square error of predication (RMSCP) and the root-mean-square error of cross-validation (RMSECV) of the calibration model was 0.984 75, 0.161, 0.181 and 0.471 64, respectively.The absolute deviation of the analytical and predictive values of 21 validation samples was －0.35%－0.28%, and the average recovery was 99.87%. Conclusion Near-infrared spectroscopy can be used to determine the moisture content of Polygoni multiflori Radix rapidly and accurately.
  • Transforming Process of Shikonin
    ZHOU Jian, GUO Ruixia, WANG Ruxing, ZHAO Guiqin
    2015, 34(12): 1637-1639. https://doi.org/10.3870/j.issn.1004-0781.2015.12.022
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    Objective To investigate the optimal condition for transforming alkanna tinctoria pigment into shikonin. Methods Transformation rate of shikonin served as index.Transformation temperature, time, ratio of 2% NaOH to alkanna tinctoria pigment (v/w) was optimized. Results With ratio of 2% NaOH to alkanna tinctoria pigment being 4.5 mL·mg－1, temperature 35 ℃ and the reaction time 4 h, the transformation rate reached the highest, and the average transformation rate was 64.86%. Conclusion This method is easy and simple, and suitable for industrialized production.
  • Quality Standard for Compound Chaihu Tea
    XU Benshan, LEI Ning, CHEN Jingjie, CHANG Ziqian, MA Ping, TONG Weihang
    2015, 34(12): 1640-1643. https://doi.org/10.3870/j.issn.1004-0781.2015.12.023
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    Objective To improve the quality standard of compound Chaihu tea. Methods Pupleuri Radix, Puerariae Lobatae Radix and Forsythiae Fructus were identified by thin layer chromatography (TLC).HPLC method was used to determine the content of puerarin.The determination was performed on a Shimadzu VP-ODS-C18 column (250 mm×4.6 mm,5 μm) with the mobile phase of methanol-0.2% H3PO4 (23:77).The flow rate was 1.0 mL·min－1, the column temperature was set at 30 ℃ and the detection wavelength was 250 nm. Results The TLC spots were clear and well-separated without negative interference.The good linear correlation existed within the range of 1.340 8–56.632 8 μg· mL－1 for puerarin (r＝0.999 9, n＝6) and the average recovery was 102.98% with RSD being 1.48% (n＝6). Conclusion The method is simple and accurate.It can be used for quality control of compound Chaihu tea.
  • Quality Control of Compound Cornu Cervi Degelatinatum Tablets
    ZHANG Yuwei, WANG Yanyan, WANG Yafen, ZHANG Kailin
    2015, 34(12): 1644-1648. https://doi.org/10.3870/j.issn.1004-0781.2015.12.024
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    Objective To establish a method of quality control for compound Cornu cervi degelatinatum tablets. Methods Thin layer chromatography (TLC) was used for qualitative identification of Lonicerae Japonicae Caulis and Pyrola Herba in the preparations.High performance liquid chromatography was used to determine the content of loganin and chiratin in Lonicerae Japonicae Caulis and monotropein in Pyrola Herba. Results The TLC spots were clear and specific in the qualitative identification.HPLC determined the content.In the concentration range, monotropein, loganin and chiratin had a good linear relationship with peak area (r＞0.999).The mean recovery was 97.2%, 98.4% and 96.0%; RSD was 1.4%, 0.8% and 0.8%. Conclusion The present study employs TLC for quality control and HPLC for quantity control.The methods are simple and accurate, have good reproducibility, and can effectively control the quality of compound Cornu cervi degelatinatum tablets.
  • Quality Standard of Shenbo Fuyanning Suppository
    FENG Songqiao, FEI Yiqin
    2015, 34(12): 1649-1651. https://doi.org/10.3870/j.issn.1004-0781.2015.12.025
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    Objective To establish a method for quality control of Shenbo Fuyanning suppositories. Methods Sophorae flavescentis Radix, Dioscoreae hypoglaucae Rhizoma, and Common cnidium Fruit were identified by thin layer chromatography (TLC), and the content of berberine hydrochloride was determined by HPLC. Results The TLC spots were clear and the negative control had no interference.Berberine hydrochloride had a linear relationship in the range of 0.079－0.790 μg, and the average recovery was 99.3%(RSD＝0.70%,n＝6). Conclusion The method is accurate, reliable and feasible, and can be used in the quality control of the Shenbo Fuyanning suppository effectively.
  • Study on Compatibility Stability of Alanylglutamine by Using Continuous Series Infusion    Device
    ZHANG Chengliang,FENG Chengyang,XU Yanjiao,YU Zaoqin,LIU Dong
    2015, 34(12): 1657-1659. https://doi.org/10.3870/j.issn.1004-0781.2015.12.028
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    Objective To investigate the compatible stability of alanylglutamine by using continuous series infusion device,and provide experimental evidence for reasonable clinical use of alanylglutamine. Methods pH,osmolality and quantity of insoluble particles were measured by using pH meter, automatic freezing osmometer and intelligent particle detector respectively.A HPLC method was built for the determination of the content of alanylglutamine. Results pH and quantity of insoluble particles of the two tested groups did not change significantly over time.Osmolality and the content of alanylglutamine fluctuated greatly in the first half an hour. Conclusion Continuous series infusion device may not mix each bottle of solution very well.It is suggested to premix these solutions to ensure the stability of the dilution ratio and the osmotic pressure of the mixture in the process of the infusion.
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